##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631399.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	750191
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.051350922631705	34.0	33.0	34.0	31.0	34.0
2	33.183221339632176	34.0	33.0	34.0	31.0	34.0
3	33.26691869137326	34.0	34.0	34.0	31.0	34.0
4	36.48376080224903	37.0	37.0	37.0	35.0	37.0
5	36.477381093614824	37.0	37.0	37.0	35.0	37.0
6	36.53516904361689	37.0	37.0	37.0	35.0	37.0
7	36.544993208396264	37.0	37.0	37.0	35.0	37.0
8	36.544622636102005	37.0	37.0	37.0	35.0	37.0
9	38.39745344852177	39.0	39.0	39.0	37.0	39.0
10	38.35660918352793	39.0	39.0	39.0	37.0	39.0
11	38.409679668244486	39.0	39.0	39.0	37.0	39.0
12	38.37399942148066	39.0	39.0	39.0	37.0	39.0
13	38.39797464912269	39.0	39.0	39.0	37.0	39.0
14	39.93382085362261	41.0	40.0	41.0	38.0	41.0
15	39.92992584555133	41.0	40.0	41.0	38.0	41.0
16	39.8838789055054	41.0	40.0	41.0	38.0	41.0
17	39.88498129143112	41.0	40.0	41.0	38.0	41.0
18	39.853815894885436	41.0	40.0	41.0	38.0	41.0
19	39.868456166496266	41.0	40.0	41.0	38.0	41.0
20	39.8293008047284	41.0	40.0	41.0	38.0	41.0
21	39.8034273938237	41.0	40.0	41.0	38.0	41.0
22	39.73759749183875	41.0	40.0	41.0	38.0	41.0
23	39.66641695248277	41.0	40.0	41.0	37.0	41.0
24	39.6229946773555	41.0	40.0	41.0	37.0	41.0
25	39.61528863982639	41.0	40.0	41.0	37.0	41.0
26	39.511247135729434	41.0	40.0	41.0	37.0	41.0
27	39.44865640883455	41.0	39.0	41.0	36.0	41.0
28	39.363258156922704	41.0	39.0	41.0	36.0	41.0
29	39.30247630270158	41.0	39.0	41.0	35.0	41.0
30	39.220851756419364	41.0	39.0	41.0	35.0	41.0
31	39.157146646653985	41.0	39.0	41.0	35.0	41.0
32	39.07043006381041	41.0	39.0	41.0	35.0	41.0
33	38.967938831577555	41.0	39.0	41.0	35.0	41.0
34	38.91013755163685	41.0	39.0	41.0	35.0	41.0
35	38.78508006627646	40.0	38.0	41.0	35.0	41.0
36	38.71402749433145	40.0	38.0	41.0	35.0	41.0
37	38.63501295003539	40.0	38.0	41.0	35.0	41.0
38	38.53655535723569	40.0	38.0	41.0	35.0	41.0
39	38.43543310970139	40.0	38.0	41.0	35.0	41.0
40	38.311688623297265	40.0	37.0	41.0	35.0	41.0
41	38.204915814772505	40.0	37.0	41.0	35.0	41.0
42	38.04442068753158	40.0	37.0	41.0	34.0	41.0
43	37.21890958435918	40.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	2.0
13	3.0
14	2.0
15	5.0
16	3.0
17	15.0
18	17.0
19	38.0
20	67.0
21	120.0
22	179.0
23	356.0
24	531.0
25	800.0
26	1194.0
27	1630.0
28	2369.0
29	3323.0
30	4302.0
31	5470.0
32	7057.0
33	9581.0
34	13834.0
35	20856.0
36	34083.0
37	67401.0
38	142826.0
39	434122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.651816670687865	17.546464833622373	12.291936320217118	26.509782175472647
2	19.753369475240305	20.248843294574314	32.59130008224572	27.406487147939657
3	20.487582495657772	21.560775855748737	28.76894017656837	29.182701472025123
4	15.008577815516317	15.394346239824259	32.540513016018586	37.056562928640844
5	16.540187765515714	34.5094782528716	31.788971075366142	17.161362906246545
6	34.89777936552158	33.11356707825074	15.92181191189977	16.066841644327912
7	30.55501865524913	29.18936644134627	20.11381101612789	20.14180388727671
8	28.998748318761493	30.42745114244239	19.56808332811244	21.00571721068368
9	27.783724411516538	13.869667857918849	18.99236327815183	39.354244452412786
10	19.32747793561906	24.701842597418526	29.728029261881307	26.24265020508111
11	37.78384438096432	20.934535338333838	19.828816927955682	21.452803352746166
12	23.680902596805346	24.753296160577772	26.341958247966186	25.223842994650695
13	31.005570581358615	17.77200739544996	24.563344534925104	26.65907748826632
14	24.306743216060976	20.157666514261035	22.951888252458374	32.583702017219615
15	27.400488675550626	25.827289316987272	20.711925363007555	26.060296644454546
