FastQCFastQC Report
Fri 10 Feb 2017
ERR1631399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750191
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31210.4160273850259467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24110.3213848206656705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22380.29832402681450454No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11250.14996180972578982No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9280.12370183060047375No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC9270.12356853121405081No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9270.12356853121405081No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT9160.12210223796339865No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.11703686127932753No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8420.11223808336810225No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC8060.10743930545687698No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT7890.10517321588768727No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG7730.10304042570492047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT400.001930565123.12512
GTAAGGT400.001930565123.12519
TAGACTA450.00382458920.5555535
GTATACT450.00382458920.5555534
GGTATCA13550.019.3874551
GCAGTCG2200.019.3409089
TGTCCGA1551.8189894E-1219.09677312
CAGTCGG2150.018.93023310
TTTAGAC701.2184808E-418.53
TCGGGTA500.00703265818.525
CTAACAC500.00703265818.53
TAAGGTT609.232737E-418.54
GGTAGTA609.232737E-418.515
ATACACA2200.018.537
CCGCTTA902.1501892E-618.49999825
CTCTATG3500.017.971431
AGTCGGT2300.017.69565211
GGAACGT1054.791764E-717.61904710
GTTCAAA3050.017.5901641
TCGGTGA2250.017.26666613
Produced by FastQC (version 0.11.5)