##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631395.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1794464
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05795936836849	34.0	33.0	34.0	31.0	34.0
2	33.18588280400164	34.0	33.0	34.0	31.0	34.0
3	33.26812797581896	34.0	34.0	34.0	31.0	34.0
4	36.510561370972056	37.0	37.0	37.0	35.0	37.0
5	36.48891423845784	37.0	37.0	37.0	35.0	37.0
6	36.53740281220465	37.0	37.0	37.0	35.0	37.0
7	36.54678277190292	37.0	37.0	37.0	35.0	37.0
8	36.54670363963835	37.0	37.0	37.0	35.0	37.0
9	38.39684942133138	39.0	39.0	39.0	37.0	39.0
10	38.3550848609947	39.0	39.0	39.0	37.0	39.0
11	38.413767565133654	39.0	39.0	39.0	37.0	39.0
12	38.37591726554559	39.0	39.0	39.0	37.0	39.0
13	38.39947806141555	39.0	39.0	39.0	37.0	39.0
14	39.93657716175972	41.0	40.0	41.0	38.0	41.0
15	39.92129404657881	41.0	40.0	41.0	38.0	41.0
16	39.89045252509942	41.0	40.0	41.0	38.0	41.0
17	39.890633637676764	41.0	40.0	41.0	38.0	41.0
18	39.8703897096849	41.0	40.0	41.0	38.0	41.0
19	39.877749567558894	41.0	40.0	41.0	38.0	41.0
20	39.839032156677426	41.0	40.0	41.0	38.0	41.0
21	39.80829094370241	41.0	40.0	41.0	38.0	41.0
22	39.754487691031976	41.0	40.0	41.0	38.0	41.0
23	39.69350680760383	41.0	40.0	41.0	37.0	41.0
24	39.6577986518537	41.0	40.0	41.0	37.0	41.0
25	39.65560914011092	41.0	40.0	41.0	37.0	41.0
26	39.55924164541612	41.0	40.0	41.0	37.0	41.0
27	39.50596166877686	41.0	40.0	41.0	37.0	41.0
28	39.44268985056262	41.0	39.0	41.0	36.0	41.0
29	39.39003958842306	41.0	39.0	41.0	36.0	41.0
30	39.31947812828789	41.0	39.0	41.0	36.0	41.0
31	39.26332877115395	41.0	39.0	41.0	35.0	41.0
32	39.177918866023504	41.0	39.0	41.0	35.0	41.0
33	39.09006979242827	41.0	39.0	41.0	35.0	41.0
34	39.029545869964515	41.0	39.0	41.0	35.0	41.0
35	38.93530881644881	41.0	39.0	41.0	35.0	41.0
36	38.86382953349858	41.0	39.0	41.0	35.0	41.0
37	38.79213570180288	40.0	38.0	41.0	35.0	41.0
38	38.708861253276744	40.0	38.0	41.0	35.0	41.0
39	38.61911467714036	40.0	38.0	41.0	35.0	41.0
40	38.515864347236835	40.0	38.0	41.0	35.0	41.0
41	38.41308769638176	40.0	38.0	41.0	35.0	41.0
42	38.27077277671773	40.0	37.0	41.0	35.0	41.0
43	37.45448055798278	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	7.0
11	4.0
12	3.0
13	4.0
14	4.0
15	8.0
16	8.0
17	13.0
18	24.0
19	50.0
20	105.0
21	201.0
22	349.0
23	644.0
24	1088.0
25	1595.0
26	2308.0
27	3602.0
28	5229.0
29	7186.0
30	10030.0
31	12697.0
32	16217.0
33	21394.0
34	31368.0
35	46556.0
36	77271.0
37	151879.0
38	339859.0
39	1064761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55709337161403	17.08504600816734	11.64119202168447	26.71666859853416
2	20.057855716247303	19.61950755211584	32.465739073060256	27.856897658576603
3	20.509745528469782	20.7992470174938	28.617180394814273	30.07382705922214
4	14.99066016370348	14.785863633931914	32.443169659575226	37.780306542789376
5	16.639341887048165	34.340950835458386	31.775728016833998	17.24397926065945
6	36.01225769923498	32.667470620753605	15.505131337268399	15.815140342743014
7	31.504226331651125	28.950037448508304	19.542270003744854	20.003466216095724
8	29.436422240847403	30.582391176418138	19.11211370080425	20.869072881930204
9	27.945782138844805	13.45003299035255	18.615029334664836	39.98915553613781
10	18.934679101949108	24.915963764110064	30.029245501720847	26.120111632219984
11	38.23297653226813	20.47569636392817	19.61989764074398	21.67142946305972
12	24.11695079979314	24.072703603973107	26.71388225119033	25.096463345043425
13	32.118281559284554	17.718717121101342	23.514653957950674	26.648347361663426
