##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631389.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720403
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.841964844677214	34.0	33.0	34.0	31.0	34.0
2	33.00933366462938	34.0	33.0	34.0	31.0	34.0
3	33.12896531524716	34.0	33.0	34.0	31.0	34.0
4	36.444658059447285	37.0	37.0	37.0	35.0	37.0
5	36.42865035264984	37.0	37.0	37.0	35.0	37.0
6	36.47032563717808	37.0	37.0	37.0	35.0	37.0
7	36.46108775227199	37.0	37.0	37.0	35.0	37.0
8	36.45076575194717	37.0	37.0	37.0	35.0	37.0
9	38.305441537583825	39.0	39.0	39.0	37.0	39.0
10	38.26520433701692	39.0	39.0	39.0	37.0	39.0
11	38.322590272389206	39.0	39.0	39.0	37.0	39.0
12	38.10762864674356	39.0	39.0	39.0	37.0	39.0
13	38.23756286411911	39.0	39.0	39.0	37.0	39.0
14	39.76471502756096	41.0	40.0	41.0	38.0	41.0
15	39.76268421980475	41.0	40.0	41.0	38.0	41.0
16	39.74615735914481	41.0	40.0	41.0	38.0	41.0
17	39.74218041846022	41.0	40.0	41.0	38.0	41.0
18	39.72353807521623	41.0	40.0	41.0	38.0	41.0
19	39.72374351578214	41.0	40.0	41.0	38.0	41.0
20	39.691722549739524	41.0	40.0	41.0	37.0	41.0
21	39.657661059157164	41.0	40.0	41.0	37.0	41.0
22	39.6056943127666	41.0	40.0	41.0	37.0	41.0
23	39.546170685019355	41.0	40.0	41.0	37.0	41.0
24	39.509084498537625	41.0	39.0	41.0	37.0	41.0
25	39.508055907596166	41.0	39.0	41.0	37.0	41.0
26	39.44602812592396	41.0	39.0	41.0	37.0	41.0
27	39.37784406783425	41.0	39.0	41.0	36.0	41.0
28	39.29429222254766	41.0	39.0	41.0	36.0	41.0
29	39.22852764355507	41.0	39.0	41.0	36.0	41.0
30	39.177070334243474	41.0	39.0	41.0	35.0	41.0
31	39.14063656036968	41.0	39.0	41.0	35.0	41.0
32	39.059094701160326	41.0	39.0	41.0	35.0	41.0
33	38.95940466655469	40.0	39.0	41.0	35.0	41.0
34	38.92149255347354	40.0	39.0	41.0	35.0	41.0
35	38.834426008775644	40.0	38.0	41.0	35.0	41.0
36	38.78243427637031	40.0	38.0	41.0	35.0	41.0
37	38.72153225347479	40.0	38.0	41.0	35.0	41.0
38	38.63161730309285	40.0	38.0	41.0	35.0	41.0
39	38.5618813358634	40.0	38.0	41.0	35.0	41.0
40	38.463125500587864	40.0	38.0	41.0	35.0	41.0
41	38.37551342790077	40.0	38.0	41.0	35.0	41.0
42	38.261262099130626	40.0	37.0	41.0	34.0	41.0
43	37.35426559856081	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	0.0
14	1.0
15	3.0
16	3.0
17	8.0
18	16.0
19	26.0
20	46.0
21	98.0
22	171.0
23	314.0
24	497.0
25	747.0
26	1078.0
27	1567.0
28	2132.0
29	3096.0
30	4285.0
31	5605.0
32	7264.0
33	9875.0
34	14654.0
35	21190.0
36	34371.0
37	64918.0
38	149353.0
39	399082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35406293421876	17.70550650122223	11.390291267526647	26.550139297032356
2	19.85902335220703	20.203136300098695	32.38270801204326	27.555132335651017
3	19.928290137603536	21.257268501102857	29.263065256530023	29.551376104763584
4	14.201911985374851	14.785751863887297	34.130479745364745	36.88185640537311
5	16.222031279714273	34.70307591722966	32.45100311908751	16.623889683968557
6	35.61381615567953	34.21834723064729	15.381113071433628	14.786723542239551
7	30.95045412081849	29.691575409874748	19.988256573057026	19.36971389624974
8	29.115231335793997	30.855784887070154	19.57015725920075	20.4588265179351
9	27.489474641277173	13.60460742112401	18.931903392961996	39.97401454463682
10	18.362916312119744	24.83720917319889	30.571915997018333	26.22795851766303
11	38.31452673017742	20.614433865489175	20.234785252143592	20.836254152189817
12	24.09970530383688	24.619275599907276	26.715879861688528	24.56513923456732
13	31.107171957918002	17.584046707190282	24.5740231509308	26.73475818396092
14	24.07124900923511	19.514077537156286	23.093046530900065	33.32162692270854
15	26.809022172311884	25.859831233351333	20.996164646732453	26.334981947604323
