##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631391.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281504
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41537242810049	34.0	31.0	34.0	31.0	34.0
2	32.61479410594521	34.0	31.0	34.0	31.0	34.0
3	32.71568432420143	34.0	31.0	34.0	31.0	34.0
4	36.209705723542115	37.0	37.0	37.0	35.0	37.0
5	36.07914629987496	37.0	35.0	37.0	35.0	37.0
6	36.10916718767762	37.0	35.0	37.0	35.0	37.0
7	36.11344776628396	37.0	35.0	37.0	35.0	37.0
8	36.10523473911561	37.0	35.0	37.0	35.0	37.0
9	37.86907468455155	39.0	38.0	39.0	35.0	39.0
10	37.76839405479141	39.0	37.0	39.0	35.0	39.0
11	37.845604325338186	39.0	38.0	39.0	35.0	39.0
12	37.62594492440605	39.0	37.0	39.0	35.0	39.0
13	37.769754603842216	39.0	37.0	39.0	35.0	39.0
14	39.12606925656474	40.0	38.0	41.0	36.0	41.0
15	39.09171805729226	40.0	38.0	41.0	36.0	41.0
16	39.05816613618279	40.0	38.0	41.0	36.0	41.0
17	39.05448590428555	40.0	38.0	41.0	36.0	41.0
18	39.02218796180516	40.0	38.0	41.0	36.0	41.0
19	39.02424477094464	40.0	39.0	41.0	36.0	41.0
20	38.9918438103899	40.0	39.0	41.0	36.0	41.0
21	38.96148900193248	40.0	38.0	41.0	36.0	41.0
22	38.88535509264522	40.0	38.0	41.0	35.0	41.0
23	38.81102577583267	40.0	38.0	41.0	35.0	41.0
24	38.76545981584631	40.0	38.0	41.0	35.0	41.0
25	38.76081689780607	40.0	38.0	41.0	35.0	41.0
26	38.63445634875526	40.0	38.0	41.0	35.0	41.0
27	38.563402296237356	40.0	38.0	41.0	35.0	41.0
28	38.45462231442537	40.0	38.0	41.0	34.0	41.0
29	38.357437194498125	40.0	38.0	41.0	34.0	41.0
30	38.26885230760487	40.0	38.0	41.0	34.0	41.0
31	38.193009690803684	40.0	38.0	41.0	34.0	41.0
32	38.09626506195294	40.0	37.0	41.0	34.0	41.0
33	37.95880697965215	40.0	37.0	41.0	33.0	41.0
34	37.905560844606114	40.0	37.0	41.0	33.0	41.0
35	37.76605305786063	40.0	37.0	41.0	33.0	41.0
36	37.70795086393088	40.0	37.0	41.0	33.0	41.0
37	37.61402679890872	40.0	36.0	41.0	33.0	41.0
38	37.511346197567356	40.0	36.0	41.0	33.0	41.0
39	37.41892477549165	40.0	36.0	41.0	33.0	41.0
40	37.28422686711379	40.0	36.0	41.0	33.0	41.0
41	37.14675457542344	39.0	35.0	41.0	32.0	41.0
42	36.99611017960668	39.0	35.0	41.0	32.0	41.0
43	35.994270063658064	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	1.0
15	0.0
16	2.0
17	6.0
18	9.0
19	20.0
20	50.0
21	87.0
22	132.0
23	222.0
24	308.0
25	520.0
26	766.0
27	1157.0
28	1558.0
29	2108.0
30	2859.0
31	3609.0
32	4940.0
33	6488.0
34	9564.0
35	13659.0
36	21951.0
37	40798.0
38	81160.0
39	89528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.068290326247585	17.683940547914062	12.205155166534047	26.042613959304305
2	20.15246675002842	19.967389450949188	32.483730248948504	27.396413550073888
3	20.603970103444354	21.632729907923157	29.062819711265202	28.700480277367284
4	15.110975332499715	15.432107536660226	32.59101114016142	36.86590599067864
5	16.458380697965218	34.93058713197681	31.72139649880641	16.88963567125156
6	35.16681823348869	33.20308059565761	15.65483971808571	15.975261452767988
7	30.787484369671482	29.66742923723997	19.774496987609414	19.770589405479143
8	29.749133227236555	30.3441514152552	19.0299249744231	20.876790383085144
9	28.334588496078204	13.430004547004662	18.811455609867	39.42395134705013
10	19.664374218483573	24.928242582698648	29.467787313856995	25.93959588496078
11	38.028589291804025	20.863646697737863	19.577696942139365	21.530067068318743
12	23.922928271001478	25.00248664317381	26.046876776173693	25.02770830965102
