##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631388.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	137495
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73457216626059	34.0	31.0	34.0	31.0	34.0
2	32.8973344485254	34.0	33.0	34.0	31.0	34.0
3	32.990312374995455	34.0	33.0	34.0	31.0	34.0
4	36.36305320193462	37.0	37.0	37.0	35.0	37.0
5	36.333023019018874	37.0	37.0	37.0	35.0	37.0
6	36.3841666969708	37.0	37.0	37.0	35.0	37.0
7	36.38270482563002	37.0	37.0	37.0	35.0	37.0
8	36.39110513109568	37.0	37.0	37.0	35.0	37.0
9	38.20794937997746	39.0	39.0	39.0	37.0	39.0
10	38.1735044910724	39.0	38.0	39.0	37.0	39.0
11	38.22376086403142	39.0	39.0	39.0	37.0	39.0
12	38.0167351540056	39.0	38.0	39.0	37.0	39.0
13	38.143583403032835	39.0	39.0	39.0	37.0	39.0
14	39.631732062984106	41.0	39.0	41.0	37.0	41.0
15	39.6024800901851	41.0	39.0	41.0	37.0	41.0
16	39.55828939234154	41.0	39.0	41.0	37.0	41.0
17	39.56815156914797	41.0	39.0	41.0	37.0	41.0
18	39.56871886250409	41.0	39.0	41.0	37.0	41.0
19	39.59106876613695	41.0	40.0	41.0	37.0	41.0
20	39.549932724826355	41.0	40.0	41.0	37.0	41.0
21	39.52748099930906	41.0	40.0	41.0	37.0	41.0
22	39.461653151023675	41.0	39.0	41.0	37.0	41.0
23	39.389606894796174	41.0	39.0	41.0	36.0	41.0
24	39.349249063602315	41.0	39.0	41.0	36.0	41.0
25	39.34687079530165	41.0	39.0	41.0	36.0	41.0
26	39.24435070366195	41.0	39.0	41.0	36.0	41.0
27	39.15726390050547	41.0	39.0	41.0	35.0	41.0
28	39.056656605694755	40.0	39.0	41.0	35.0	41.0
29	38.962100440016	40.0	39.0	41.0	35.0	41.0
30	38.87907196625332	40.0	38.0	41.0	35.0	41.0
31	38.7975199098149	40.0	38.0	41.0	35.0	41.0
32	38.68909414887814	40.0	38.0	41.0	35.0	41.0
33	38.57316266046038	40.0	38.0	41.0	35.0	41.0
34	38.51318957052984	40.0	38.0	41.0	35.0	41.0
35	38.37795556202044	40.0	38.0	41.0	35.0	41.0
36	38.28269391614241	40.0	38.0	41.0	35.0	41.0
37	38.20277100985491	40.0	38.0	41.0	34.0	41.0
38	38.06376231862977	40.0	37.0	41.0	34.0	41.0
39	37.943467035164915	40.0	37.0	41.0	34.0	41.0
40	37.813505945670755	40.0	37.0	41.0	33.0	41.0
41	37.67136259500345	40.0	37.0	41.0	33.0	41.0
42	37.49451252772828	40.0	37.0	41.0	33.0	41.0
43	36.558013018655224	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	1.0
15	0.0
16	0.0
17	0.0
18	2.0
19	8.0
20	19.0
21	24.0
22	49.0
23	77.0
24	108.0
25	211.0
26	303.0
27	441.0
28	611.0
29	878.0
30	1096.0
31	1388.0
32	1785.0
33	2379.0
34	3338.0
35	4746.0
36	7314.0
37	14865.0
38	30584.0
39	67265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.139786901341864	18.070475290010545	12.769191606967526	25.020546201680062
2	19.4487072257173	21.145496199861814	33.19029782901197	26.215498745408922
3	20.37455907487545	22.828466489690534	29.448343576130043	27.348630859303974
4	14.19978908323939	16.207134804901997	33.65431470235281	35.9387614095058
5	15.045638023200844	36.307502090985125	32.45936215862395	16.187497727190078
6	32.933561220408016	34.1816066038765	16.795519837084985	16.089312338630496
7	29.355249281792066	30.47383541219681	20.554929270155277	19.615986035855848
8	29.181424779082874	31.161860431288414	19.639259609440344	20.01745518018837
9	27.6700970944398	14.366340594203425	19.638532310265827	38.32503000109095
10	19.461071311684062	25.68893414305975	29.798174479071964	25.051820066184227
11	35.946034401250955	22.401541874249972	20.096003491036036	21.556420233463037
12	22.978290119640715	26.59223971780792	26.654060147641733	23.775410014909635
