FastQCFastQC Report
Fri 10 Feb 2017
ERR1631394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156645
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16101.02780171725877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12140.7750007979826997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12000.766063391745667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.33579112004851736No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2490.1589581537872259No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC2270.14491365827188865No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2020.12895400427718726No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1810.1155478949216381No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG1670.10661048868460532No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC1620.10341855788566504No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1610.10278017172587697No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT1610.10278017172587697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGG200.001837086437.06
GCATCAG551.834269E-830.27272815
GCATCGT502.6954876E-729.59999827
ATCGTTT453.979265E-628.77777729
AAGAGGC556.2038816E-726.9090922
CGCATCG556.2038816E-726.9090913
TGCATCA556.2038816E-726.9090914
CTGCATC556.2038816E-726.9090913
AGCGTCA358.8350865E-426.428573
GTACTTA358.8350865E-426.4285714
CGATGCT358.8350865E-426.4285730
GTGCGTA358.8350865E-426.4285710
GCGTACT358.8350865E-426.4285712
GAATAAC509.025793E-625.8999981
TAACGCC509.025793E-625.8999984
AACGCCG509.025793E-625.8999985
CCGGTCG509.025793E-625.89999820
GAGGGGA509.025793E-625.8999989
CCGCATC509.025793E-625.89999812
AATAACG509.025793E-625.8999982
Produced by FastQC (version 0.11.5)