##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631390.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1747226
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83704454947443	34.0	33.0	34.0	31.0	34.0
2	33.01600994948564	34.0	33.0	34.0	31.0	34.0
3	33.123282277163916	34.0	33.0	34.0	31.0	34.0
4	36.45892803792984	37.0	37.0	37.0	35.0	37.0
5	36.44089602604357	37.0	37.0	37.0	35.0	37.0
6	36.48474267209851	37.0	37.0	37.0	35.0	37.0
7	36.46938175141624	37.0	37.0	37.0	35.0	37.0
8	36.45337351893802	37.0	37.0	37.0	35.0	37.0
9	38.31401375666342	39.0	39.0	39.0	37.0	39.0
10	38.27880823659904	39.0	39.0	39.0	37.0	39.0
11	38.333199597533465	39.0	39.0	39.0	37.0	39.0
12	38.11069031710838	39.0	39.0	39.0	37.0	39.0
13	38.23331040174539	39.0	39.0	39.0	37.0	39.0
14	39.76037845132799	41.0	40.0	41.0	38.0	41.0
15	39.769255379670405	41.0	40.0	41.0	38.0	41.0
16	39.746910817490125	41.0	40.0	41.0	38.0	41.0
17	39.73554251138662	41.0	40.0	41.0	38.0	41.0
18	39.72288587738507	41.0	40.0	41.0	38.0	41.0
19	39.74086237269821	41.0	40.0	41.0	38.0	41.0
20	39.72633248360544	41.0	40.0	41.0	38.0	41.0
21	39.69608052993717	41.0	40.0	41.0	37.0	41.0
22	39.6432825518851	41.0	40.0	41.0	37.0	41.0
23	39.57719779810969	41.0	40.0	41.0	37.0	41.0
24	39.544734338889185	41.0	39.0	41.0	37.0	41.0
25	39.527699335975996	41.0	39.0	41.0	37.0	41.0
26	39.46989799831275	41.0	39.0	41.0	37.0	41.0
27	39.40751854654177	41.0	39.0	41.0	36.0	41.0
28	39.29794199491079	41.0	39.0	41.0	36.0	41.0
29	39.22677661619046	41.0	39.0	41.0	36.0	41.0
30	39.16293370176497	41.0	39.0	41.0	35.0	41.0
31	39.1165401613758	41.0	39.0	41.0	35.0	41.0
32	39.03660430877288	40.0	39.0	41.0	35.0	41.0
33	38.9421711902181	40.0	39.0	41.0	35.0	41.0
34	38.89377046815924	40.0	39.0	41.0	35.0	41.0
35	38.779762320386716	40.0	38.0	41.0	35.0	41.0
36	38.72580879634346	40.0	38.0	41.0	35.0	41.0
37	38.65975895505218	40.0	38.0	41.0	35.0	41.0
38	38.567665545270046	40.0	38.0	41.0	35.0	41.0
39	38.48010560740282	40.0	38.0	41.0	35.0	41.0
40	38.3876350283249	40.0	38.0	41.0	35.0	41.0
41	38.301206026009226	40.0	37.0	41.0	34.0	41.0
42	38.19254692867437	40.0	37.0	41.0	34.0	41.0
43	37.252310805814474	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	5.0
13	2.0
14	3.0
15	2.0
16	6.0
17	20.0
18	52.0
19	84.0
20	151.0
21	271.0
22	464.0
23	828.0
24	1195.0
25	1756.0
26	2529.0
27	3605.0
28	5065.0
29	7052.0
30	9726.0
31	12631.0
32	17259.0
33	23266.0
34	35155.0
35	51664.0
36	86262.0
37	163852.0
38	380496.0
39	943820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.828117255581134	17.08204891639662	12.760226782339549	27.329607045682696
2	19.830348220550746	19.18303642459533	33.396595517694905	27.59001983715902
3	19.90818589009092	20.396331098552793	28.65164552267423	31.04383748868206
4	14.677093861927421	15.343464440204071	32.728794099904654	37.25064759796386
5	16.558933990222215	34.40934372542533	32.16172378387226	16.86999850048019
6	35.53243827644506	33.27669116645471	15.753600278384136	15.43727027871609
7	31.785813626857657	28.82866898729758	19.357713312416365	20.027804073428396
8	29.271656900710042	31.34500058950588	18.775018228895405	20.608324280888677
9	27.8930716461408	14.119982188909736	17.93620287243894	40.050743292510525
10	19.1793734754405	24.256221004037258	29.14104986990807	27.423355650614173
11	38.54069250343115	20.86856537162336	19.04985388266887	21.540888242276615
12	24.604086706585182	24.092304029358537	25.790023729042495	25.51358553501379
13	31.67209050231624	17.00615718859495	24.18525136416239	27.136500944926418
14	24.49705991096744	19.236435355243113	22.133255800909556	34.13324893287989
15	28.008912413162353	25.185122016270363	19.77111146468745	27.034854105879834
16	27.765440761527127	24.006110257058904	21.706121589307852	26.522327392106114
17	26.331453400991055	24.574325244702173	22.576987750869094	26.517233603437678