13	31.58818347163806	17.753211322041604	23.91937592361032	26.739229282710014
14	24.023459702171195	19.83062407638968	23.577284869841993	32.56863135159713
15	27.435844606115722	25.66961748323292	20.970217119472547	25.92432079117881
16	26.539587359327044	24.448675684892578	22.623479595316585	26.388257360463797
17	25.354524269637373	25.011012276912588	23.733943389791975	25.900520063658067
18	25.48525065363192	22.63839945435944	25.40212572467887	26.474224167329773
19	26.06215186995567	23.96555643969535	24.87389166761396	25.098400022735024
20	26.208863817210414	22.905180743435263	24.62806922814596	26.257886211208366
21	28.469932931681257	23.18901329998863	23.73358815505286	24.607465613277256
22	27.84365408662044	23.196117994770944	23.33323860406957	25.626989314539045
23	26.08275548482437	23.259705013072637	24.11049221325452	26.547047288848468
24	25.95167386609071	23.995040923041948	24.105874161646014	25.947411049221326
25	25.82805217687848	22.867170626349893	24.374431624417415	26.930345572354213
26	25.872456519267935	24.063245992952144	24.62877969762419	25.435517790155735
27	26.699798226668182	23.199315107422986	23.943887120609297	26.156999545299538
28	25.289516312379217	23.386879049676025	25.396086734113904	25.92751790383085
29	25.220245538251678	23.621333977492327	25.39466579515744	25.763754689098555
30	24.561285097192226	24.057207002387177	25.64226440832102	25.73924349209958
31	25.239428214163915	24.177986813686484	24.212089348641584	26.370495623508017
32	24.74209957940207	23.402154143458	25.110478003864955	26.74526827327498
33	24.652580425144937	23.29096567011481	25.607806638626805	26.448647266113447
34	25.67210412640673	22.996120836648856	25.52894452654314	25.802830510401275
35	25.296621007161534	23.081732408775718	26.167656587473004	25.453989996589748
36	24.16626406729567	23.58794191201546	25.729652154143455	26.51614186654541
37	24.11368932590656	22.408562578151642	26.591806865977034	26.88594122996476
38	23.568048766624987	21.521541434579973	27.982195634875527	26.928214163919517
39	23.685276230533137	21.419589064453792	28.252529271342503	26.642605433670568
40	23.293807548027736	21.544276457883367	29.027296237353646	26.13461975673525
41	22.026329998863247	21.454757303626238	30.075238717744686	26.44367397976583
42	22.087430373991133	21.975531431169717	29.921777310446746	26.015260884392404
43	21.03309366829601	20.719066158917812	30.442551437990222	27.80528873479595
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80.0
1	65.5
2	51.0
3	114.0
4	177.0
5	177.0
6	244.0
7	311.0
8	291.0
9	271.0
10	419.0
11	567.0
12	567.0
13	967.0
14	1367.0
15	2081.0
16	2795.0
17	2480.5
18	2166.0
19	2166.0
20	2392.0
21	2618.0
22	2127.0
23	1636.0
24	1731.0
25	1826.0
26	1826.0
27	2075.5
28	2325.0
29	2762.0
30	3199.0
31	3823.5
32	4448.0
33	4448.0
34	5557.5
35	6667.0
36	8113.5
37	9560.0
38	10665.5
39	11771.0
40	11771.0
41	12711.0
42	13651.0
43	14555.0
44	15459.0
45	16312.5
46	17166.0
47	17166.0
48	18323.0
49	19480.0
50	19991.5
51	20503.0
52	20907.0
53	21311.0
54	21311.0
55	20692.5
56	20074.0
57	19362.5
58	18651.0
59	18127.5
60	17604.0
61	17604.0
62	16648.5
63	15693.0
64	14479.5
65	13266.0
66	11790.0
67	10314.0
68	10314.0
69	8997.0
70	7680.0
71	6846.5
72	6013.0
73	5238.5
74	4464.0
75	4464.0
76	3940.5
77	3417.0
78	2796.0
79	2175.0
80	1642.0
81	1109.0
82	1109.0
83	893.0
84	677.0