13	30.33128477399178	18.716316956980254	25.716571511691335	25.235826757336632
14	23.22775373649951	20.93894323429943	24.53761954980181	31.29568347939925
15	26.825702752827375	26.55223826320957	21.916433324848175	24.705625659114876
16	25.6962071348049	25.336194043419763	24.11069493436125	24.856903887414088
17	24.049601803701954	26.51150950943671	24.67216989708717	24.766718789774174
18	24.155787483181207	23.603767409723993	27.140623295392558	25.099821811702245
19	24.83144841630605	25.312920469835266	26.53769227971926	23.317938834139422
20	25.190734208516673	23.469217062438634	26.782792101530966	24.557256627513727
21	26.767518818866144	24.290337830466562	25.68893414305975	23.25320920760755
22	26.149314520528023	23.861958616676972	25.299829084693986	24.688897778101023
23	24.35652205534747	24.008145750754572	26.29259245790756	25.3427397359904
24	23.866322411724063	25.260554929270157	26.365322375359103	24.507800283646677
25	24.290337830466562	24.14705989308702	26.21113495036183	25.351467326084588
26	24.443070657114806	25.39219607985745	26.212589548710863	23.952143714316882
27	25.33837594094331	24.111422233535766	25.57693007018437	24.973271755336558
28	23.429215607840284	24.865631477508273	27.169715262373177	24.535437652278265
29	23.473580857485725	24.742717917015163	27.40972398996327	24.373977235535836
30	22.83210298556311	25.83512127713735	27.5100912760464	23.822684461253136
31	24.231426597330813	25.42928833775774	26.073675406378417	24.265609658533037
32	23.364485981308412	24.683806683879414	26.718062474999094	25.233644859813083
33	23.01901887341358	24.323793592494273	27.11007673006291	25.547110804029238
34	24.245245281646604	24.192879741081494	26.468598858140297	25.093276119131602
35	23.894687079530165	24.361613149569074	27.18717044256155	24.556529328339213
36	22.36008582130259	25.246008945779845	27.44827084621259	24.94563438670497
37	23.856867522455364	23.724499072693554	27.3733590312375	25.045274373613584
38	22.737554092876106	23.27357358449398	28.785046728971963	25.203825593657953
39	23.40012364085967	22.840103276482782	28.394487072257174	25.36528601040038
40	22.320084366704243	23.321575330012003	28.994508891232407	25.363831412051347
41	21.135314011418597	23.802320084366706	29.86944979817448	25.19291610604022
42	22.42554274700898	23.48885414015055	29.424342703371032	24.661260409469435
43	20.89239608713044	23.129568347939923	29.870904396523514	26.107131168406124
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	28.5
2	30.0
3	88.0
4	146.0
5	146.0
6	191.5
7	237.0
8	235.5
9	234.0
10	323.5
11	413.0
12	413.0
13	713.0
14	1013.0
15	1481.0
16	1949.0
17	1778.5
18	1608.0
19	1608.0
20	1753.5
21	1899.0
22	1483.0
23	1067.0
24	1140.5
25	1214.0
26	1214.0
27	1322.5
28	1431.0
29	1767.0
30	2103.0
31	2424.0
32	2745.0
33	2745.0
34	3176.0
35	3607.0
36	4213.5
37	4820.0
38	5575.0
39	6330.0
40	6330.0
41	6852.0
42	7374.0
43	7584.5
44	7795.0
45	8463.5
46	9132.0
47	9132.0
48	9952.5
49	10773.0
50	10938.0
51	11103.0
52	11013.5
53	10924.0
54	10924.0
55	10044.5
56	9165.0
57	8587.5
58	8010.0
59	7682.0
60	7354.0
61	7354.0
62	6871.5
63	6389.0
64	5957.5
65	5526.0
66	4983.0
67	4440.0
68	4440.0
69	3758.5
70	3077.0
71	2652.5
72	2228.0
73	1681.5
74	1135.0
75	1135.0
76	934.0
77	733.0
78	603.0
79	473.0
80	416.0
81	359.0
82	359.0
83	286.0
84	213.0
85	194.0
86	175.0
87	161.0
88	147.0
