FastQCFastQC Report
Fri 10 Feb 2017
ERR1631392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296615
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86162.9047755507981727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63682.146890750636347No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59432.0036073698228343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20870.7036056841360012No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA19630.6618006506751176No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT12840.4328843787401177No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT12390.41771319724221634No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT11830.39883350471149476No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT11500.3877079716130337No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA11350.3826509111137333No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC10570.356354196517371No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC10030.3381487787198894No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA8900.3000522562918261No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8770.2956694705257657No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA8050.2713955801291236No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA7650.2579100854643224No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT7090.2390303929336008No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.23869325556698076No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA6750.22756772246851978No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT6500.21913928830301904No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT6210.20936230467103822No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT6060.20430524417173776No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT5990.20194528260539757No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA5710.19250543634003675No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5670.19115688687355664No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.183739864807916No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5410.18239131534143585No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA5240.17665998010889536No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA5230.17632284274227533No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA5070.17092864487635487No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.1668829964769145No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC4850.1635116228107142No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG4690.15811742494479378No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4590.15474605127859348No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT4580.15440891391197345No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.15204895234563323No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC4280.14429479291337255No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT4240.14294624344689244No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.14294624344689244No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA4220.14227196871365236No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA4060.13687777084773192No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT4000.13485494664801173No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT3930.1324949850816715No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA3790.12777506194899113No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT3760.12676364984913102No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT3710.12507796301603089No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT3680.1240665509161708No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT3600.12136945198321054No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT3560.12002090251673045No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT3520.11867235305025033No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT3440.1159752541172901No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC3390.11428956728418994No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG3160.10653540785192928No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT3030.10215262208586888No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.10046693525276873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACT251.2314181E-437.029
CGTTTAC355.6125464E-737.028
ATGCCCC2550.029.74509837
TAGACCG250.005489851329.65
GACCGTT502.7092756E-729.67
TTACTGG250.005489851329.631
GCCGTTT502.7092756E-729.626
GTTATAC250.005489851329.63
CCGTTTA453.9950883E-628.77777727
ATTGTTA405.9255082E-527.7514
TGTTACG405.9255082E-527.7516
TACACTG405.9255082E-527.755
GTTGACT358.853823E-426.42857227
AAGTACT358.853823E-426.4285724
TATTGTT358.853823E-426.42857213
TTGTTCA358.853823E-426.4285724
CAGTATC358.853823E-426.4285721
TTATTGT358.853823E-426.42857212
TTCTGGA358.853823E-426.4285722
ATTGACT2950.025.71186631
Produced by FastQC (version 0.11.5)