FastQCFastQC Report
Fri 10 Feb 2017
ERR1631391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281504
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.4916448789360009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11000.3907582130271684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9190.32646072524724334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.14209389564624303No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3020.1072808912129135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA250.00548938229.616
CCGCTTA451.3197478E-424.66666625
CGCTTAT451.3197478E-424.66666626
ACTCTAA400.001927586323.12510
GTACTGG652.670913E-622.769231
GTCGGTG903.79805E-922.6111112
AGTCGGT903.79805E-922.6111111
CTCCGCT603.7134352E-521.58333223
TAGGGTG603.7134352E-521.5833325
GCAGTCG957.0867827E-921.4210539
GGCACCG450.003818743220.5555559
TATGGGT450.003818743220.55555514
TATTAGA450.003818743220.5555552
ATGTACC555.129846E-420.18181835
TCTAATT555.129846E-420.18181812
CTCTAAA555.129846E-420.1818181
AAGCGTG1153.0213414E-919.30434822
AGAAGCG1254.0745363E-1019.2420
CTAGAAC500.00702201418.53
ACCGCCT500.00702201418.512
Produced by FastQC (version 0.11.5)