##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631392.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	296615
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76555467525243	34.0	31.0	34.0	31.0	34.0
2	32.87609190364614	34.0	33.0	34.0	31.0	34.0
3	32.85940360399845	34.0	33.0	34.0	31.0	34.0
4	36.32207406907945	37.0	37.0	37.0	35.0	37.0
5	36.336806972000744	37.0	37.0	37.0	35.0	37.0
6	36.43260455472582	37.0	37.0	37.0	35.0	37.0
7	36.41691081030966	37.0	37.0	37.0	35.0	37.0
8	36.4424321089628	37.0	37.0	37.0	35.0	37.0
9	38.25817305261029	39.0	39.0	39.0	37.0	39.0
10	38.24183200445022	39.0	39.0	39.0	37.0	39.0
11	38.285359809854526	39.0	39.0	39.0	37.0	39.0
12	38.093636532205046	39.0	39.0	39.0	37.0	39.0
13	38.204814321595336	39.0	39.0	39.0	37.0	39.0
14	39.546341216728756	41.0	39.0	41.0	37.0	41.0
15	39.51167338131922	41.0	39.0	41.0	37.0	41.0
16	39.419712421826276	40.0	39.0	41.0	37.0	41.0
17	39.4559378318696	40.0	39.0	41.0	37.0	41.0
18	39.64182189032921	41.0	39.0	41.0	37.0	41.0
19	39.69316116851811	41.0	40.0	41.0	37.0	41.0
20	39.735812416769214	41.0	40.0	41.0	38.0	41.0
21	39.73665526018576	41.0	40.0	41.0	37.0	41.0
22	39.66455843433407	41.0	40.0	41.0	37.0	41.0
23	39.58905314970585	41.0	40.0	41.0	37.0	41.0
24	39.54054919677022	41.0	39.0	41.0	36.0	41.0
25	39.496185290696694	41.0	39.0	41.0	36.0	41.0
26	39.35431451544932	41.0	39.0	41.0	35.0	41.0
27	39.21702206564065	41.0	39.0	41.0	35.0	41.0
28	39.04231411088448	41.0	39.0	41.0	35.0	41.0
29	38.82431097550697	40.0	38.0	41.0	35.0	41.0
30	38.56916541644893	40.0	37.0	41.0	35.0	41.0
31	38.26835122970854	40.0	37.0	41.0	35.0	41.0
32	38.03196399372924	40.0	35.0	41.0	35.0	41.0
33	37.81707937899297	40.0	35.0	41.0	35.0	41.0
34	37.67412976417241	40.0	35.0	41.0	34.0	41.0
35	37.481189420629434	40.0	35.0	41.0	34.0	41.0
36	37.304842978271495	40.0	35.0	41.0	33.0	41.0
37	37.086667902837014	40.0	35.0	41.0	33.0	41.0
38	36.86061392714461	40.0	35.0	41.0	33.0	41.0
39	36.563022773629115	39.0	35.0	41.0	33.0	41.0
40	36.288801982367715	39.0	35.0	41.0	31.0	41.0
41	35.923503531513916	39.0	35.0	41.0	30.0	41.0
42	35.57347066062067	39.0	35.0	41.0	26.0	41.0
43	34.5696272946412	38.0	35.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	5.0
13	8.0
14	4.0
15	4.0
16	4.0
17	5.0
18	11.0
19	14.0
20	24.0
21	58.0
22	102.0
23	172.0
24	267.0
25	470.0
26	741.0
27	1187.0
28	1848.0
29	2617.0
30	3379.0
31	4078.0
32	4735.0
33	6270.0
34	8758.0
35	12070.0
36	19418.0
37	49205.0
38	59827.0
39	121331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.729649545707396	13.164202754412285	17.616438817996393	23.48970888188392
2	20.060684725991607	21.330344048682637	34.48274699526322	24.12622423006254
3	18.87935539335502	24.088127707634477	31.78834516123595	25.244171737774558
4	12.388112536452978	20.056301940225545	31.566171636633346	35.98941388668813
5	12.846956492422837	39.91032146047907	29.968477656221026	17.274244390877065
6	25.713466952109638	32.41407211368272	21.903140434570066	19.969320499637575
7	28.599362810377087	33.212413397838944	17.507543448578122	20.68068034320584
8	35.70588136136069	28.595654299344268	16.487702914552536	19.21076142474251
9	32.08165467019537	12.30113109586501	16.120560322303323	39.4966539116363
10	21.517118149790132	27.5643510948536	20.9038652799083	30.01466547544797
11	32.515550461035346	27.43185610977193	14.546465957554405	25.50612747163832
12	25.184161286516193	32.30517674426445	23.281695126679367	19.228966842539993
13	36.20990172445763	18.777539908635774	24.891863189656625	20.12069517724997
14	24.03654569054161	21.848187043811002	26.128145913052276	27.98712135259511
15	32.50004214217083	23.9337187937225	21.47902162736207	22.087217436744602
16	21.880552231006522	26.41066702627986	27.102472902584157	24.60630784012946
17	17.049710904708125	31.760362759806483	27.345548943917198	23.844377391568194
18	21.8616725384758	21.922020127100787	31.203748967516816	25.012558366906596
19	21.12839876607724	30.040962190044336	32.06041501609831	16.77022402778012
20	24.691603593884327	21.901454747736963	34.4790384842304	18.927903174148305
21	29.4209665728301	19.87020211385129	31.07125398243514	19.637577330883467
22	26.77106687119667	21.640510425973066	27.120678320381643	24.46774438244863
23	22.143519376970147	24.8257842657991	27.871820373211065	25.15887598401969
24	20.47165517590142	25.16258449505251	33.64529777657907	20.720462552467005
25	19.622069012018947	24.444481904151846	29.803617483943835	26.12983159988537
26	24.795441902803297	22.360972978440067	29.316791126544512	23.526793992212127
