FastQCFastQC Report
Fri 10 Feb 2017
ERR1631396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130597
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13331.0206972595082582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10770.8246743799627863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10200.7810286606889898No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA8520.6523886459872739No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT7770.5949600679954364No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT7650.5857714955167423No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC6870.5260457744052314No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC6790.5199200594194353No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT6580.5038400575817208No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT6270.48010291201176136No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA5060.38745147285159687No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA4980.3813257578658009No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT4360.33385146672588195No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT3790.2902057474520854No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA3650.2794857462269424No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT3630.27795431748049343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.26264003001600345No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA3390.2595771725231054No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA3290.2519200287908604No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA3190.24426288505861546No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT3070.23507431257992145No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT3070.23507431257992145No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA2980.2281828832209009No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA2950.22588574010122744No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC2880.22052573948865595No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA2830.21669716762253347No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC2650.20291430890449244No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT2540.19449145079902297No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG2400.18377144957387995No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA2250.17228573397551245No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT2180.16692573336294098No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT2170.16616001898971644No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT2080.15926858963069596No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT2060.15773716088424697No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA2010.15390858901812446No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT1920.14701715965910395No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT1780.13629715843396095No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC1760.13476572968751196No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1730.13246858656783847No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.13170287219461396No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT1650.12634287158204247No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT1640.12557715720881796No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT1580.12098287096947097No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT1570.12021715659624647No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT1550.11868572784979746No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1530.11715429910334847No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT1530.11715429910334847No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC1500.11485715598367498ABI Solid3 Adapter B (100% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC1470.11256001286400147No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT1470.11256001286400147No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT1450.11102858411755248No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA1400.10720001225142997ABI Solid3 Adapter B (100% over 21bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCT1390.10643429787820548No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA1380.10566858350498098No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1310.10030858289240946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAA250.005478735529.62
CTCCCAT250.005478735529.67
TGCTGTG250.005478735529.631
TTGAACC250.005478735529.64
TATTGAC1450.028.06896630
ATGCCCC1450.028.06896637
ATTGACT1400.027.7531
AGACTGT358.827175E-426.4285734
TGAACCA358.827175E-426.428575
CCTATGG509.010759E-625.9000021
ACCGACC959.094947E-1225.3157932
TTGACTG1550.025.06451632
TATGCTG451.3144559E-424.66666636
TGACTGT1700.023.94117733
GCAGTCG10850.021.6543799
CAGTCGG10750.021.51162710
GGCAGTC10950.021.4566238
AGTCGGT10650.021.36619811
GGGCAGT11100.021.3333327
TGGGCAG11250.021.2133336
Produced by FastQC (version 0.11.5)