##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631398.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	941972
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02472791123303	34.0	33.0	34.0	31.0	34.0
2	33.1472219981061	34.0	33.0	34.0	31.0	34.0
3	33.21988127035623	34.0	33.0	34.0	31.0	34.0
4	36.47190256185959	37.0	37.0	37.0	35.0	37.0
5	36.47572751631683	37.0	37.0	37.0	35.0	37.0
6	36.54583788053148	37.0	37.0	37.0	35.0	37.0
7	36.54951102580544	37.0	37.0	37.0	35.0	37.0
8	36.55268627942232	37.0	37.0	37.0	35.0	37.0
9	38.37821931012812	39.0	39.0	39.0	37.0	39.0
10	38.336566267362514	39.0	39.0	39.0	37.0	39.0
11	38.394823837651224	39.0	39.0	39.0	37.0	39.0
12	38.357053075887606	39.0	39.0	39.0	37.0	39.0
13	38.387488163130115	39.0	39.0	39.0	37.0	39.0
14	39.88703167397757	41.0	40.0	41.0	38.0	41.0
15	39.873053551485604	41.0	40.0	41.0	38.0	41.0
16	39.846732174629395	41.0	40.0	41.0	38.0	41.0
17	39.85385022060104	41.0	40.0	41.0	38.0	41.0
18	39.87608442713796	41.0	40.0	41.0	38.0	41.0
19	39.9072414042031	41.0	40.0	41.0	38.0	41.0
20	39.903521548411206	41.0	40.0	41.0	38.0	41.0
21	39.87602710059323	41.0	40.0	41.0	38.0	41.0
22	39.82105412899747	41.0	40.0	41.0	38.0	41.0
23	39.75857138004102	41.0	40.0	41.0	38.0	41.0
24	39.73219267664007	41.0	40.0	41.0	37.0	41.0
25	39.71302968665735	41.0	40.0	41.0	37.0	41.0
26	39.62432959790737	41.0	40.0	41.0	37.0	41.0
27	39.56293180689023	41.0	40.0	41.0	37.0	41.0
28	39.47154692496168	41.0	39.0	41.0	36.0	41.0
29	39.40036965005329	41.0	39.0	41.0	36.0	41.0
30	39.29884009291147	41.0	39.0	41.0	35.0	41.0
31	39.20090830725329	41.0	39.0	41.0	35.0	41.0
32	39.0995878858395	41.0	39.0	41.0	35.0	41.0
33	38.99484804219234	41.0	39.0	41.0	35.0	41.0
34	38.917169512469584	41.0	39.0	41.0	35.0	41.0
35	38.80368418594183	40.0	38.0	41.0	35.0	41.0
36	38.72175818389506	40.0	38.0	41.0	35.0	41.0
37	38.63311966810054	40.0	38.0	41.0	35.0	41.0
38	38.52802524915815	40.0	38.0	41.0	35.0	41.0
39	38.39848849010374	40.0	38.0	41.0	35.0	41.0
40	38.253436407876244	40.0	38.0	41.0	35.0	41.0
41	38.1224707316141	40.0	37.0	41.0	35.0	41.0
42	37.940343237378606	40.0	37.0	41.0	34.0	41.0
43	37.134925454259786	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	3.0
13	6.0
14	8.0
15	9.0
16	13.0
17	14.0
18	20.0
19	26.0
20	61.0
21	83.0
22	168.0
23	291.0
24	501.0
25	868.0
26	1277.0
27	1936.0
28	2977.0
29	4372.0
30	5634.0
31	6965.0
32	8750.0
33	11238.0
34	16925.0
35	24450.0
36	40269.0
37	90196.0
38	186300.0
39	538606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.13326510766775	17.66995197309474	13.614417413681085	24.582365505556428
2	18.491101646333437	20.68660214953311	34.836067314102756	25.9862288900307
3	19.297176561511385	23.2195861448111	29.672219556419936	27.811017737257583
4	13.059623852938303	16.059500707027386	34.91218422628273	35.968691213751576
5	14.531748289758081	37.2971808079221	32.54512872994102	15.625942172378796
6	32.22579864369642	35.438951476264684	17.14233544096852	15.192914439070377
7	29.641751559494338	31.066846997575297	20.470566004085047	18.820835438845315
8	30.142509543808092	31.058035695328524	19.323079666911543	19.476375093951837
9	28.17058256508686	14.079293227399543	19.401956746060392	38.34816746145321
10	18.834105472349496	26.200247990386128	29.581558687519376	25.384087849745
11	36.0268670406339	22.984441151117018	19.87861634953056	21.11007545871852
12	23.77193801938911	27.117472706195088	26.856318446832816	22.254270827582985
13	31.30061190778494	18.70915483687413	25.378567515807266	24.61166573953366