18	26.777016825528012	21.970769665744445	24.386198465453237	26.86601504327431
19	26.83127425988395	23.778835708717704	23.19144747159211	26.198442559806228
20	27.559170937245668	21.73885919737916	23.329952736509185	27.372017128865984
21	29.605614843185712	22.356752932934835	22.3859420590124	25.651690164867052
22	28.855683237314462	22.07304607417701	21.809084800706948	27.262185887801575
23	26.70976736838852	22.23455923847287	22.986379552502083	28.06929384063653
24	26.26082716259946	23.23362862045322	23.52735135580629	26.978192861141032
25	26.633303304781407	22.095367170589267	23.025241153691624	28.246088370937706
26	27.09981421979755	23.47372349083633	23.10405179410105	26.322410495265064
27	27.95940536599158	21.822362991393216	22.585000452145287	27.63323119046992
28	26.113336225536937	22.693343620115545	23.832062938623853	27.36125721572367
29	25.449369457643144	22.988783362884938	24.529110716072218	27.032736463399697
30	24.91801289587037	24.204653547966892	24.863469293611704	26.01386426255104
31	26.842778209573346	23.415402472261746	22.499836884295448	27.24198243386946
32	25.380517460248413	22.510310629535045	24.014237425496187	28.09493448472035
33	25.408332980392924	22.080772607550482	24.65891647674657	27.85197793531003
34	26.834937209038785	21.85990822023024	24.33663418470192	26.968520386029056
35	26.48896021464882	21.891501156690662	25.083646877965414	26.5358917506951
36	24.454249192720347	22.765915800245644	25.286597154575308	27.493237852458698
37	25.833406783095032	21.008158074570776	25.689349860865168	27.469085281469024
38	24.350084076129818	20.281635003142124	27.32548622788351	28.04279469284454
39	24.971354592937605	19.77202720197616	27.656468024170888	27.60015018091535
40	23.957576180757385	20.47582854192875	28.041707254814202	27.524888022499667
41	22.3600724806064	20.658689831767614	30.027483565377345	26.95375412224864
42	23.4800191846962	20.586632753862407	29.29729754479386	26.63605051664753
43	21.356767813665776	20.142156767355797	30.011572629986045	28.489502788992382
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	30.0
2	38.0
3	163.5
4	289.0
5	289.0
6	386.0
7	483.0
8	498.5
9	514.0
10	798.5
11	1083.0
12	1083.0
13	1792.0
14	2501.0
15	4319.0
16	6137.0
17	5659.5
18	5182.0
19	5182.0
20	5757.5
21	6333.0
22	5553.5
23	4774.0
24	5516.0
25	6258.0
26	6258.0
27	7495.0
28	8732.0
29	11846.5
30	14961.0
31	18328.0
32	21695.0
33	21695.0
34	27048.0
35	32401.0
36	40298.0
37	48195.0
38	57307.5
39	66420.0
40	66420.0
41	72907.0
42	79394.0
43	85807.0
44	92220.0
45	101672.5
46	111125.0
47	111125.0
48	123043.5
49	134962.0
50	140368.5
51	145775.0
52	151604.0
53	157433.0
54	157433.0
55	148801.0
56	140169.0
57	135189.0
58	130209.0
59	125214.0
60	120219.0
61	120219.0
62	113241.0
63	106263.0
64	98563.0
65	90863.0
66	81106.0
67	71349.0
68	71349.0
69	60823.0
70	50297.0
71	43595.0
72	36893.0
73	28387.5
74	19882.0
75	19882.0
76	15843.5
77	11805.0
78	10001.0
79	8197.0
80	7066.5
81	5936.0
82	5936.0
83	4649.5
84	3363.0
85	2849.5
86	2336.0
87	1915.0
88	1494.0
89	1494.0
90	1164.5
91	835.0
92	480.0
93	125.0
94	83.5
95	42.0
96	42.0
97	30.5
98	19.0
99	11.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1747226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.91360561428431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.94470099927928	43.016924205449016
2	6.474064464142862	6.333396718410736
3	1.8061442661008356	2.650350849436717
4	0.8913278339746318	1.7439223257627767
5	0.5398939424445872	1.3204079687137822
6	0.3501563088948137	1.0276444557980657
7	0.25343404477655956	0.8677461040803454
8	0.19576101885172606	0.7660301816611043
9	0.1553490945701244	0.6838815909905299
>10	1.0596775518091905	10.404827443521446
>50	0.15611303372028434	5.4066131468883745
>100	0.1490624594803771	14.815473498418285