16	26.932741128592586	24.475233640499553	22.726878888176476	25.865146342731382
17	25.84168565072095	24.92458587213123	23.381645474285882	25.852083002861935
18	25.66399756861919	22.696220029299205	25.4128615245984	26.226920877483202
19	26.016307846934982	24.12825533764068	24.720771110290578	25.134665705133756
20	26.51564734847526	22.61424090664911	24.60360094962483	26.26651079525081
21	27.88929752556349	23.5747962852127	23.739954224990704	24.795951964233108
22	27.53552095399705	22.908565951870923	23.432432540513016	26.12348055361901
23	25.783833717013398	23.077722873241616	24.519489036791963	26.61895437295302
24	25.433522929493957	24.203702790356054	24.3935211166223	25.96925316352769
25	25.894872105903698	23.029201896583672	24.22303120138738	26.852894796125256
26	25.86407994764	24.53255237666141	24.33286989579987	25.270497779898722
27	26.951269743305375	22.825520434129444	23.84099515989928	26.38221466266591
28	25.03522436286226	23.852858805290918	25.04455531991186	26.067361511934962
29	24.766492799833642	23.906445158632934	25.347544825251166	25.979517216282254
30	24.201436700786864	24.992168661047653	25.51017007668714	25.296224561478343
31	25.804228523136107	24.32380553752311	23.84912642247108	26.022839516869706
32	24.71677212869789	23.703030294951553	24.47470044295386	27.105497133396696
33	24.264354011178487	23.21648753450788	25.42832425342346	27.090834200890175
34	25.751308666726207	23.19316014188387	25.018428640172967	26.037102551216957
35	25.643202864337216	23.11398030634865	25.882208664193517	25.360608165120617
36	23.82926481389406	24.118924380591075	25.69665591829281	26.355154887222053
37	25.031225381269568	22.47720913740634	26.152673119245634	26.338892362078457
38	24.00362041133525	21.894824118124586	27.3299733001329	26.77158217040727
39	24.412316330107934	21.565308034887114	27.385825743044105	26.636549891960847
40	23.520943333097836	22.062514746244624	28.211348843161275	26.205193077496265
41	22.358039485944246	22.147026557236757	29.471694541790022	26.02323941502897
42	23.429233355238864	21.996531549965276	29.105787726059095	25.46844736873676
43	21.755392959926205	21.313772092707055	29.460364093944076	27.47047085342266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	116.0
1	113.0
2	110.0
3	228.5
4	347.0
5	347.0
6	489.5
7	632.0
8	605.0
9	578.0
10	958.0
11	1338.0
12	1338.0
13	2226.0
14	3114.0
15	4871.5
16	6629.0
17	5963.0
18	5297.0
19	5297.0
20	5803.0
21	6309.0
22	5390.5
23	4472.0
24	4717.5
25	4963.0
26	4963.0
27	5507.5
28	6052.0
29	7553.0
30	9054.0
31	10601.0
32	12148.0
33	12148.0
34	14661.5
35	17175.0
36	20503.0
37	23831.0
38	27689.0
39	31547.0
40	31547.0
41	34192.0
42	36837.0
43	37873.0
44	38909.0
45	42483.5
46	46058.0
47	46058.0
48	49352.5
49	52647.0
50	55221.0
51	57795.0
52	60015.0
53	62235.0
54	62235.0
55	58857.5
56	55480.0
57	53475.0
58	51470.0
59	49052.5
60	46635.0
61	46635.0
62	43979.0
63	41323.0
64	38412.5
65	35502.0
66	31701.0
67	27900.0
68	27900.0
69	24176.5
70	20453.0
71	18085.5
72	15718.0
73	12257.5
74	8797.0
75	8797.0
76	7210.5
77	5624.0
78	4841.0
79	4058.0
80	3648.5
81	3239.0
82	3239.0
83	2621.5
84	2004.0
85	1893.0
86	1782.0
87	1556.5
88	1331.0
89	1331.0
90	962.0
91	593.0
92	326.0
93	59.0
94	37.5
95	16.0
96	16.0
97	12.5
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	750191.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.18559961283571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.39507981630669	45.24555163222129
2	5.9413506483808085	6.082231908949638
3	1.700529127344791	2.611278091267061
4	0.8424635209579411	1.72488001888692
5	0.5288059845536002	1.3533625699115979
6	0.3449104819743523	1.0592669899565632
7	0.2560771631608473	0.917522420047935
