FastQCFastQC Report
Fri 10 Feb 2017
ERR1631395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794464
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63260.3525286659414733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50700.28253562066444354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46440.2587959412950051No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.11847548905968579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.019.8158991
ACCGTCC951.6774175E-719.4736848
TATACAC8000.017.3437537
TATATAG752.068081E-417.2666665
CAAGACG4750.016.3578954
AAGACGG5000.016.2799995
AGTCGGT4850.016.02061811
ACGGACC4750.015.9684218
GTGTAAC1059.348862E-615.8571441
ATACACA6100.015.77049337
GCAGTCG5000.015.549
TCTAGAC3150.015.2698423
TTATACA13750.015.06909137
TACCGTC2950.015.0508477
TCGTTTA3200.015.03125130
ATACCGT3600.014.9027786
CGTTTAT3300.014.57575731
CCCTAGT908.2772056E-414.3888881
AAGACTA908.2772056E-414.3888885
GTTCTAG3100.014.3225811
Produced by FastQC (version 0.11.5)