14	23.146760200940157	20.814631432781443	25.26964708080495	30.768961285473456
15	26.429872650142467	27.411324328111665	21.97464468158289	24.184158340162977
16	24.84925241939251	25.944932545765692	24.299660711783368	24.906154323058434
17	23.264704258725313	27.202825561693977	24.844793688135102	24.687676491445607
18	24.151142496804574	23.735100406381505	27.666108971391928	24.447648125421985
19	24.654129846747036	25.668385047538568	26.96279719567036	22.714687910044034
20	25.81148908884765	23.30430203870179	26.81948083382521	24.06472803862535
21	27.635534814198298	23.766205364915304	25.966695400712545	22.63156442017385
22	26.543570297206287	23.650809153562953	25.737495382028342	24.06812516720242
23	24.359959744026362	24.05485513369824	26.12636044383485	25.45882467844055
24	23.433286764362425	25.120810385022057	27.361429002135946	24.084473848479572
25	23.68340035584922	24.569520113124383	26.544738060154653	25.202341470871747
26	24.316221713596583	25.38610489483764	26.685612735835036	23.612060655730744
27	24.780460565706836	23.998908672444617	26.304072732522837	24.91655802932571
28	23.23784571091285	24.876535608277102	27.3539977833736	24.531620897436444
29	23.014802987774583	25.186948232006895	27.88023423201539	23.918014548203132
30	22.850360732590776	25.65957374529179	27.814733346638754	23.675332175478676
31	23.561316047610756	25.846097336226553	26.379765003630677	24.212821612532007
32	22.980831702003883	24.86071772834012	27.167580352706878	24.990870216949123
33	22.532092249026512	24.548712700589824	27.948813765164992	24.970381285218668
34	24.394886472209365	24.09052498375748	26.94719163626944	24.567396907763715
35	23.748901241225852	24.261655335827392	27.483937951446542	24.505505471500214
36	21.8864254988471	25.490141957510414	27.745410691612914	24.878021852029573
37	23.223938715800468	24.109527671735464	28.068456387238687	24.598077225225378
38	22.57211467007512	23.25536215513837	28.862535192128853	25.30998798265766
39	23.251328064953096	22.905033270628003	28.878034591261738	24.965604073157163
40	21.96201160968691	23.414814877724606	29.205008216804746	25.41816529578374
41	20.552946372079	24.04954712029657	30.441563018858307	24.95594348876612
42	21.787059487967795	23.75251069033899	29.96596501806848	24.494464803624734
43	20.383833065101722	23.675756816550813	30.06246470171088	25.87794541663659
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	93.5
2	95.0
3	494.5
4	894.0
5	894.0
6	1188.0
7	1482.0
8	1472.0
9	1462.0
10	2183.5
11	2905.0
12	2905.0
13	4672.0
14	6439.0
15	10849.5
16	15260.0
17	13815.0
18	12370.0
19	12370.0
20	13338.0
21	14306.0
22	11420.0
23	8534.0
24	9249.5
25	9965.0
26	9965.0
27	11036.0
28	12107.0
29	14237.5
30	16368.0
31	18603.0
32	20838.0
33	20838.0
34	24398.5
35	27959.0
36	32026.5
37	36094.0
38	39654.5
39	43215.0
40	43215.0
41	46199.0
42	49183.0
43	51307.5
44	53432.0
45	57558.5
46	61685.0
47	61685.0
48	68046.0
49	74407.0
50	72355.5
51	70304.0
52	68602.0
53	66900.0
54	66900.0
55	62717.5
56	58535.0
57	56473.0
58	54411.0
59	53488.5
60	52566.0
61	52566.0
62	48747.0
63	44928.0
64	42324.0
65	39720.0
66	35388.0
67	31056.0
68	31056.0
69	26372.0
70	21688.0
71	18423.0
72	15158.0
73	10718.5
74	6279.0
75	6279.0
76	4920.0
77	3561.0
78	2913.0
79	2265.0
80	2092.5
81	1920.0
82	1920.0
83	1602.0
84	1284.0
85	1188.0
86	1092.0
87	951.5
88	811.0
89	811.0
90	562.0
91	313.0
92	183.0
93	53.0
94	32.5
95	12.0
96	12.0
97	12.0
98	12.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	941972.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.76680831417562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.48131673327285	48.64891612812093