27	22.40480083610067	22.906124100264652	28.118942062943546	26.570133000691133
28	19.821991470424624	23.57871314667161	32.87628744331878	23.723007939584985
29	20.614264281981693	24.267821924042952	34.78583348785463	20.33208030612073
30	19.80614601419348	25.265748529238234	36.303625912377996	18.62447954419028
31	20.177671392208754	27.476021104799152	30.998432311245217	21.347875191746876
32	18.51592131213863	26.636549061915275	36.05515567317904	18.792373952767054
33	20.371862515381892	24.24354803364631	35.85455894004012	19.530030510931677
34	21.398445796739882	25.923840668880537	29.54132461271345	23.136388921666132
35	19.253577870303253	27.324646427186757	30.844360534699867	22.577415167810123
36	16.242941186386393	26.148711292416095	32.81290561839422	24.795441902803297
37	17.298855418640326	26.40426141631408	33.7535188712641	22.5433642937815
38	18.344992667262275	21.72917755339413	35.580129123611414	24.345700655732177
39	18.469059218178447	21.464861857964028	37.542605734706605	22.52347318915092
40	17.858503447229573	21.303710196719653	34.40587967567386	26.431906680376922
41	13.726547881934495	23.289112148745005	38.708089611112044	24.27625035820845
42	13.972321022200497	22.329956340711025	36.71425922492119	26.983463412167286
43	12.338553343559834	25.468705223943495	39.25526355713635	22.937477875360315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	38.5
2	67.0
3	319.5
4	572.0
5	572.0
6	884.5
7	1197.0
8	1200.5
9	1204.0
10	1920.0
11	2636.0
12	2636.0
13	4643.0
14	6650.0
15	11101.5
16	15553.0
17	13592.0
18	11631.0
19	11631.0
20	12299.5
21	12968.0
22	8872.0
23	4776.0
24	4035.5
25	3295.0
26	3295.0
27	2952.5
28	2610.0
29	2561.0
30	2512.0
31	2434.0
32	2356.0
33	2356.0
34	2399.5
35	2443.0
36	2493.5
37	2544.0
38	3297.5
39	4051.0
40	4051.0
41	5735.0
42	7419.0
43	10488.0
44	13557.0
45	18535.5
46	23514.0
47	23514.0
48	32159.0
49	40804.0
50	37595.5
51	34387.0
52	27092.0
53	19797.0
54	19797.0
55	15618.5
56	11440.0
57	11073.0
58	10706.0
59	10377.0
60	10048.0
61	10048.0
62	9972.0
63	9896.0
64	9547.0
65	9198.0
66	8705.0
67	8212.0
68	8212.0
69	7527.0
70	6842.0
71	6103.5
72	5365.0
73	4554.5
74	3744.0
75	3744.0
76	3009.0
77	2274.0
78	1775.0
79	1276.0
80	953.0
81	630.0
82	630.0
83	459.0
84	288.0
85	191.5
86	95.0
87	62.5
88	30.0
89	30.0
90	23.0
91	16.0
92	8.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	296615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.265308221409946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.25533043438205	43.47926056032214
2	6.122071766750757	6.032115050851355
3	1.7694353468771524	2.6151533322528104
4	0.8672917039005066	1.7090957244212106
5	0.5550294015162072	1.367184726882032
6	0.38622104830078774	1.141637939168062
7	0.27801208969292285	0.9587445901600076
8	0.19693396283058795	0.7761609902490084
9	0.17701072517832966	0.7848439140965129
>10	1.1374570791095848	11.086077856492357
>50	0.12280094898263849	4.234878621057927
>100	0.11182432784452556	10.703461376718554
>500	0.012348698780377058	3.9486102896903232
>1k	0.006174349390188529	4.089885570105505
>5k	0.002058116463396176	7.072889457532261
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8616	2.9047755507981727	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6368	2.146890750636347	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5943	2.0036073698228343	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2087	0.7036056841360012	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1963	0.6618006506751176	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	1284	0.4328843787401177	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	1239	0.41771319724221634	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	1183	0.39883350471149476	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	1150	0.3877079716130337	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	1135	0.3826509111137333	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	1057	0.356354196517371	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	1003	0.3381487787198894	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	890	0.3000522562918261	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	877	0.2956694705257657	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	805	0.2713955801291236	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	765	0.2579100854643224	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	709	0.2390303929336008	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	708	0.23869325556698076	