89	147.0
90	114.0
91	81.0
92	45.5
93	10.0
94	7.0
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	137495.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.67860649478163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.52779906845115	54.60271282592094
2	5.235540357290254	6.45841666969708
3	1.77230116148812	3.2793919778901053
4	0.9681032957962384	2.3884504891086946
5	0.649725841636696	2.0037092257900286
6	0.44926596309179884	1.6626059129422888
7	0.3667236601615471	1.5833303029201062
8	0.2688520724013914	1.326593694316157
9	0.20045987854489714	1.1127677370086184
>10	1.4150109073757444	16.499509073057204
>50	0.114380048346206	4.8998145387104985
>100	0.028300218147514884	2.428451943707044
>500	0.0035375272684393605	1.754245608931234
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	933	0.6785701298229027	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	813	0.5912942288810502	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	666	0.48438125022728096	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	280	0.20364376886432234	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	237	0.17236990436015853	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	189	0.1374595439834176	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	161	0.11709516709698534	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	157	0.11418597039892359	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	153	0.11127677370086186	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	148	0.10764027782828466	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	148	0.10764027782828466	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	147	0.10691297865376923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	7.27299174515437E-4	0.0
11	0.0	0.0	0.0	0.002181897523546311	0.0
12	0.0	0.0	0.0	0.002181897523546311	0.0
13	0.0	0.0	0.0	0.004363795047092622	0.0
14	0.0	0.0	0.0	0.005091094221608058	0.0
15	0.0	0.0	0.0	0.005091094221608058	0.0
16	0.0	0.0	0.0	0.0065456925706389325	0.0
17	0.0	0.0	0.0	0.00945488926870068	0.0
18	0.0	0.0	0.0	0.00945488926870068	0.0
19	0.0	0.0	0.0	0.00945488926870068	0.0
20	0.0	0.0	0.0	0.010182188443216117	0.0
21	0.0	0.0	0.0	0.013091385141277865	0.0
22	0.0	0.0	0.0	0.023273573584493983	0.0
23	0.0	0.0	0.0	0.029819266155132914	0.0
24	0.0	0.0	0.0	0.046547147168987967	0.0
25	0.0	0.0	0.0	0.05163824139059602	0.0
26	0.0	0.0	0.0	0.07054801992799738	0.0
27	0.0	0.0	0.0	0.12436815884213971	0.0
28	0.0	0.0	0.0	0.2894650714571439	0.0
29	0.0	0.0	0.0	0.5120186188588676	0.0
30	0.0	0.0	0.0	0.7927561002218263	0.0
31	0.0	0.0	0.0	1.4022328084657625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	185	0.0	21.000002	37
GCGCGCA	45	0.003808952	20.555555	20
GGTATCA	450	0.0	20.555555	1
TCTCTGT	50	0.0070041823	18.5	34
AAAGTTG	90	2.1243104E-6	18.5	28
CAGGGCT	50	0.0070041823	18.5	4
ACCATCG	75	2.050634E-4	17.266666	21
AGTTGAT	75	2.050634E-4	17.266666	30
GGCGACT	75	2.050634E-4	17.266666	14
CATCGAA	90	4.400323E-5	16.444445	23
GCGACTA	80	3.3555448E-4	16.1875	15
ACGGCGA	80	3.3555448E-4	16.1875	12
CCATCGA	80	3.3555448E-4	16.1875	22
AGGCCCG	70	0.0025769568	15.857143	5
CGAGCCG	70	0.0025769568	15.857143	10
AAGTTGA	105	9.223933E-6	15.857142	29
CGGACAT	95	6.9876725E-5	15.578948	21
ACATCTA	95	6.9876725E-5	15.578948	24
GACACAG	85	5.3216075E-4	15.235294	7
GAAAGTT	85	5.3216075E-4	15.235294	27
>>END_MODULE