FastQCFastQC Report
Fri 10 Feb 2017
ERR1631387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693042
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60650.3582309239818032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48920.2889473503905987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44470.2626633007332364No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA27760.1639652176378377No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC21800.12876231068101085No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC20770.12267858682773373No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC20690.12220606458670251No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT19410.1146457087302028No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.11417318648917155No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG18670.11027487800066389No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC17310.10224199990313293No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT17250.10188760822235951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.021.2849461
GTACTAA759.270021E-619.7333321
GGACCGT1052.2613676E-819.3809536
CGCATTG500.00703622518.49999812
GCAGTCG7450.018.375849
ATACACA5650.018.0088537
AGTCGGT7200.017.9861111
TAGGGGT1351.1532393E-917.8148164
GCTTAGA953.6084257E-617.5263161
TCGTTTA4350.017.01149630
TTAGAAC1201.0420263E-716.9583343
CAGTCGG7750.016.94838510
TAGCACT1107.8129597E-716.8181824
TCGGTGA7900.016.39240513
CTTATAC23650.016.34883737
CGCCTTA6500.016.22307625
CTCTATG11700.016.1282041
GCCTTAT6550.016.09923726
GTTCAAA6600.015.9772731
ATTAGAG4550.015.8571423
Produced by FastQC (version 0.11.5)