FastQCFastQC Report
Fri 10 Feb 2017
ERR1631398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941972
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81240.862446017503705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58710.623266933624354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57570.6111646630685413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.20818028561358512No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA17180.18238334048145805No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA16780.17813692976012027No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC15490.1644422551838059No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11640.12357055199092967No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG11490.12197814797042801No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11260.11953646180565877No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT10870.11539621135235442No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10720.11380380733185275No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC10300.10934507607444809No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC9490.10074609436373905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT10350.022.5217411
GCAGTCG10750.022.1999999
CAGTCGG10700.021.95794310
GGCAGTC11050.021.5972868
TCTATCG603.725369E-521.58333231
TCGGTGA10850.021.31336413
GGGCAGT11550.020.9826837
GTCGGTG11150.020.73991212
TATTCGT450.003825302520.5555576
CGGTGAT11250.020.55555514
TTCGTAC555.142145E-420.181828
AGTCTGC555.142145E-420.181828
GGTGATT11800.019.75423815
GATTCCT11450.019.55021718
CGCCTTA8050.019.53416325
ATTCCTC11700.019.29059819
GGTATCA31850.019.2841451
GCCTTAT8250.019.06060626
TTCCTCG8350.019.05389220
GTGATTC11950.019.04184216
Produced by FastQC (version 0.11.5)