##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631387.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1693042
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84715441199923	34.0	33.0	34.0	31.0	34.0
2	33.02445479793177	34.0	33.0	34.0	31.0	34.0
3	33.136571922019655	34.0	33.0	34.0	31.0	34.0
4	36.46046878931533	37.0	37.0	37.0	35.0	37.0
5	36.43656507044716	37.0	37.0	37.0	35.0	37.0
6	36.478448851239364	37.0	37.0	37.0	35.0	37.0
7	36.47153407889468	37.0	37.0	37.0	35.0	37.0
8	36.4610978345487	37.0	37.0	37.0	35.0	37.0
9	38.314142236282386	39.0	39.0	39.0	37.0	39.0
10	38.28447374607364	39.0	39.0	39.0	37.0	39.0
11	38.33096934393831	39.0	39.0	39.0	37.0	39.0
12	38.1272443329817	39.0	39.0	39.0	37.0	39.0
13	38.249634681242405	39.0	39.0	39.0	37.0	39.0
14	39.77977037781697	41.0	40.0	41.0	38.0	41.0
15	39.77806043795724	41.0	40.0	41.0	38.0	41.0
16	39.74787512654736	41.0	40.0	41.0	38.0	41.0
17	39.746834986964295	41.0	40.0	41.0	38.0	41.0
18	39.72562759813401	41.0	40.0	41.0	38.0	41.0
19	39.74287170666764	41.0	40.0	41.0	38.0	41.0
20	39.70687082777628	41.0	40.0	41.0	38.0	41.0
21	39.678277325665874	41.0	40.0	41.0	37.0	41.0
22	39.619203776397754	41.0	40.0	41.0	37.0	41.0
23	39.54867983192384	41.0	40.0	41.0	37.0	41.0
24	39.517844211779746	41.0	39.0	41.0	37.0	41.0
25	39.5115472622652	41.0	39.0	41.0	37.0	41.0
26	39.43767195379677	41.0	39.0	41.0	36.0	41.0
27	39.36734115278889	41.0	39.0	41.0	36.0	41.0
28	39.26620367362416	41.0	39.0	41.0	36.0	41.0
29	39.19216416367698	41.0	39.0	41.0	35.0	41.0
30	39.13197368996162	41.0	39.0	41.0	35.0	41.0
31	39.07589829431284	41.0	39.0	41.0	35.0	41.0
32	38.98518524643807	40.0	39.0	41.0	35.0	41.0
33	38.882172444629255	40.0	38.0	41.0	35.0	41.0
34	38.84033000953313	40.0	38.0	41.0	35.0	41.0
35	38.724762291780124	40.0	38.0	41.0	35.0	41.0
36	38.66100014057537	40.0	38.0	41.0	35.0	41.0
37	38.587083486410855	40.0	38.0	41.0	35.0	41.0
38	38.48442448563001	40.0	38.0	41.0	35.0	41.0
39	38.388731053334766	40.0	38.0	41.0	35.0	41.0
40	38.28243008738117	40.0	38.0	41.0	34.0	41.0
41	38.17135251222356	40.0	37.0	41.0	34.0	41.0
42	38.04405147657294	40.0	37.0	41.0	34.0	41.0
43	37.12514692488432	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	6.0
13	3.0
14	0.0
15	5.0
16	7.0
17	39.0
18	57.0
19	76.0
20	171.0
21	301.0
22	462.0
23	777.0
24	1212.0
25	1833.0
26	2631.0
27	3736.0
28	5354.0
29	7789.0
30	10311.0
31	13508.0
32	17628.0
33	23766.0
34	35495.0
35	52075.0
36	83891.0
37	161794.0
38	348828.0
39	921285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49691265780766	17.748880417615158	12.2497256417738	26.504481282803383
2	19.673404440055236	19.941442681280204	32.88518536456863	27.49996751409593
3	20.381301822400154	21.43780248806586	28.74293726912859	29.4379584204054
4	14.844286201996171	15.490046909645477	32.94696764758346	36.71869924077489
5	16.33763367949525	34.70486851477991	32.06506394997879	16.89243385574605
6	34.704455057819	33.50336258639774	15.996827013151474	15.795355342631783
7	30.70987016270122	29.444632797059967	19.98975808042565	19.855738959813166
8	29.09738801518214	30.62150850362838	19.468802309688716	20.812301171500767
9	27.862864595207913	13.882880637338	19.069934472978225	39.18432029447586
10	19.322084153848518	24.61935380220928	29.853128274431466	26.205433769510737
11	37.581229526497275	21.14401178470469	19.780844184609712	21.493914504188318
12	23.63508997414122	24.842029908295245	26.48008732211014	25.04279279545339
13	31.073298831334366	17.8198768843301	24.53205531817876	26.574768966156775
