##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631397.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551545
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03076448884497	34.0	33.0	34.0	31.0	34.0
2	33.15930522441505	34.0	33.0	34.0	31.0	34.0
3	33.2375907677524	34.0	33.0	34.0	31.0	34.0
4	36.477846775875044	37.0	37.0	37.0	35.0	37.0
5	36.46713142173349	37.0	37.0	37.0	35.0	37.0
6	36.52272616015012	37.0	37.0	37.0	35.0	37.0
7	36.534998957474	37.0	37.0	37.0	35.0	37.0
8	36.54026416702173	37.0	37.0	37.0	35.0	37.0
9	38.37291245501274	39.0	39.0	39.0	37.0	39.0
10	38.349014133026316	39.0	39.0	39.0	37.0	39.0
11	38.392720448920755	39.0	39.0	39.0	37.0	39.0
12	38.35979113218323	39.0	39.0	39.0	37.0	39.0
13	38.3861244322766	39.0	39.0	39.0	37.0	39.0
14	39.91173159035074	41.0	40.0	41.0	38.0	41.0
15	39.89462328549801	41.0	40.0	41.0	38.0	41.0
16	39.84672873473606	41.0	40.0	41.0	38.0	41.0
17	39.8537816497294	41.0	40.0	41.0	38.0	41.0
18	39.835681585364746	41.0	40.0	41.0	38.0	41.0
19	39.85334832153315	41.0	40.0	41.0	38.0	41.0
20	39.816555312803125	41.0	40.0	41.0	38.0	41.0
21	39.78876247631653	41.0	40.0	41.0	38.0	41.0
22	39.7165942942099	41.0	40.0	41.0	37.0	41.0
23	39.646857464032855	41.0	40.0	41.0	37.0	41.0
24	39.61198814239999	41.0	40.0	41.0	37.0	41.0
25	39.60897660209049	41.0	40.0	41.0	37.0	41.0
26	39.50636303474784	41.0	40.0	41.0	37.0	41.0
27	39.44563725534635	41.0	39.0	41.0	36.0	41.0
28	39.35992711383477	41.0	39.0	41.0	36.0	41.0
29	39.29082849087563	41.0	39.0	41.0	35.0	41.0
30	39.21712099647354	41.0	39.0	41.0	35.0	41.0
31	39.134895611418834	41.0	39.0	41.0	35.0	41.0
32	39.04975296666636	41.0	39.0	41.0	35.0	41.0
33	38.94423845742414	41.0	39.0	41.0	35.0	41.0
34	38.87404291580923	41.0	39.0	41.0	35.0	41.0
35	38.765542249499134	40.0	38.0	41.0	35.0	41.0
36	38.68642812463172	40.0	38.0	41.0	35.0	41.0
37	38.59996736440363	40.0	38.0	41.0	35.0	41.0
38	38.50816161872558	40.0	38.0	41.0	35.0	41.0
39	38.39574286776238	40.0	38.0	41.0	35.0	41.0
40	38.265992802037914	40.0	38.0	41.0	35.0	41.0
41	38.14658278109674	40.0	37.0	41.0	35.0	41.0
42	37.988370849160084	40.0	37.0	41.0	34.0	41.0
43	37.199417998531395	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	2.0
14	2.0
15	1.0
16	5.0
17	3.0
18	12.0
19	16.0
20	47.0
21	85.0
22	148.0
23	229.0
24	381.0
25	584.0
26	922.0
27	1298.0
28	1979.0
29	2612.0
30	3427.0
31	4353.0
32	5440.0
33	7298.0
34	10661.0
35	15548.0
36	24721.0
37	49375.0
38	101957.0
39	320436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.390838462863414	18.757127704901684	12.737310645550226	25.114723186684678
2	19.656782311506767	20.838009591239157	32.965759820141606	26.539448277112477
3	20.371683180882794	22.531615733983628	29.335593650563418	27.761107434570164
4	14.506703895421044	16.109292986066414	33.92724075098133	35.456762367531205
5	15.83170910805102	35.33963683833595	32.08496133588375	16.74369271772929
6	33.33399813251865	34.204824628996725	16.380168435939044	16.081008802545576
7	29.335956268300862	30.440127278825845	20.693506422866676	19.530410030006617
8	28.750147313455837	30.485635804875393	20.094824538342294	20.66939234332647
9	27.364584938672277	14.244531271247132	20.40867019010235	37.982213599978245
10	19.48562673943196	25.371637853665614	30.054483315051357	25.08825209185107
11	36.44779664397284	21.79314471167357	20.358628942334715	21.400429702018876
12	23.15006028519885	26.14909028275118	26.767534833966405	23.933314598083566