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	675	0.22756772246851978	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	650	0.21913928830301904	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	621	0.20936230467103822	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	606	0.20430524417173776	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	599	0.20194528260539757	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	571	0.19250543634003675	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	567	0.19115688687355664	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	545	0.183739864807916	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	541	0.18239131534143585	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	524	0.17665998010889536	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	523	0.17632284274227533	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	507	0.17092864487635487	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	495	0.1668829964769145	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	485	0.1635116228107142	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	469	0.15811742494479378	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	459	0.15474605127859348	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	458	0.15440891391197345	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.15204895234563323	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	428	0.14429479291337255	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	424	0.14294624344689244	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	424	0.14294624344689244	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	422	0.14227196871365236	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	406	0.13687777084773192	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	400	0.13485494664801173	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	393	0.1324949850816715	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	379	0.12777506194899113	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	376	0.12676364984913102	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	371	0.12507796301603089	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	368	0.1240665509161708	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	360	0.12136945198321054	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	356	0.12002090251673045	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	352	0.11867235305025033	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	344	0.1159752541172901	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	339	0.11428956728418994	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	316	0.10653540785192928	No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT	303	0.10215262208586888	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	298	0.10046693525276873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.371373666200293E-4	0.0
11	0.0	0.0	0.0	6.742747332400586E-4	0.0
12	0.0	0.0	0.0	0.001011412099860088	0.0
13	0.0	0.0	0.0	0.0016856868331001466	0.0
14	0.0	0.0	0.0	0.0023599615663402055	0.0
15	0.0	0.0	0.0	0.003034236299580264	0.0
16	0.0	0.0	0.0	0.008091296798880704	0.0
17	0.0	0.0	0.0	0.013485494664801173	0.0
18	0.0	0.0	0.0	0.015845456231141378	0.0
19	0.0	0.0	0.0	0.01989110463058173	0.0
20	0.0	0.0	0.0	0.023262478296782022	0.0
21	0.0	0.0	0.0	0.028993813529322524	0.0
22	0.0	0.0	0.0	0.04113075872764358	0.0
23	0.0	0.0	0.0	0.06304468755794548	0.0
24	0.0	0.0	0.0	0.09979266051952868	0.0
25	0.0	0.0	0.0	0.10990678151812956	0.0
26	0.0	0.0	0.0	0.13957486978069214	0.0
27	0.0	0.0	0.0	0.21307081570385852	0.0
28	0.0	0.0	0.0	0.46491242856902043	0.0
29	0.0	0.0	0.0	0.9385904286701616	0.0
30	0.0	0.0	0.0	1.6381504644067226	0.0
31	0.0	0.0	0.0	3.1158235423023113	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACT	25	1.2314181E-4	37.0	29
CGTTTAC	35	5.6125464E-7	37.0	28
ATGCCCC	255	0.0	29.745098	37
TAGACCG	25	0.0054898513	29.6	5
GACCGTT	50	2.7092756E-7	29.6	7
TTACTGG	25	0.0054898513	29.6	31
GCCGTTT	50	2.7092756E-7	29.6	26
GTTATAC	25	0.0054898513	29.6	3
CCGTTTA	45	3.9950883E-6	28.777777	27
ATTGTTA	40	5.9255082E-5	27.75	14
TGTTACG	40	5.9255082E-5	27.75	16
TACACTG	40	5.9255082E-5	27.75	5
GTTGACT	35	8.853823E-4	26.428572	27
AAGTACT	35	8.853823E-4	26.428572	4
TATTGTT	35	8.853823E-4	26.428572	13
TTGTTCA	35	8.853823E-4	26.428572	4
CAGTATC	35	8.853823E-4	26.428572	1
TTATTGT	35	8.853823E-4	26.428572	12
TTCTGGA	35	8.853823E-4	26.428572	2
ATTGACT	295	0.0	25.711866	31
>>END_MODULE