16	26.843447348220373	24.61788748797548	22.49629721142194	26.042367952382207
17	25.56152597920886	24.807642389051683	23.376221364985987	26.25461026675347
18	26.164799424766414	22.566258052784345	24.977130855923697	26.291811666525543
19	26.01293997942818	23.69382137498039	24.203119642755514	26.090119002835916
20	26.372460969762756	22.907733588005602	24.100815793382317	26.618989648849322
21	28.173813823651482	23.518086404415307	23.682855290719225	24.625244481213986
22	27.770706118658584	22.92175351851672	23.3384647204412	25.969075642383498
23	26.11663194073317	22.89510176942628	24.19937174053967	26.788894549300878
24	25.604141015514926	23.93576928469204	23.885658443954284	26.57443125583875
25	26.1063599124379	22.962008764538737	24.082770338268997	26.84886098475437
26	26.255720756298906	24.08374201662125	23.800011937762612	25.86052528931723
27	26.5309833523736	23.05084792817354	23.877329772363524	26.54083894708934
28	25.50155954375537	23.319725209361984	24.971717219389703	26.206998027492943
29	25.07415987995608	23.557786405664608	24.79681511598369	26.57123859839562
30	24.40800496388827	24.487543777580047	25.29917282409984	25.805278434431838
31	26.37384908169455	23.797513336285384	23.536548293108165	26.292089288911903
32	25.39592422574587	23.38232905748588	23.868445856000044	27.353300860768208
33	24.805143787574455	22.920781840164466	25.20492002393105	27.06915434833003
34	26.18853613880009	22.681610154316402	24.940762323310704	26.189091383572805
35	25.808748714261327	23.007122402322032	25.58318052534484	25.6009483580718
36	24.81319483677886	23.352068217372775	25.30875079642922	26.525986149419147
37	25.428406044949842	22.539467492500727	25.69617283659285	26.33595362595658
38	24.432852167467374	21.928559431318302	26.879121824867468	26.759466576346853
39	25.112888202853124	21.64621746439146	26.67076622390523	26.570128108850184
40	24.18382488690358	22.501849659149116	27.307354355825836	26.00697109812147
41	23.725747949411648	21.98686013245364	28.17423025723102	26.11316166090369
42	24.393985033377152	22.21270594375648	28.189360677287574	25.20394834557879
43	22.654541971646424	21.19605276491075	28.4139571878518	27.735448075591023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	115.0
2	105.0
3	211.5
4	318.0
5	318.0
6	370.0
7	422.0
8	386.0
9	350.0
10	502.0
11	654.0
12	654.0
13	1028.0
14	1402.0
15	2094.5
16	2787.0
17	2617.5
18	2448.0
19	2448.0
20	2877.5
21	3307.0
22	3122.5
23	2938.0
24	3518.0
25	4098.0
26	4098.0
27	4862.5
28	5627.0
29	6827.5
30	8028.0
31	9491.0
32	10954.0
33	10954.0
34	13961.5
35	16969.0
36	20572.0
37	24175.0
38	27971.5
39	31768.0
40	31768.0
41	34136.0
42	36504.0
43	38865.0
44	41226.0
45	44834.0
46	48442.0
47	48442.0
48	50737.5
49	53033.0
50	54574.5
51	56116.0
52	57104.5
53	58093.0
54	58093.0
55	56314.5
56	54536.0
57	53082.5
58	51629.0
59	50592.0
60	49555.0
61	49555.0
62	45759.0
63	41963.0
64	38401.5
65	34840.0
66	30968.5
67	27097.0
68	27097.0
69	22579.5
70	18062.0
71	15462.0
72	12862.0
73	9870.0
74	6878.0
75	6878.0
76	5387.5
77	3897.0
78	3377.5
79	2858.0
80	2568.0
81	2278.0
82	2278.0
83	1849.0
84	1420.0
85	1287.0
86	1154.0
87	1038.5
88	923.0
89	923.0
90	684.0
91	445.0
92	261.0
93	77.0
94	52.0
95	27.0
96	27.0
97	19.5
98	12.0
99	6.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	720403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.14920541244486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.9931836340396	47.64760975494591
2	6.141707338652769	6.6513714452765775
3	1.9449957590262072	3.159599246455326
4	0.941642921765913	2.0395686398350867
5	0.5478739710178604	1.483347009838899
