##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631393.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594729
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7814264984556	34.0	31.0	34.0	31.0	34.0
2	32.94949128090273	34.0	33.0	34.0	31.0	34.0
3	33.06774682250235	34.0	33.0	34.0	31.0	34.0
4	36.4024858380876	37.0	37.0	37.0	35.0	37.0
5	36.38042200733443	37.0	37.0	37.0	35.0	37.0
6	36.42556357601529	37.0	37.0	37.0	35.0	37.0
7	36.420510854523656	37.0	37.0	37.0	35.0	37.0
8	36.418985790166616	37.0	37.0	37.0	35.0	37.0
9	38.259477846212306	39.0	39.0	39.0	37.0	39.0
10	38.22365648892185	39.0	39.0	39.0	37.0	39.0
11	38.27445609681048	39.0	39.0	39.0	37.0	39.0
12	38.06213250068519	39.0	39.0	39.0	37.0	39.0
13	38.18905249281605	39.0	39.0	39.0	37.0	39.0
14	39.68785278673143	41.0	40.0	41.0	37.0	41.0
15	39.682897588649624	41.0	40.0	41.0	37.0	41.0
16	39.65289400718647	41.0	40.0	41.0	37.0	41.0
17	39.66401335734427	41.0	40.0	41.0	37.0	41.0
18	39.636765652927636	41.0	40.0	41.0	37.0	41.0
19	39.64530063272516	41.0	40.0	41.0	37.0	41.0
20	39.613956945096	41.0	40.0	41.0	37.0	41.0
21	39.58244511365681	41.0	40.0	41.0	37.0	41.0
22	39.52403363548776	41.0	39.0	41.0	37.0	41.0
23	39.45022018431924	41.0	39.0	41.0	37.0	41.0
24	39.406864302900985	41.0	39.0	41.0	36.0	41.0
25	39.4051643689815	41.0	39.0	41.0	36.0	41.0
26	39.3203089138078	41.0	39.0	41.0	36.0	41.0
27	39.24900416828505	41.0	39.0	41.0	36.0	41.0
28	39.15124872000525	41.0	39.0	41.0	35.0	41.0
29	39.070469070786864	40.0	39.0	41.0	35.0	41.0
30	39.01613844288743	40.0	39.0	41.0	35.0	41.0
31	38.95352336946744	40.0	39.0	41.0	35.0	41.0
32	38.87109086659638	40.0	38.0	41.0	35.0	41.0
33	38.763557855762876	40.0	38.0	41.0	35.0	41.0
34	38.71627749781833	40.0	38.0	41.0	35.0	41.0
35	38.59910984666966	40.0	38.0	41.0	35.0	41.0
36	38.53736575818566	40.0	38.0	41.0	35.0	41.0
37	38.46255353278552	40.0	38.0	41.0	35.0	41.0
38	38.36775909700048	40.0	38.0	41.0	35.0	41.0
39	38.26708467217842	40.0	38.0	41.0	34.0	41.0
40	38.15578860287627	40.0	37.0	41.0	34.0	41.0
41	38.052719810199264	40.0	37.0	41.0	34.0	41.0
42	37.91653341269721	40.0	37.0	41.0	34.0	41.0
43	37.00210852337788	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	2.0
14	2.0
15	2.0
16	1.0
17	8.0
18	14.0
19	37.0
20	46.0
21	97.0
22	184.0
23	294.0
24	500.0
25	725.0
26	1055.0
27	1501.0
28	2129.0
29	3020.0
30	4127.0
31	5330.0
32	6922.0
33	9368.0
34	13444.0
35	19278.0
36	30876.0
37	58812.0
38	127219.0
39	309734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9373714750752	18.713397194352385	12.681406153054583	25.667825177517827
2	19.728649519360918	20.571890726700733	32.758785934434	26.940673819504347
3	20.370622586085428	22.013387610155213	29.070887748873858	28.5451020548855
4	14.823894580556859	15.744650084324121	33.60841660655525	35.823038728563766
5	16.21932006006097	34.674784649815294	32.17112331835172	16.934771971772015
6	34.08392730134229	34.158751296809136	15.943059780168783	15.81426162167979
7	29.750693172856877	29.93144776864757	20.553058619976493	19.764800438519057
8	28.59604962932697	30.696502104319784	19.905704951330776	20.801743315022474
9	27.300333429175303	14.282135224614908	19.996502608751214	38.42102873745857
10	19.429017249873475	24.901593835175348	29.94674885536101	25.72264005959017
11	37.06209046473268	21.439512786496035	20.366418990834482	21.13197775793681
12	23.525673037635627	25.46756589976275	26.49425200385386	24.512509058747767