13	30.814530092739485	18.72993137459319	24.902773119147124	25.552765413520202
14	23.68619061001369	21.26009663762703	24.171191833848553	30.882520918510732
15	26.895538895285064	26.675792546392408	21.636675157965353	24.79199340035718
16	25.679137695020355	25.430200618263243	23.81582645115086	25.074835235565544
17	24.45058880055118	26.112103273531623	24.389125094053977	25.04818283186322
18	24.659456617320437	23.476960175506985	26.611427897995632	25.25215530917695
19	24.87412631788884	25.013371529068344	26.106120080863754	24.00638207217906
20	25.472445584675775	23.54404445693461	25.96995712045255	25.01355283793707
21	27.234405170928937	24.132391735941763	25.159325168390613	23.47387792473869
22	26.643156950022213	23.93530899563952	24.960791957138582	24.460742097199685
23	25.111459627047655	23.728072958688777	25.595735615407627	25.564731798855938
24	24.275263124495737	25.08480722334533	25.702707847954382	24.937221804204555
25	24.65093510049044	24.031765313800324	25.63199738915229	25.685302196556947
26	24.645133216691296	25.11164093591638	25.70977889383459	24.533446953557732
27	25.385236018819864	23.876020995567	25.47317082015067	25.265572165462473
28	24.095948653328378	24.464005656836704	26.53437162878822	24.905674061046696
29	23.59807449981416	24.802146697005682	26.896264130759956	24.7035146724202
30	23.53624817557951	25.31488817775521	26.77279279115938	24.376070855505898
31	24.480504763890526	25.07120905819108	25.427480985232393	25.020805192686
32	23.651016689481366	24.515316066685404	25.97340198895829	25.860265254874943
33	23.216600640020307	24.23229292260831	26.790561060294266	25.760545377077122
34	24.554297473460913	23.92261737482889	26.603631616640527	24.919453535069668
35	24.192948898095352	23.83069377838617	27.246734174002125	24.729623149516357
36	22.836758560044963	24.49609732660073	27.02408688320989	25.64305723014441
37	23.53733602879185	23.218232419838817	27.75403638868995	25.49039516267938
38	22.765866792374148	22.495535269107688	28.843883998585788	25.894713939932373
39	23.022056223880192	22.154493287039134	29.136516512705217	25.686933976375453
40	22.10608381909001	22.55464196031149	29.81823785910488	25.52103636149362
41	21.13046079648986	22.645659012410594	30.911711646375185	25.31216854472437
42	21.773925971588902	22.8385716487322	30.571213590912798	24.8162887887661
43	20.335421407138128	22.158663391019772	30.836831083592454	26.669084118249643
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	270.0
1	220.0
2	170.0
3	350.5
4	531.0
5	531.0
6	665.0
7	799.0
8	774.5
9	750.0
10	1102.5
11	1455.0
12	1455.0
13	2379.0
14	3303.0
15	4672.0
16	6041.0
17	5648.5
18	5256.0
19	5256.0
20	5862.5
21	6469.0
22	5602.5
23	4736.0
24	5234.0
25	5732.0
26	5732.0
27	6449.5
28	7167.0
29	8316.5
30	9466.0
31	10833.5
32	12201.0
33	12201.0
34	14322.5
35	16444.0
36	19277.5
37	22111.0
38	23792.0
39	25473.0
40	25473.0
41	27040.0
42	28607.0
43	29884.5
44	31162.0
45	32938.0
46	34714.0
47	34714.0
48	36750.5
49	38787.0
50	38800.0
51	38813.0
52	39121.0
53	39429.0
54	39429.0
55	37943.0
56	36457.0
57	35312.0
58	34167.0
59	33121.0
60	32075.0
61	32075.0
62	29834.0
63	27593.0
64	25431.5
65	23270.0
66	20771.0
67	18272.0
68	18272.0
69	15868.0
70	13464.0
71	11776.0
72	10088.0
73	7573.5
74	5059.0
75	5059.0
76	4110.0
77	3161.0
78	2740.5
79	2320.0
80	2165.5
81	2011.0
82	2011.0
83	1652.0
84	1293.0
85	1186.5
86	1080.0
87	975.5
88	871.0
89	871.0
90	631.0
91	391.0
92	222.0
93	53.0
94	37.5
95	22.0
96	22.0