14	24.179022138848296	19.95685871939385	23.133035093045535	32.73108404871232
15	27.33789238542222	25.863032340603482	20.65902676956626	26.140048504408043
16	26.83418367648292	24.575940821314532	22.705579660752655	25.88429584144989
17	25.617970493348658	25.05962640029013	23.47821259011885	25.84419051624236
18	25.798710250543106	22.73670706338059	25.330795101361925	26.13378758471438
19	25.909752977185445	24.170693934350123	24.614687645079094	25.30486544338534
20	26.382924936298096	22.782305459640103	24.553850406546324	26.28091919751548
21	27.89771311048397	23.438756982992743	23.798346408417512	24.865183498105775
22	27.542907972749642	22.95164561776967	23.596874737897817	25.90857167158287
23	25.920443792888776	22.972613792215434	24.368326361661435	26.738616053234352
24	25.3133708437239	24.082036948876638	24.460409133382395	26.144183074017064
25	25.92014846648813	22.9463888078382	24.27317219537377	26.860290530299896
26	25.745078976186058	24.444284311907204	24.425915009787115	25.384721702119617
27	26.898210440142655	22.838771867443334	23.89344150942505	26.369576182988965
28	25.123062511148568	23.64808433576958	25.042851860733524	26.186001292348326
29	24.731755030294583	23.852036748054687	25.427248703812428	25.988959517838307
30	24.273231260653898	24.796608707876118	25.550577008721582	25.379583022748402
31	25.80449864799574	24.131592718904788	23.949021938026345	26.11488669507313
32	24.780129494720153	23.549090926273536	24.746875742007582	26.92390383699873
33	24.33855746047647	23.218384422831804	25.505037677742195	26.938020438949533
34	25.813712831695845	23.04343306308999	25.125188861233212	26.017665243980954
35	25.522166608979575	22.97917003830974	26.08133761595991	25.417325736750772
36	23.83030072496725	23.72297911097303	25.894691330752572	26.552028833307144
37	24.942145558113737	22.24020431861702	26.324450308970484	26.493199814298755
38	23.890370114858346	21.654217674458167	27.535761073854044	26.919651136829447
39	24.34316455232652	21.488067041455558	27.610655849057498	26.558112557160424
40	23.46167431168276	21.941334001164766	28.301601496005414	26.29539019114706
41	22.193660877875445	22.096793818464043	29.59583991418996	26.11370538947055
42	23.214131722662522	21.905894833087423	29.22148416873297	25.658489275517088
43	21.652032259093396	21.38239925530495	29.561286725314552	27.404281760287102
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	262.0
1	223.5
2	185.0
3	555.0
4	925.0
5	925.0
6	1141.0
7	1357.0
8	1305.5
9	1254.0
10	2011.5
11	2769.0
12	2769.0
13	4589.5
14	6410.0
15	9470.5
16	12531.0
17	11519.0
18	10507.0
19	10507.0
20	11719.0
21	12931.0
22	11229.0
23	9527.0
24	10533.5
25	11540.0
26	11540.0
27	13044.5
28	14549.0
29	17797.0
30	21045.0
31	25159.5
32	29274.0
33	29274.0
34	34718.0
35	40162.0
36	47860.0
37	55558.0
38	63002.5
39	70447.0
40	70447.0
41	76399.0
42	82351.0
43	86611.5
44	90872.0
45	97917.0
46	104962.0
47	104962.0
48	113332.5
49	121703.0
50	125338.5
51	128974.0
52	134352.0
53	139730.0
54	139730.0
55	132885.5
56	126041.0
57	120998.0
58	115955.0
59	111065.0
60	106175.0
61	106175.0
62	99905.0
63	93635.0
64	87211.0
65	80787.0
66	72280.5
67	63774.0
68	63774.0
69	54901.0
70	46028.0
71	40254.0
72	34480.0
73	27089.5
74	19699.0
75	19699.0
76	15802.0
77	11905.0
78	10046.5
79	8188.0
80	7190.0
81	6192.0
82	6192.0
83	5012.0
84	3832.0
85	3414.5
86	2997.0
87	2624.0
88	2251.0
89	2251.0
90	1645.5
91	1040.0
92	591.5
93	143.0
94	106.0
95	69.0