2	4.854708197661733	5.220443301698708
3	1.3755029511535293	2.2186921053076403
4	0.7041860155101751	1.5144733805383475
5	0.4551548941348556	1.2236112973103839
6	0.29836927932939966	0.9625418309125533
7	0.22218822369142388	0.8362446143018774
8	0.1788083434336787	0.769116314109911
9	0.13608324024243873	0.6585085343598402
>10	1.0020850203609197	10.731241444567324
>50	0.14570096060972484	5.447021868674071
>100	0.1310692970513781	13.883684249372791
>500	0.012256857332992028	4.34746029859152
>1k	0.0019769124730632305	1.4385596362225286
>5k	5.93073741918969E-4	2.0994849959115847
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8124	0.862446017503705	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5871	0.623266933624354	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5757	0.6111646630685413	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1961	0.20818028561358512	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1718	0.18238334048145805	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1678	0.17813692976012027	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1549	0.1644422551838059	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1164	0.12357055199092967	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1149	0.12197814797042801	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1126	0.11953646180565877	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1087	0.11539621135235442	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1072	0.11380380733185275	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1030	0.10934507607444809	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	949	0.10074609436373905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	8.492821442675579E-4	0.0
12	0.0	0.0	0.0	0.0014862437524682263	0.0
13	0.0	0.0	0.0	0.0014862437524682263	0.0
14	0.0	0.0	0.0	0.0018047245565685605	0.0
15	0.0	0.0	0.0	0.0025478464328026734	0.0
16	0.0	0.0	0.0	0.004246410721337789	0.0
17	0.0	0.0	0.0	0.006369616082006684	0.0
18	1.0616026803344474E-4	0.0	0.0	0.007006577690207352	0.0
19	1.0616026803344474E-4	0.0	0.0	0.008811302246775912	0.0
20	2.1232053606688947E-4	0.0	0.0	0.00987290492711036	0.0
21	2.1232053606688947E-4	0.0	0.0	0.012633071895979923	0.0
22	2.1232053606688947E-4	0.0	0.0	0.018047245565685606	0.0
23	2.1232053606688947E-4	0.0	0.0	0.024841502719826068	0.0
24	2.1232053606688947E-4	0.0	0.0	0.03598833086333776	0.0
25	2.1232053606688947E-4	0.0	0.0	0.04108402372894311	0.0
26	2.1232053606688947E-4	0.0	0.0	0.04809060141915046	0.0
27	2.1232053606688947E-4	0.0	0.0	0.08259268853002	0.0
28	2.1232053606688947E-4	0.0	0.0	0.2268644927874714	0.0
29	2.1232053606688947E-4	0.0	0.0	0.4727316735529294	0.0
30	2.1232053606688947E-4	0.0	0.0	0.824971442887899	0.0
31	2.1232053606688947E-4	0.0	0.0	1.6473950393429952	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGGT	1035	0.0	22.52174	11
GCAGTCG	1075	0.0	22.199999	9
CAGTCGG	1070	0.0	21.957943	10
GGCAGTC	1105	0.0	21.597286	8
TCTATCG	60	3.725369E-5	21.583332	31
TCGGTGA	1085	0.0	21.313364	13
GGGCAGT	1155	0.0	20.982683	7
GTCGGTG	1115	0.0	20.739912	12
TATTCGT	45	0.0038253025	20.555557	6
CGGTGAT	1125	0.0	20.555555	14
TTCGTAC	55	5.142145E-4	20.18182	8
AGTCTGC	55	5.142145E-4	20.18182	8
GGTGATT	1180	0.0	19.754238	15
GATTCCT	1145	0.0	19.550217	18
CGCCTTA	805	0.0	19.534163	25
ATTCCTC	1170	0.0	19.290598	19
GGTATCA	3185	0.0	19.284145	1
GCCTTAT	825	0.0	19.060606	26
TTCCTCG	835	0.0	19.053892	20
GTGATTC	1195	0.0	19.041842	16
>>END_MODULE