14	24.452204112202864	19.256167858480303	23.206038126147977	33.08558990316885
15	27.441453269611426	25.581454963710616	20.607824954972624	26.369266811705334
16	27.068695721953738	23.98471075485493	22.334301496157067	26.612292027034258
17	25.555151844784852	24.82886254614191	23.45251841218325	26.16346719688999
18	26.13421054977977	22.665375287551047	24.913957594022502	26.28645656864668
19	26.247670613620556	23.63429971289477	24.448693314549637	25.669336358935034
20	26.56542566471102	22.951588886709345	24.498011662535443	25.98497378604419
21	27.93051295540061	23.04509870356831	23.997137864008415	25.027250477022665
22	27.611364730638225	22.870561905950744	23.24298509192717	26.275088271483853
23	26.315546034916277	22.908289048986216	24.07398532375127	26.702179592346237
24	25.89647939440412	23.49397926065945	23.986271109367475	26.623270235568953
25	26.482448240811742	22.895081762576456	23.942469729122458	26.680000267489344
26	26.271410292989994	23.568876277261623	24.0198187313872	26.139894698361182
27	26.36475292900833	22.888227348110636	24.003936551527364	26.743083171353675
28	25.556767926244273	23.22805027016424	24.625905005617277	26.589276797974215
29	25.362726697219895	23.55277118961428	24.622282754070294	26.462219359095528
30	24.956978797011253	23.936952761381672	25.080915526864842	26.02515291474223
31	26.240816199154732	23.662831909695598	23.850074451201028	26.24627743994864
32	25.313575530074715	23.405707776806892	24.527324036592542	26.753392656525847
33	25.026916115341407	23.036293845961804	25.00406806712199	26.932721971574797
34	25.895532036307223	22.785021042495142	24.758980954758634	26.560465966439
35	25.44559266722542	22.920939065927207	25.64108279686859	25.992385469978778
36	24.864862153824205	22.976777466697577	25.489394047470444	26.668966332007777
37	25.18334165522407	22.245974285357633	26.028830893236087	26.54185316618221
38	24.361090554059597	21.869148670577957	26.92185521693386	26.847905558428593
39	24.653378390427445	21.55022335360308	27.107481676979866	26.688916578989602
40	24.09978690015514	22.223572052713234	27.205839738217097	26.470801308914528
41	23.493254810350052	21.863631702837168	28.324168108137027	26.31894537867575
42	23.68484405371186	21.796369277957094	28.322774934465112	26.196011733865937
43	22.3680720259643	21.37568655598552	28.555713572409363	27.700527845640817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	431.0
1	402.0
2	373.0
3	764.5
4	1156.0
5	1156.0
6	1391.0
7	1626.0
8	1523.0
9	1420.0
10	2196.0
11	2972.0
12	2972.0
13	4727.5
14	6483.0
15	9387.5
16	12292.0
17	11329.0
18	10366.0
19	10366.0
20	11388.5
21	12411.0
22	10565.0
23	8719.0
24	9612.0
25	10505.0
26	10505.0
27	12215.5
28	13926.0
29	16785.0
30	19644.0
31	23153.5
32	26663.0
33	26663.0
34	32534.0
35	38405.0
36	46142.5
37	53880.0
38	61612.5
39	69345.0
40	69345.0
41	75180.0
42	81015.0
43	87295.0
44	93575.0
45	102234.0
46	110893.0
47	110893.0
48	119737.0
49	128581.0
50	131467.5
51	134354.0
52	136461.0
53	138568.0
54	138568.0
55	136552.0
56	134536.0
57	130475.0
58	126414.0
59	123821.5
60	121229.0
61	121229.0
62	114721.0
63	108213.0
64	101900.5
65	95588.0
66	86006.5
67	76425.0
68	76425.0
69	65841.0
70	55257.0
71	47667.0
72	40077.0
73	31275.0
74	22473.0
75	22473.0
76	17766.0
77	13059.0
78	10533.0
79	8007.0
80	6997.0
81	5987.0
82	5987.0
83	4863.5
84	3740.0
85	3325.5
86	2911.0
87	2392.5
88	1874.0
89	1874.0
90	1363.0
91	852.0
92	500.0
93	148.0
94	96.0
95	44.0
96	44.0
97	31.0
98	18.0