8	0.2054536135048209	0.8413013119894446
9	0.16384958875875996	0.7548065502240306
>10	1.2658669279806025	13.493917085199088
>50	0.19297245437412291	6.869350437155459
>100	0.15401182672323493	15.270765141126127
>500	0.007582925254619784	2.5852572764042354
>1k	0.0010459207247751428	1.1905085666605755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3121	0.4160273850259467	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2411	0.3213848206656705	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2238	0.29832402681450454	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1125	0.14996180972578982	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	928	0.12370183060047375	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	927	0.12356853121405081	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	927	0.12356853121405081	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	916	0.12210223796339865	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	878	0.11703686127932753	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	842	0.11223808336810225	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	806	0.10743930545687698	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	789	0.10517321588768727	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	773	0.10304042570492047	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.3329938642292428E-4
6	0.0	0.0	0.0	0.0	1.3329938642292428E-4
7	0.0	0.0	0.0	0.0	1.3329938642292428E-4
8	0.0	0.0	0.0	0.0	1.3329938642292428E-4
9	0.0	0.0	0.0	0.0	1.3329938642292428E-4
10	0.0	0.0	0.0	0.0	1.3329938642292428E-4
11	0.0	0.0	0.0	1.3329938642292428E-4	1.3329938642292428E-4
12	0.0	0.0	0.0	3.998981592687729E-4	1.3329938642292428E-4
13	0.0	0.0	0.0	6.664969321146215E-4	1.3329938642292428E-4
14	0.0	0.0	0.0	0.001332993864229243	1.3329938642292428E-4
15	0.0	0.0	0.0	0.0015995926370750916	1.3329938642292428E-4
16	0.0	0.0	0.0	0.0025326883420355617	1.3329938642292428E-4
17	0.0	0.0	0.0	0.004265580365533577	1.3329938642292428E-4
18	0.0	0.0	0.0	0.004798777911225275	1.3329938642292428E-4
19	0.0	0.0	0.0	0.005331975456916972	1.3329938642292428E-4
20	0.0	0.0	0.0	0.0063983705483003665	1.3329938642292428E-4
21	0.0	0.0	0.0	0.007198166866837912	1.3329938642292428E-4
22	0.0	0.0	0.0	0.009330957049604701	1.3329938642292428E-4
23	0.0	0.0	0.0	0.011730346005217338	1.3329938642292428E-4
24	0.0	1.3329938642292428E-4	0.0	0.017328920234980158	1.3329938642292428E-4
25	0.0	1.3329938642292428E-4	0.0	0.02052810550913034	1.3329938642292428E-4
26	0.0	1.3329938642292428E-4	0.0	0.028126170535237027	1.3329938642292428E-4
27	0.0	1.3329938642292428E-4	0.0	0.06025132266316178	1.3329938642292428E-4
28	0.0	1.3329938642292428E-4	0.0	0.18035406983021657	1.3329938642292428E-4
29	0.0	1.3329938642292428E-4	0.0	0.33764734580926725	1.3329938642292428E-4
30	0.0	1.3329938642292428E-4	0.0	0.5530591542687129	1.3329938642292428E-4
31	0.0	1.3329938642292428E-4	0.0	1.1134497747906866	1.3329938642292428E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAT	40	0.0019305651	23.125	12
GTAAGGT	40	0.0019305651	23.125	19
TAGACTA	45	0.003824589	20.555553	5
GTATACT	45	0.003824589	20.555553	4
GGTATCA	1355	0.0	19.387455	1
GCAGTCG	220	0.0	19.340908	9
TGTCCGA	155	1.8189894E-12	19.096773	12
CAGTCGG	215	0.0	18.930233	10
TTTAGAC	70	1.2184808E-4	18.5	3
TCGGGTA	50	0.007032658	18.5	25
CTAACAC	50	0.007032658	18.5	3
TAAGGTT	60	9.232737E-4	18.5	4
GGTAGTA	60	9.232737E-4	18.5	15
ATACACA	220	0.0	18.5	37
CCGCTTA	90	2.1501892E-6	18.499998	25
CTCTATG	350	0.0	17.97143	1
AGTCGGT	230	0.0	17.695652	11
GGAACGT	105	4.791764E-7	17.619047	10
GTTCAAA	305	0.0	17.590164	1
TCGGTGA	225	0.0	17.266666	13
>>END_MODULE