FastQCFastQC Report
Fri 10 Feb 2017
ERR1631388.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631388.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137495
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9330.6785701298229027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8130.5912942288810502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6660.48438125022728096No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.20364376886432234No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2370.17236990436015853No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1890.1374595439834176No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1610.11709516709698534No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1570.11418597039892359No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1530.11127677370086186No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG1480.10764027782828466No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1480.10764027782828466No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1470.10691297865376923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1850.021.00000237
GCGCGCA450.00380895220.55555520
GGTATCA4500.020.5555551
TCTCTGT500.007004182318.534
AAAGTTG902.1243104E-618.528
CAGGGCT500.007004182318.54
ACCATCG752.050634E-417.26666621
AGTTGAT752.050634E-417.26666630
GGCGACT752.050634E-417.26666614
CATCGAA904.400323E-516.44444523
GCGACTA803.3555448E-416.187515
ACGGCGA803.3555448E-416.187512
CCATCGA803.3555448E-416.187522
AGGCCCG700.002576956815.8571435
CGAGCCG700.002576956815.85714310
AAGTTGA1059.223933E-615.85714229
CGGACAT956.9876725E-515.57894821
ACATCTA956.9876725E-515.57894824
GACACAG855.3216075E-415.2352947
GAAAGTT855.3216075E-415.23529427