97	16.0
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	551545.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.12508244750636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.72410714818658	54.547944222611186
2	4.642029807280493	5.582048497730435
3	1.3714287492339872	2.4737179985572206
4	0.70339537973322	1.6916682079861955
5	0.45154863462592937	1.3574699442971465
6	0.30130054196834094	1.086943195639491
7	0.22914529260705815	0.964416572732014
8	0.17850662136109416	0.858618026140927
9	0.14088551589862663	0.7623677933157956
>10	1.0362617966726244	12.537855257471357
>50	0.12309554525745928	5.244436926371245
>100	0.0943631684876051	10.388131090304062
>500	0.0030244605284488794	1.1518121752964658
>1k	9.073381585346637E-4	1.352570091546434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2761	0.5005937865450689	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2498	0.45290955407083733	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2179	0.39507202494810034	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	983	0.17822661795501726	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	791	0.14341531516014105	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	673	0.12202086865079007	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	628	0.11386196955824093	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	590	0.10697223254675502	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	585	0.10606568820313847	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	558	0.101170348747609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.813088687233136E-4	0.0
3	0.0	0.0	0.0	1.813088687233136E-4	0.0
4	0.0	0.0	0.0	1.813088687233136E-4	0.0
5	0.0	0.0	0.0	1.813088687233136E-4	0.0
6	0.0	0.0	0.0	1.813088687233136E-4	0.0
7	0.0	0.0	0.0	1.813088687233136E-4	0.0
8	0.0	0.0	0.0	1.813088687233136E-4	0.0
9	0.0	0.0	0.0	1.813088687233136E-4	0.0
10	0.0	0.0	0.0	1.813088687233136E-4	0.0
11	0.0	0.0	0.0	1.813088687233136E-4	0.0
12	0.0	0.0	0.0	5.439266061699408E-4	0.0
13	0.0	0.0	0.0	5.439266061699408E-4	0.0
14	0.0	0.0	0.0	5.439266061699408E-4	0.0
15	0.0	0.0	0.0	9.06544343616568E-4	0.0
16	0.0	0.0	0.0	0.002175706424679763	0.0
17	0.0	0.0	0.0	0.003263559637019645	0.0
18	0.0	0.0	0.0	0.003988795111912899	0.0
19	0.0	0.0	0.0	0.004895339455529467	0.0
20	0.0	0.0	0.0	0.005801883799146035	0.0
21	0.0	0.0	0.0	0.007433663617655858	0.0
22	0.0	0.0	0.0	0.010153296648505561	0.0
23	0.0	0.0	0.0	0.01305423854807858	0.0
24	0.0	0.0	0.0	0.01722434252871479	0.0
25	0.0	0.0	0.0	0.0226636085904142	0.0
26	0.0	0.0	0.0	0.03825617130061917	0.0
27	0.0	0.0	0.0	0.09355537626122981	0.0
28	0.0	0.0	0.0	0.2904568076947484	0.0
29	0.0	0.0	0.0	0.5377621046333482	0.0
30	0.0	0.0	0.0	0.8760844536710514	0.0
31	0.0	0.0	0.0	1.6172751090119573	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTTAC	25	0.0054938947	29.6	7
GTCTAAT	40	5.9346115E-5	27.750002	1
GGTATCA	1230	0.0	21.05691	1
CAGTCGG	205	0.0	20.756096	10
AGAGCGA	170	0.0	20.676472	15
CTGTGCG	45	0.003823323	20.555555	9
AGTCGGT	210	0.0	20.261904	11
TAGTCCA	65	6.893813E-5	19.923077	29
GCAGTCG	225	0.0	19.733334	9
GTCCTAT	170	0.0	19.588236	1
AAGACGG	190	0.0	18.5	5
CGGTGAT	230	0.0	18.5	14
GCAGCTA	160	1.8189894E-12	18.5	32
AACTAGC	50	0.007030355	18.5	37
CGCTTAT	80	1.6141865E-5	18.5	26
TCGGTGA	225	0.0	18.088888	13
GTCGGTG	225	0.0	18.088888	12
ACGGACC	215	0.0	18.069767	8
CTCTATG	380	0.0	18.013159	1
CAGCTAG	175	0.0	17.97143	33
>>END_MODULE