##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631396.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130597
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.873029242631915	34.0	31.0	34.0	31.0	34.0
2	32.96952456794566	34.0	33.0	34.0	31.0	34.0
3	32.927280105974866	34.0	33.0	34.0	31.0	34.0
4	36.343063010635774	37.0	37.0	37.0	35.0	37.0
5	36.346493411027815	37.0	37.0	37.0	35.0	37.0
6	36.42549216291339	37.0	37.0	37.0	35.0	37.0
7	36.45001799428777	37.0	37.0	37.0	35.0	37.0
8	36.46962793938605	37.0	37.0	37.0	35.0	37.0
9	38.25799980091426	39.0	39.0	39.0	37.0	39.0
10	38.21965282510318	39.0	39.0	39.0	37.0	39.0
11	38.272288031118634	39.0	39.0	39.0	37.0	39.0
12	38.24306837063639	39.0	39.0	39.0	37.0	39.0
13	38.24730277112032	39.0	39.0	39.0	37.0	39.0
14	39.61540464176053	41.0	39.0	41.0	37.0	41.0
15	39.56089343553068	41.0	39.0	41.0	37.0	41.0
16	39.40485616055499	40.0	39.0	41.0	37.0	41.0
17	39.38382198672251	40.0	39.0	41.0	37.0	41.0
18	39.57260120829728	40.0	39.0	41.0	37.0	41.0
19	39.613161098646984	41.0	39.0	41.0	37.0	41.0
20	39.673621905556786	41.0	40.0	41.0	38.0	41.0
21	39.684801335405865	41.0	40.0	41.0	37.0	41.0
22	39.62644624302243	41.0	40.0	41.0	37.0	41.0
23	39.56274646431388	41.0	40.0	41.0	37.0	41.0
24	39.511152629846016	41.0	39.0	41.0	37.0	41.0
25	39.500072742865456	41.0	39.0	41.0	37.0	41.0
26	39.392428616277556	41.0	39.0	41.0	36.0	41.0
27	39.303360720384084	41.0	39.0	41.0	36.0	41.0
28	39.19302127920243	41.0	39.0	41.0	35.0	41.0
29	39.0701241222999	40.0	39.0	41.0	35.0	41.0
30	38.92019724802254	40.0	38.0	41.0	35.0	41.0
31	38.75256705743624	40.0	38.0	41.0	35.0	41.0
32	38.60192806879178	40.0	38.0	41.0	35.0	41.0
33	38.48707091280811	40.0	38.0	41.0	35.0	41.0
34	38.39360781641232	40.0	38.0	41.0	35.0	41.0
35	38.26884231643912	40.0	37.0	41.0	35.0	41.0
36	38.18603030697489	40.0	37.0	41.0	35.0	41.0
37	38.07147177959678	40.0	37.0	41.0	35.0	41.0
38	37.95069565150808	40.0	37.0	41.0	34.0	41.0
39	37.765193687450704	40.0	36.0	41.0	34.0	41.0
40	37.61692841336325	40.0	36.0	41.0	33.0	41.0
41	37.40193113164927	40.0	35.0	41.0	33.0	41.0
42	37.22383362558099	40.0	35.0	41.0	33.0	41.0
43	36.37163947104451	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	6.0
19	8.0
20	7.0
21	18.0
22	40.0
23	59.0
24	108.0
25	165.0
26	242.0
27	360.0
28	571.0
29	803.0
30	1062.0
31	1324.0
32	1518.0
33	2006.0
34	2733.0
35	4341.0
36	7277.0
37	16229.0
38	31656.0
39	60060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36393638444987	12.3984471312511	17.188756250143573	27.048860234155452
2	21.162813847178725	18.495830685237795	34.83311255235572	25.508242915227765
3	18.436104964126283	21.892539644861674	31.01908925932449	28.652266131687558
4	12.85251575457323	20.19571659379618	31.99307794206605	34.95868970956454
5	14.293590204981738	37.46180999563543	31.06579783609118	17.178801963291654
6	27.38960313024036	30.81847209353967	22.088562524407145	19.703362251812827
7	30.68294064947893	31.524460745652654	16.762253344257523	21.030345260610886
8	34.87905541474919	28.504483257655227	16.59456189652136	20.021899431074218
9	31.83304363806213	12.69784145118188	14.900801702948765	40.56831320780722
10	21.917042504804858	24.384174215334195	20.79450523365774	32.9042780462032
11	33.70215242310313	26.701225908711535	13.399235817055521	26.197385851129813
12	25.90488296055805	29.992266284830432	22.587042581376295	21.51580817323522
13	36.582004180800475	17.900870617242358	23.541888404787244	21.97523679716992
14	24.867339984838853	21.150562417207134	22.7715797453234	31.21051785263061
15	35.8201183794421	21.60386532615604	18.53258497515257	24.043431319249294
16	25.464597195953964	24.98908857018155	24.583259952372565	24.963054281491917
17	19.146687902478618	30.45935205249738	24.740231398883587	25.653728646140415
18	25.668277219231683	20.39480233083455	26.977648797445575	26.959271652488187
19	24.429351363354442	30.028254860371984	26.152208703109565	19.39018507316401
20	28.238014655773103	19.75313368607242	29.684449106794187	22.324402551360294
21	34.26342105867669	17.529499146228474	25.20502002343086	23.002059771663973
22	31.568872179299678	19.527247945971194	20.498939485593084	28.404940389136048
23	25.899522959945482	23.170516933773364	21.604631040529263	29.325329065751895
24	24.116939899078847	23.123042642633447	28.578757551858008	24.181259906429702
25	22.747076885380217	22.246299685291394	24.630734243512485	30.375889185815907
26	29.868220556368065	19.926950848794384	22.73941974164797	27.465408853189583
27	25.776242945856335	20.29066517607602	22.694242593627724	31.23884928443992
28	23.50819697236537	20.768470944968108	27.54963743424428	28.17369464842224
29	23.839751295971574	21.547968176910647	30.460883481243826	24.15139704587395
