FastQCFastQC Report
Fri 10 Feb 2017
ERR1631397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551545
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.5005937865450689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24980.45290955407083733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21790.39507202494810034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.17822661795501726No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA7910.14341531516014105No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6730.12202086865079007No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6280.11386196955824093No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5900.10697223254675502No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC5850.10606568820313847No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5580.101170348747609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAC250.005493894729.67
GTCTAAT405.9346115E-527.7500021
GGTATCA12300.021.056911
CAGTCGG2050.020.75609610
AGAGCGA1700.020.67647215
CTGTGCG450.00382332320.5555559
AGTCGGT2100.020.26190411
TAGTCCA656.893813E-519.92307729
GCAGTCG2250.019.7333349
GTCCTAT1700.019.5882361
AAGACGG1900.018.55
CGGTGAT2300.018.514
GCAGCTA1601.8189894E-1218.532
AACTAGC500.00703035518.537
CGCTTAT801.6141865E-518.526
TCGGTGA2250.018.08888813
GTCGGTG2250.018.08888812
ACGGACC2150.018.0697678
CTCTATG3800.018.0131591
CAGCTAG1750.017.9714333
Produced by FastQC (version 0.11.5)