>500	0.017267161098214495	5.751317735676275
>1k	0.00704782085641408	5.211463775192616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3455	0.1977420207803684	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3197	0.18297575699995305	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2882	0.16494717912851573	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2708	0.154988536113817	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2566	0.14686136767653413	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2297	0.13146553451013207	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2236	0.12797428609693307	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2138	0.12236539520359702	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2088	0.11950371617638475	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2080	0.11904584753203078	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1877	0.10742743068154893	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1750	0.10015876595242973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.717007416327367E-4	0.0
2	0.0	0.0	0.0	1.717007416327367E-4	0.0
3	0.0	0.0	0.0	1.717007416327367E-4	0.0
4	0.0	0.0	0.0	1.717007416327367E-4	0.0
5	0.0	1.1446716108849113E-4	0.0	2.2893432217698225E-4	0.0
6	0.0	1.1446716108849113E-4	0.0	2.2893432217698225E-4	0.0
7	0.0	1.1446716108849113E-4	0.0	2.2893432217698225E-4	0.0
8	0.0	1.1446716108849113E-4	0.0	3.434014832654734E-4	0.0
9	0.0	1.1446716108849113E-4	0.0	3.434014832654734E-4	5.723358054424556E-5
10	0.0	1.1446716108849113E-4	0.0	4.0063506380971894E-4	5.723358054424556E-5
11	0.0	1.1446716108849113E-4	0.0	5.151022248982101E-4	5.723358054424556E-5
12	0.0	1.1446716108849113E-4	0.0	5.151022248982101E-4	5.723358054424556E-5
13	0.0	1.1446716108849113E-4	0.0	5.151022248982101E-4	5.723358054424556E-5
14	0.0	1.1446716108849113E-4	0.0	6.295693859867012E-4	5.723358054424556E-5
15	0.0	1.1446716108849113E-4	0.0	6.868029665309468E-4	5.723358054424556E-5
16	0.0	1.1446716108849113E-4	0.0	0.0014880730941503847	5.723358054424556E-5
17	0.0	1.1446716108849113E-4	0.0	0.0020604088995928404	5.723358054424556E-5
18	0.0	1.1446716108849113E-4	0.0	0.0022893432217698227	5.723358054424556E-5
19	0.0	1.1446716108849113E-4	0.0	0.002518277543946805	5.723358054424556E-5
20	0.0	1.1446716108849113E-4	0.0	0.0029761461883007693	5.723358054424556E-5
21	0.0	1.1446716108849113E-4	0.0	0.004006350638097189	5.723358054424556E-5
22	0.0	1.1446716108849113E-4	0.0	0.005265489410070592	5.723358054424556E-5
23	0.0	1.717007416327367E-4	0.0	0.007383131890207678	5.723358054424556E-5
24	0.0	1.717007416327367E-4	0.0	0.01161841685048185	5.723358054424556E-5
25	0.0	1.717007416327367E-4	0.0	0.01327819068626497	5.723358054424556E-5
26	0.0	1.717007416327367E-4	0.0	0.019459417385043493	5.723358054424556E-5
27	0.0	1.717007416327367E-4	0.0	0.04882024420424146	5.723358054424556E-5
28	0.0	1.717007416327367E-4	0.0	0.20455281686513366	5.723358054424556E-5
29	0.0	1.717007416327367E-4	0.0	0.45214528629954	5.723358054424556E-5
30	0.0	1.717007416327367E-4	0.0	0.7974354777229734	5.723358054424556E-5
31	0.0	1.717007416327367E-4	0.0	1.7939865821593772	5.723358054424556E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2680	0.0	21.606344	1
AGTCGGT	1440	0.0	19.913195	11
TACTATA	160	0.0	19.65625	2
CTTATAC	3425	0.0	19.28321	37
GCAGTCG	1480	0.0	19.25	9
CAGTCGG	1505	0.0	19.053158	10
TATACAC	945	0.0	18.010582	37
TCGGTGA	1585	0.0	17.858046	13
CAAGACG	635	0.0	17.771654	4
CGCCTTA	1120	0.0	17.674109	25
CGGTGAT	1585	0.0	17.507889	14
GCCTTAT	1155	0.0	17.298702	26
GTCGGTG	1655	0.0	17.10272	12
ATTTCGG	65	0.0015803789	17.076923	29
ACGGACC	635	0.0	16.897638	8
TAAACTA	110	7.8133235E-7	16.818182	5
AAGACGG	650	0.0	16.792307	5
GTGATTC	1665	0.0	16.777777	16
GGTGATT	1680	0.0	16.738094	15
GGGCAGT	1730	0.0	16.682081	7
>>END_MODULE