6	0.3769245529367771	1.2246099025180495
7	0.2661472048090638	1.008816176420782
8	0.18905799967909662	0.8189872367591469
9	0.16550880189719985	0.8065953100349206
>10	1.1487588514309544	12.594794672768215
>50	0.14866985063663726	5.73570226135194
>100	0.1270263369871059	13.453571740153228
>500	0.007472137469823531	2.7223297602720478
>1k	0.0010306396510101421	0.6530968433698984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1373	0.1905877682352794	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1169	0.1622702848266873	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1105	0.1533863684632074	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1034	0.1435307737474719	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	985	0.13672902528168263	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	935	0.12978846562271396	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	854	0.11854475897518472	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	853	0.11840594778200536	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	822	0.11410280079344479	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	775	0.10757867471401424	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	741	0.10285909414591554	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	735	0.10202622698683932	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	730	0.10133217102094244	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.388111931793732E-4	0.0
10	0.0	0.0	0.0	2.776223863587464E-4	0.0
11	0.0	0.0	0.0	2.776223863587464E-4	0.0
12	0.0	0.0	0.0	4.1643357953811966E-4	0.0
13	0.0	0.0	0.0	6.94055965896866E-4	0.0
14	0.0	0.0	0.0	6.94055965896866E-4	0.0
15	0.0	0.0	0.0	0.001388111931793732	0.0
16	0.0	1.388111931793732E-4	0.0	0.0016657343181524786	1.388111931793732E-4
17	0.0	1.388111931793732E-4	0.0	0.0018045455113318517	1.388111931793732E-4
18	0.0	1.388111931793732E-4	0.0	0.0018045455113318517	1.388111931793732E-4
19	0.0	1.388111931793732E-4	0.0	0.0020821678976905983	1.388111931793732E-4
20	0.0	1.388111931793732E-4	0.0	0.002637412670408091	1.388111931793732E-4
21	0.0	1.388111931793732E-4	0.0	0.0030538462499462107	1.388111931793732E-4
22	0.0	1.388111931793732E-4	0.0	0.004580769374919316	1.388111931793732E-4
23	0.0	1.388111931793732E-4	0.0	0.005830070113533675	1.388111931793732E-4
24	0.0	1.388111931793732E-4	0.0	0.008883916363479885	1.388111931793732E-4
25	0.0	1.388111931793732E-4	0.0	0.011382517840708603	1.388111931793732E-4
26	0.0	1.388111931793732E-4	0.0	0.017212587954242277	2.776223863587464E-4
27	0.0	1.388111931793732E-4	0.0	0.05108251909000934	2.776223863587464E-4
28	0.0	2.776223863587464E-4	0.0	0.1776783272695977	2.776223863587464E-4
29	0.0	4.1643357953811966E-4	0.0	0.3621584030049847	2.776223863587464E-4
30	0.0	4.1643357953811966E-4	0.0	0.6024405783984798	2.776223863587464E-4
31	0.0	4.1643357953811966E-4	0.0	1.1264528326506136	2.776223863587464E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACACGC	35	2.3870802E-5	31.714285	36
GGTATCA	600	0.0	24.666666	1
GTTCTAG	210	0.0	23.785713	1
CAGCGTA	40	0.0019304913	23.125	9
ACTATCC	40	0.0019304913	23.125	8
GTACTAT	40	0.0019304913	23.125	1
GGGCCGT	40	0.0019304913	23.125	6
TCGTTTA	215	0.0	22.372093	30
TAATACT	50	2.7007467E-4	22.199999	4
ACACGCA	50	2.7007467E-4	22.199999	37
TCTAGAC	235	0.0	22.042555	3
CTAGACA	230	0.0	20.913044	4
GGATAGA	80	6.952705E-7	20.8125	1
CCTATCG	160	0.0	20.8125	19
TATCGCT	160	0.0	20.8125	21
GTATAGC	45	0.0038244438	20.555553	1
AGGATAG	45	0.0038244438	20.555553	35
ATAATAC	55	5.1405345E-4	20.181818	3
GCTCCTA	165	0.0	20.181818	25
CCGGTCG	230	0.0	20.108696	20
>>END_MODULE