FastQCFastQC Report
Fri 10 Feb 2017
ERR1631389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720403
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.1905877682352794No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11690.1622702848266873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11050.1533863684632074No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10340.1435307737474719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9850.13672902528168263No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT9350.12978846562271396No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT8540.11854475897518472No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC8530.11840594778200536No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC8220.11410280079344479No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC7750.10757867471401424No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG7410.10285909414591554No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT7350.10202622698683932No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC7300.10133217102094244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGC352.3870802E-531.71428536
GGTATCA6000.024.6666661
GTTCTAG2100.023.7857131
CAGCGTA400.001930491323.1259
ACTATCC400.001930491323.1258
GTACTAT400.001930491323.1251
GGGCCGT400.001930491323.1256
TCGTTTA2150.022.37209330
TAATACT502.7007467E-422.1999994
ACACGCA502.7007467E-422.19999937
TCTAGAC2350.022.0425553
CTAGACA2300.020.9130444
GGATAGA806.952705E-720.81251
CCTATCG1600.020.812519
TATCGCT1600.020.812521
GTATAGC450.003824443820.5555531
AGGATAG450.003824443820.55555335
ATAATAC555.1405345E-420.1818183
GCTCCTA1650.020.18181825
CCGGTCG2300.020.10869620
Produced by FastQC (version 0.11.5)