99	13.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1794464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.30446767579138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.75726361232972	44.8548802257542
2	7.275467824248739	7.610789432793568
3	2.2973913447752965	3.604914939943271
4	1.1622652214597637	2.4316665482615485
5	0.6803335805836569	1.7792242887196645
6	0.4751528451152863	1.4911569977035677
7	0.32210247082086213	1.1793178791339622
8	0.25882892504018357	1.0830327314659287
9	0.2018780574028881	0.9503211895092826
>10	1.296357163624315	13.290855701113424
>50	0.14934868019334407	5.412378587410776
>100	0.11366615884394517	11.532382227850645
>500	0.008126374007626704	2.861508096283941
>1k	0.0016038896067684283	1.280226362396867
>5k	2.1385194756912376E-4	0.6373447916593724
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6326	0.3525286659414733	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5070	0.28253562066444354	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4644	0.2587959412950051	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2126	0.11847548905968579	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.5726946876616084E-5	0.0	0.0	0.0
3	0.0	5.5726946876616084E-5	0.0	0.0	0.0
4	0.0	5.5726946876616084E-5	0.0	0.0	0.0
5	0.0	5.5726946876616084E-5	0.0	0.0	0.0
6	0.0	5.5726946876616084E-5	0.0	5.5726946876616084E-5	0.0
7	0.0	5.5726946876616084E-5	0.0	1.1145389375323217E-4	0.0
8	0.0	5.5726946876616084E-5	0.0	1.6718084062984824E-4	0.0
9	0.0	5.5726946876616084E-5	0.0	3.343616812596965E-4	0.0
10	0.0	5.5726946876616084E-5	0.0	3.9008862813631257E-4	0.0
11	0.0	5.5726946876616084E-5	0.0	7.244503093960091E-4	0.0
12	0.0	5.5726946876616084E-5	0.0	0.0010030850437790894	0.0
13	0.0	5.5726946876616084E-5	0.0	0.0011702658844089378	0.0
14	0.0	5.5726946876616084E-5	0.0	0.0014489006187920182	0.0
15	0.0	5.5726946876616084E-5	0.0	0.0017832623000517147	0.0
16	0.0	5.5726946876616084E-5	0.0	0.0023962587156944916	0.0
17	0.0	5.5726946876616084E-5	0.0	0.0032321629188437327	0.0
18	0.0	5.5726946876616084E-5	0.0	0.003399343759473581	0.0
19	0.0	5.5726946876616084E-5	0.0	0.0037337054407332775	0.0
20	0.0	5.5726946876616084E-5	0.0	0.004235247962622822	0.0
21	0.0	5.5726946876616084E-5	0.0	0.005628421634538224	0.0
22	0.0	5.5726946876616084E-5	0.0	0.008637676765875492	0.0
23	0.0	2.2290778750646433E-4	0.0	0.012315655259732154	0.0
24	0.0	2.2290778750646433E-4	0.0	0.016829537956738055	0.0
25	0.0	2.2290778750646433E-4	0.0	0.0207861511849778	0.0
26	0.0	2.2290778750646433E-4	0.0	0.02830928901332097	0.0
27	0.0	2.7863473438308043E-4	0.0	0.07411683934589938	0.0
28	0.0	2.7863473438308043E-4	0.0	0.2300965636535478	0.0
29	0.0	2.7863473438308043E-4	0.0	0.43372282754070296	0.0
30	0.0	2.7863473438308043E-4	0.0	0.7143080050644649	0.0
31	0.0	2.7863473438308043E-4	0.0	1.2504569609643883	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2390	0.0	19.815899	1
ACCGTCC	95	1.6774175E-7	19.473684	8
TATACAC	800	0.0	17.34375	37
TATATAG	75	2.068081E-4	17.266666	5
CAAGACG	475	0.0	16.357895	4
AAGACGG	500	0.0	16.279999	5
AGTCGGT	485	0.0	16.020618	11
ACGGACC	475	0.0	15.968421	8
GTGTAAC	105	9.348862E-6	15.857144	1
ATACACA	610	0.0	15.770493	37
GCAGTCG	500	0.0	15.54	9
TCTAGAC	315	0.0	15.269842	3
TTATACA	1375	0.0	15.069091	37
TACCGTC	295	0.0	15.050847	7
TCGTTTA	320	0.0	15.031251	30
ATACCGT	360	0.0	14.902778	6
CGTTTAT	330	0.0	14.575757	31
CCCTAGT	90	8.2772056E-4	14.388888	1
AAGACTA	90	8.2772056E-4	14.388888	5
GTTCTAG	310	0.0	14.322581	1
>>END_MODULE