30	23.167454076280467	22.90404833189124	31.438700735851512	22.489796855976785
31	23.62075698522937	24.50362565755722	25.694311507921313	26.181305849292098
32	21.384105301040606	23.979111311898436	31.948666508419027	22.688116878641928
33	24.462277081403094	20.44074519322802	31.995375085185724	23.10160264018316
34	25.775477231483112	23.106196926422506	24.3030084917724	26.815317350321983
35	22.702665451733196	24.013568458693538	26.997557371149416	26.286208718423854
36	18.96291645290474	22.944631193672134	29.119351899354502	28.97310045406862
37	20.608436640964186	22.881842615067725	30.405752046371664	26.103968697596425
38	21.34045958176681	17.703316308950434	32.483900855302956	28.4723232539798
39	21.06327097865954	17.79213917624448	35.53450691822936	25.610082926866617
40	20.60077949723194	17.609133441043824	31.636255044143436	30.1538320175808
41	15.317350321982895	20.82972809482607	36.574347037068236	27.278574546122808
42	16.081533266460944	19.285282204032253	34.40660964646967	30.226574883037127
43	13.91456159023561	23.151374074442753	37.41510141886874	25.518962916452903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	3.5
2	7.0
3	50.0
4	93.0
5	93.0
6	148.5
7	204.0
8	217.5
9	231.0
10	330.0
11	429.0
12	429.0
13	785.5
14	1142.0
15	1847.0
16	2552.0
17	2292.5
18	2033.0
19	2033.0
20	2175.5
21	2318.0
22	1604.5
23	891.0
24	768.0
25	645.0
26	645.0
27	596.0
28	547.0
29	564.5
30	582.0
31	586.0
32	590.0
33	590.0
34	627.0
35	664.0
36	746.5
37	829.0
38	1186.5
39	1544.0
40	1544.0
41	2436.5
42	3329.0
43	4887.5
44	6446.0
45	9225.5
46	12005.0
47	12005.0
48	16497.5
49	20990.0
50	19828.5
51	18667.0
52	14622.0
53	10577.0
54	10577.0
55	8287.0
56	5997.0
57	5872.0
58	5747.0
59	5613.0
60	5479.0
61	5479.0
62	5471.0
63	5463.0
64	5275.5
65	5088.0
66	4870.5
67	4653.0
68	4653.0
69	4206.5
70	3760.0
71	3312.5
72	2865.0
73	2407.0
74	1949.0
75	1949.0
76	1545.0
77	1141.0
78	890.5
79	640.0
80	479.5
81	319.0
82	319.0
83	221.0
84	123.0
85	81.5
86	40.0
87	27.0
88	14.0
89	14.0
90	8.5
91	3.0
92	1.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	130597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.08249806657121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.02689376961004	52.28986883312786
2	5.039945157803148	5.854652097674525
3	1.5055237693463759	2.623337442667136
4	0.7830832915864687	1.8193373507814117
5	0.5391937142405147	1.5658858932441022
6	0.36253856091966147	1.2634287158204245
7	0.2293880349091676	0.9326401065874408
8	0.17797347536056107	0.8269715230824598
9	0.14369710232815672	0.7511658001332343
>10	0.9795132755029399	11.298881291300718
>50	0.11996730561341525	4.685406249760715
>100	0.07778100034276372	9.211543909890732
>500	0.010546576317662879	4.250480485769199
>1k	0.003954966119123579	2.6264003001600345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1333	1.0206972595082582	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1077	0.8246743799627863	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1020	0.7810286606889898	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	852	0.6523886459872739	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	777	0.5949600679954364	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	765	0.5857714955167423	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	687	0.5260457744052314	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	679	0.5199200594194353	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	658	0.5038400575817208	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	627	0.48010291201176136	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	506	0.38745147285159687	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	498	0.3813257578658009	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	436	0.33385146672588195	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	379	0.2902057474520854	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	365	0.2794857462269424	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	363	0.27795431748049343	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.26264003001600345	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	339	0.2595771725231054	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	329	0.2519200287908604	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	319	0.24426288505861546	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	307	0.23507431257992145	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	307	0.23507431257992145	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	298	0.2281828832209009	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	295	0.22588574010122744	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	288	0.22052573948865595	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	283	0.21669716762253347	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	265	0.20291430890449244	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	254	0.19449145079902297	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	240	0.18377144957387995	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	225	0.17228573397551245	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	218	0.16692573336294098	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	217	0.16616001898971644	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	208	0.15926858963069596	No Hit
