FastQCFastQC Report
Fri 10 Feb 2017
ERR1631390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747226
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA34550.1977420207803684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31970.18297575699995305No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC28820.16494717912851573No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC27080.154988536113817No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC25660.14686136767653413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22970.13146553451013207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22360.12797428609693307No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT21380.12236539520359702No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC20880.11950371617638475No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT20800.11904584753203078No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC18770.10742743068154893No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC17500.10015876595242973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.021.6063441
AGTCGGT14400.019.91319511
TACTATA1600.019.656252
CTTATAC34250.019.2832137
GCAGTCG14800.019.259
CAGTCGG15050.019.05315810
TATACAC9450.018.01058237
TCGGTGA15850.017.85804613
CAAGACG6350.017.7716544
CGCCTTA11200.017.67410925
CGGTGAT15850.017.50788914
GCCTTAT11550.017.29870226
GTCGGTG16550.017.1027212
ATTTCGG650.001580378917.07692329
ACGGACC6350.016.8976388
TAAACTA1107.8133235E-716.8181825
AAGACGG6500.016.7923075
GTGATTC16650.016.77777716
GGTGATT16800.016.73809415
GGGCAGT17300.016.6820817
Produced by FastQC (version 0.11.5)