FastQCFastQC Report
Fri 10 Feb 2017
ERR1631393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594729
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21220.35680116490031594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17440.293242804706009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15200.25557859125753074No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8470.14241780710205826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.12947073372914386No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6540.10996605176475335No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6410.10778018223426132No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC6220.10458544984354218No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC6180.10391287460339078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT400.001930097723.1254
GGTATCA9400.022.436171
GCGACAA502.6999784E-422.19999916
GGGCCGT450.003823669220.5555576
TAAGGTG450.003823669220.5555575
CTAGCTT450.003823669220.5555574
TAGGTTA555.139082E-420.18181833
GTCCTAG555.139082E-420.1818181
TATAGGT555.139082E-420.1818185
TACCGAG555.139082E-420.18181826
ATACACA2700.019.18518637
CCTACCG701.21794204E-418.524
CGACAAA609.229672E-418.517
CCATATA609.229672E-418.51
CTTAACG500.007030988618.49999830
TCGGGTC500.007030988618.49999837
TAACGCT500.007030988618.49999832
CAGTCGG1156.389564E-817.69565210
TCCATTA2000.017.5749998
CCATTAT2000.017.5749999