GTCGGTGATTCCTCCGCTTATTGATATGCCCATAGAGCTGTCT	206	0.15773716088424697	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	201	0.15390858901812446	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT	192	0.14701715965910395	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	178	0.13629715843396095	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC	176	0.13476572968751196	No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	173	0.13246858656783847	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	172	0.13170287219461396	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	165	0.12634287158204247	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	164	0.12557715720881796	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	158	0.12098287096947097	No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT	157	0.12021715659624647	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT	155	0.11868572784979746	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	153	0.11715429910334847	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	153	0.11715429910334847	No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC	150	0.11485715598367498	ABI Solid3 Adapter B (100% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC	147	0.11256001286400147	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	147	0.11256001286400147	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGACTGTCTCT	145	0.11102858411755248	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	140	0.10720001225142997	ABI Solid3 Adapter B (100% over 21bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCT	139	0.10643429787820548	No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA	138	0.10566858350498098	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	131	0.10030858289240946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	7.657143732244998E-4	0.0	0.0	0.0	0.0
11	7.657143732244998E-4	0.0	0.0	7.657143732244998E-4	0.0
12	7.657143732244998E-4	0.0	0.0	7.657143732244998E-4	0.0
13	7.657143732244998E-4	0.0	0.0	7.657143732244998E-4	0.0
14	7.657143732244998E-4	0.0	0.0	0.0015314287464489997	0.0
15	7.657143732244998E-4	0.0	0.0	0.0030628574928979993	0.0
16	7.657143732244998E-4	0.0	0.0	0.016080001837714495	7.657143732244998E-4
17	7.657143732244998E-4	0.0	0.0	0.02986286055575549	7.657143732244998E-4
18	7.657143732244998E-4	0.0	0.0	0.03369143242187799	7.657143732244998E-4
19	7.657143732244998E-4	0.0	0.0	0.04288000490057199	7.657143732244998E-4
20	7.657143732244998E-4	0.0	0.0	0.05589714924538849	7.657143732244998E-4
21	7.657143732244998E-4	0.0	0.0	0.06968000796342948	7.657143732244998E-4
22	7.657143732244998E-4	0.0	0.0	0.09571429665306247	7.657143732244998E-4
23	7.657143732244998E-4	0.0	0.0	0.13629715843396095	7.657143732244998E-4
24	7.657143732244998E-4	0.0	0.0	0.21593145324930893	7.657143732244998E-4
25	7.657143732244998E-4	0.0	0.0	0.24043431319249295	7.657143732244998E-4
26	7.657143732244998E-4	0.0	0.0	0.2764228887340444	7.657143732244998E-4
27	7.657143732244998E-4	0.0	0.0	0.3583543266690659	7.657143732244998E-4
28	7.657143732244998E-4	0.0	0.0	0.6539200747337228	7.657143732244998E-4
29	7.657143732244998E-4	0.0	0.0	1.199874422842791	7.657143732244998E-4
30	7.657143732244998E-4	0.0	0.0	2.1455316737750483	7.657143732244998E-4
31	7.657143732244998E-4	0.0	0.0	4.251246200142423	7.657143732244998E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAA	25	0.0054787355	29.6	2
CTCCCAT	25	0.0054787355	29.6	7
TGCTGTG	25	0.0054787355	29.6	31
TTGAACC	25	0.0054787355	29.6	4
TATTGAC	145	0.0	28.068966	30
ATGCCCC	145	0.0	28.068966	37
ATTGACT	140	0.0	27.75	31
AGACTGT	35	8.827175E-4	26.42857	34
TGAACCA	35	8.827175E-4	26.42857	5
CCTATGG	50	9.010759E-6	25.900002	1
ACCGACC	95	9.094947E-12	25.31579	32
TTGACTG	155	0.0	25.064516	32
TATGCTG	45	1.3144559E-4	24.666666	36
TGACTGT	170	0.0	23.941177	33
GCAGTCG	1085	0.0	21.654379	9
CAGTCGG	1075	0.0	21.511627	10
GGCAGTC	1095	0.0	21.456623	8
AGTCGGT	1065	0.0	21.366198	11
GGGCAGT	1110	0.0	21.333332	7
TGGGCAG	1125	0.0	21.213333	6
>>END_MODULE