13	30.807308875134726	18.15717747074718	24.662493337301527	26.373020316816564
14	23.807313919449026	20.60888236490906	23.657161497085227	31.92664221855669
15	26.765467969444906	26.55024389259646	21.186288208579032	25.4979999293796
16	26.234469817345378	24.946992663885567	23.268749295897795	25.549788222871257
17	25.230146839989303	25.329687975531712	23.86734125963254	25.572823924846443
18	25.285466153491758	23.07033960005313	25.894819321068923	25.74937492538619
19	25.652524090804384	24.526464994980905	25.09226891575827	24.728741998456442
20	26.195124165796525	23.232093945309547	24.644838237247555	25.92794365164638
21	27.769286515370865	23.89390798161852	24.201947441607857	24.134858061402756
22	27.259810770956182	23.390485414365198	24.092822108893294	25.25688170578532
23	25.55029265430137	23.423946032562732	24.79751281676192	26.22824849637398
24	24.77884885384772	24.686706045946977	24.982639151613593	25.55180594859171
25	25.404680114808592	23.529876632886577	24.64450194962748	26.420941302677353
26	25.152128112131745	25.0102147364598	24.932196008602237	24.90546114280622
27	26.287603261317336	23.46362797173166	24.427764578488688	25.82100418846231
28	24.642988655337135	24.27559443040444	25.56122200195383	25.520194912304596
29	24.385728625979226	24.40573773937373	25.848075341878403	25.36045829276864
30	24.27391299230406	24.751777700431628	25.82571221514337	25.14859709212095
31	25.070578364263383	24.480225447220498	24.662829624921603	25.78636656359451
32	24.27139083515349	23.689781396232572	25.116313480593682	26.92251428802026
33	23.76393281645926	23.399565180107242	26.184194818144064	26.652307185289438
34	25.411910298640223	22.921868615789712	25.868420742892983	25.797800342677085
35	24.944806794355078	22.723122632324973	26.812548236255505	25.519522337064444
36	23.33028993037165	23.497088589929195	26.731502919817263	26.441118559881893
37	23.925350874095596	21.942430922319243	27.73010900763205	26.402109195953116
38	23.106994950641386	21.383352753943395	28.626651802753862	26.883000492661363
39	23.246218025352725	21.134668058897415	29.251642344664546	26.367471571085314
40	22.547412350835423	21.737968049313217	29.627780047719217	26.086839552132147
41	21.246315548762546	21.965130336674353	30.89272593063395	25.895828183929147
42	21.958740871892914	22.27300165285365	30.365426942355256	25.40283053289818
43	20.591731696285198	21.615223067985585	30.52701314380163	27.266032091927585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	179.0
1	142.0
2	105.0
3	271.0
4	437.0
5	437.0
6	513.5
7	590.0
8	600.0
9	610.0
10	867.0
11	1124.0
12	1124.0
13	1844.5
14	2565.0
15	3579.5
16	4594.0
17	4296.0
18	3998.0
19	3998.0
20	4546.5
21	5095.0
22	4527.5
23	3960.0
24	4561.5
25	5163.0
26	5163.0
27	5702.0
28	6241.0
29	7302.0
30	8363.0
31	9825.5
32	11288.0
33	11288.0
34	13750.0
35	16212.0
36	19306.0
37	22400.0
38	24772.0
39	27144.0
40	27144.0
41	29095.5
42	31047.0
43	32542.0
44	34037.0
45	36014.0
46	37991.0
47	37991.0
48	40240.5
49	42490.0
50	42956.0
51	43422.0
52	44103.0
53	44784.0
54	44784.0
55	42782.0
56	40780.0
57	40111.5
58	39443.0
59	38253.5
60	37064.0
61	37064.0
62	34497.5
63	31931.0
64	29390.0
65	26849.0
66	23860.0
67	20871.0
68	20871.0
69	18295.5
70	15720.0
71	13445.0
72	11170.0
73	8460.0
74	5750.0
75	5750.0
76	4568.5
77	3387.0
78	2912.5
79	2438.0
80	2212.0
81	1986.0
82	1986.0