85	558.5
86	440.0
87	387.5
88	335.0
89	335.0
90	239.0
91	143.0
92	87.5
93	32.0
94	20.0
95	8.0
96	8.0
97	5.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	281504.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.681085258043026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.97480984550751	57.29693536847973
2	4.927174958475802	6.275356972239841
3	1.5249961581329086	2.9134023109274945
4	0.804423513992993	2.049062495126494
5	0.47729413705738183	1.5197304317557603
6	0.35906260940459045	1.3719297985481476
7	0.26795692092817425	1.194465126897683
8	0.20544730494750377	1.0466485873917721
9	0.15696075037494342	0.899588783411386
>10	1.1389450980049347	14.683387164679914
>50	0.11253838260420214	4.940295680164076
>100	0.04871064321674421	4.595872641261182
>500	5.598924507671748E-4	0.32766539622335344
>1k	0.0011197849015343495	0.8856592428931557
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1384	0.4916448789360009	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1100	0.3907582130271684	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	919	0.32646072524724334	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	400	0.14209389564624303	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	302	0.1072808912129135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	7.104694782312152E-4	0.0
12	0.0	0.0	0.0	7.104694782312152E-4	0.0
13	0.0	0.0	0.0	0.0010657042173468228	0.0
14	0.0	0.0	0.0	0.0010657042173468228	0.0
15	0.0	0.0	0.0	0.0010657042173468228	0.0
16	0.0	0.0	0.0	0.0021314084346936457	0.0
17	0.0	0.0	0.0	0.0028418779129248607	0.0
18	0.0	0.0	3.552347391156076E-4	0.003907582130271684	0.0
19	3.552347391156076E-4	0.0	3.552347391156076E-4	0.004262816869387291	0.0
20	3.552347391156076E-4	0.0	3.552347391156076E-4	0.0056837558258497215	0.0
21	3.552347391156076E-4	0.0	3.552347391156076E-4	0.006038990564965329	0.0
22	3.552347391156076E-4	0.0	3.552347391156076E-4	0.008170398999658974	0.0
23	3.552347391156076E-4	0.0	3.552347391156076E-4	0.011367511651699443	0.0
24	3.552347391156076E-4	0.0	3.552347391156076E-4	0.014919859042855518	0.0
25	3.552347391156076E-4	0.0	3.552347391156076E-4	0.018472206434011595	0.0
26	3.552347391156076E-4	0.0	3.552347391156076E-4	0.024866431738092533	0.0
27	3.552347391156076E-4	0.0	3.552347391156076E-4	0.074244060475162	0.0
28	3.552347391156076E-4	0.0	3.552347391156076E-4	0.2756621575537115	0.0
29	3.552347391156076E-4	0.0	3.552347391156076E-4	0.5427986813686484	0.0
30	3.552347391156076E-4	0.0	3.552347391156076E-4	0.8902182562237126	0.0
31	3.552347391156076E-4	0.0	3.552347391156076E-4	1.691983062407639	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTGGA	25	0.005489382	29.6	16
CCGCTTA	45	1.3197478E-4	24.666666	25
CGCTTAT	45	1.3197478E-4	24.666666	26
ACTCTAA	40	0.0019275863	23.125	10
GTACTGG	65	2.670913E-6	22.76923	1
GTCGGTG	90	3.79805E-9	22.61111	12
AGTCGGT	90	3.79805E-9	22.61111	11
CTCCGCT	60	3.7134352E-5	21.583332	23
TAGGGTG	60	3.7134352E-5	21.583332	5
GCAGTCG	95	7.0867827E-9	21.421053	9
GGCACCG	45	0.0038187432	20.555555	9
TATGGGT	45	0.0038187432	20.555555	14
TATTAGA	45	0.0038187432	20.555555	2
ATGTACC	55	5.129846E-4	20.181818	35
TCTAATT	55	5.129846E-4	20.181818	12
CTCTAAA	55	5.129846E-4	20.181818	1
AAGCGTG	115	3.0213414E-9	19.304348	22
AGAAGCG	125	4.0745363E-10	19.24	20
CTAGAAC	50	0.007022014	18.5	3
ACCGCCT	50	0.007022014	18.5	12
>>END_MODULE