83	1606.0
84	1226.0
85	1133.5
86	1041.0
87	927.5
88	814.0
89	814.0
90	572.5
91	331.0
92	193.0
93	55.0
94	40.0
95	25.0
96	25.0
97	17.0
98	9.0
99	4.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	594729.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.6522373447907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6247291423926	50.774414273374056
2	5.151057328838771	5.8363784473999525
3	1.5451932323518416	2.6261596122828257
4	0.8049626438216532	1.8241173900589804
5	0.5147801420188411	1.4581723393018227
6	0.3357350314141552	1.141208441078129
7	0.25335169761905024	1.0047058353654057
8	0.1853337682411629	0.8399658101122235
9	0.15517618756155663	0.7911970387197358
>10	1.1630516384670464	13.564846007923705
>50	0.14763025640637162	5.822913230239503
>100	0.1142270439148939	12.076001684750196
>500	0.0038771581485473046	1.329894353882201
>1k	8.947288035109163E-4	0.9100255355113117
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2122	0.35680116490031594	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1744	0.293242804706009	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1520	0.25557859125753074	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	847	0.14241780710205826	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	770	0.12947073372914386	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	654	0.10996605176475335	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	641	0.10778018223426132	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	622	0.10458544984354218	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	618	0.10391287460339078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.3628762007569836E-4	0.0
8	0.0	0.0	0.0	3.3628762007569836E-4	0.0
9	0.0	0.0	0.0	3.3628762007569836E-4	0.0
10	0.0	0.0	0.0	5.044314301135475E-4	0.0
11	0.0	0.0	0.0	8.407190501892459E-4	0.0
12	0.0	0.0	0.0	0.0013451504803027934	0.0
13	0.0	0.0	0.0	0.0015132942903406425	0.0
14	0.0	0.0	0.0	0.001849581910416341	0.0
15	0.0	0.0	0.0	0.002690300960605587	0.0
16	0.0	0.0	0.0	0.003026588580681285	0.0
17	0.0	0.0	0.0	0.003867307630870531	0.0
18	0.0	0.0	0.0	0.004539882871021928	0.0
19	0.0	0.0	0.0	0.005716889541286872	0.0
20	0.0	0.0	0.0	0.00605317716136257	0.0
21	0.0	0.0	0.0	0.008575334311930308	0.0
22	0.0	0.0	0.0	0.01227449813276299	0.0
23	0.0	0.0	0.0	0.016814381003784917	0.0
24	0.0	0.0	0.0	0.02438085245548813	0.0
25	0.0	0.0	0.0	0.032619899147342736	0.0
26	0.0	0.0	0.0	0.04674397919052207	0.0
27	0.0	0.0	0.0	0.11719623559638087	0.0
28	0.0	0.0	0.0	0.3889166326175451	0.0
29	0.0	0.0	0.0	0.7361336003457036	0.0
30	0.0	0.0	0.0	1.2146708837134224	0.0
31	0.0	0.0	0.0	2.191754563843364	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGGT	40	0.0019300977	23.125	4
GGTATCA	940	0.0	22.43617	1
GCGACAA	50	2.6999784E-4	22.199999	16
GGGCCGT	45	0.0038236692	20.555557	6
TAAGGTG	45	0.0038236692	20.555557	5
CTAGCTT	45	0.0038236692	20.555557	4
TAGGTTA	55	5.139082E-4	20.181818	33
GTCCTAG	55	5.139082E-4	20.181818	1
TATAGGT	55	5.139082E-4	20.181818	5
TACCGAG	55	5.139082E-4	20.181818	26
ATACACA	270	0.0	19.185186	37
CCTACCG	70	1.21794204E-4	18.5	24
CGACAAA	60	9.229672E-4	18.5	17
CCATATA	60	9.229672E-4	18.5	1
CTTAACG	50	0.0070309886	18.499998	30
TCGGGTC	50	0.0070309886	18.499998	37
TAACGCT	50	0.0070309886	18.499998	32
CAGTCGG	115	6.389564E-8	17.695652	10
TCCATTA	200	0.0	17.574999	8
CCATTAT	200	0.0	17.574999	9
>>END_MODULE