96	69.0
97	43.0
98	17.0
99	13.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1693042.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.25864063239956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.49355503893949	43.97307140353132
2	6.722944028414253	6.757720558316171
3	1.9686004871700067	2.968175533003322
4	0.9376788799920586	1.8850586343244717
5	0.5275892417946042	1.3257959052437587
6	0.36512145678931873	1.1010304850371544
7	0.25257023966330716	0.8885685836774031
8	0.20694601727289197	0.8320660409939704
9	0.14640922974555035	0.6622495976743236
>10	1.0632187312140242	10.807952278186388
>50	0.15750930329058752	5.59987453342225
>100	0.1388626741889466	14.235255748142578
>500	0.013803581170412837	4.717554028096362
>1k	0.005073111028442325	3.8860036744051896
>5k	1.1797932624284475E-4	0.35962299594530533
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6065	0.3582309239818032	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4892	0.2889473503905987	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4447	0.2626633007332364	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2776	0.1639652176378377	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2180	0.12876231068101085	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2077	0.12267858682773373	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2069	0.12220606458670251	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1941	0.1146457087302028	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1933	0.11417318648917155	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1867	0.11027487800066389	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1731	0.10224199990313293	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1725	0.10188760822235951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.9065280128904066E-5	0.0	0.0	0.0
3	0.0	5.9065280128904066E-5	0.0	0.0	0.0
4	0.0	5.9065280128904066E-5	0.0	0.0	0.0
5	0.0	5.9065280128904066E-5	0.0	0.0	0.0
6	0.0	5.9065280128904066E-5	0.0	0.0	0.0
7	0.0	5.9065280128904066E-5	0.0	0.0	0.0
8	0.0	5.9065280128904066E-5	0.0	0.0	0.0
9	0.0	5.9065280128904066E-5	0.0	0.0	0.0
10	0.0	5.9065280128904066E-5	0.0	0.0	0.0
11	0.0	5.9065280128904066E-5	0.0	5.9065280128904066E-5	0.0
12	0.0	5.9065280128904066E-5	0.0	2.953264006445203E-4	0.0
13	0.0	5.9065280128904066E-5	0.0	4.134569609023285E-4	0.0
14	0.0	1.1813056025780813E-4	0.0	6.497180814179447E-4	0.0
15	0.0	1.1813056025780813E-4	0.0	0.0010631750423202731	0.0
16	0.0	1.1813056025780813E-4	0.0	0.0017128931237382179	0.0
17	0.0	1.1813056025780813E-4	0.0	0.0023626112051561626	0.0
18	0.0	1.1813056025780813E-4	0.0	0.0028351334461873953	0.0
19	0.0	1.1813056025780813E-4	0.0	0.0034257862474764357	0.0
20	0.0	1.1813056025780813E-4	0.0	0.0037801779282498602	0.0
21	0.0	1.1813056025780813E-4	0.0	0.004488961289796709	0.0
22	0.0	1.1813056025780813E-4	0.0	0.005906528012890407	0.0
23	0.0	1.1813056025780813E-4	0.0	0.008032878097530952	0.0
24	0.0	1.1813056025780813E-4	0.0	0.012580904667456566	0.0
25	0.0	1.1813056025780813E-4	0.0	0.016242952035448618	0.0
26	0.0	1.1813056025780813E-4	0.0	0.025693396856073268	5.9065280128904066E-5
27	0.0	1.1813056025780813E-4	0.0	0.06957889999184899	5.9065280128904066E-5
28	0.0	1.1813056025780813E-4	0.0	0.23295346482839766	5.9065280128904066E-5
29	0.0	1.1813056025780813E-4	0.0	0.44109951200265557	5.9065280128904066E-5
30	0.0	1.1813056025780813E-4	0.0	0.7282749039893871	5.9065280128904066E-5
31	0.0	1.1813056025780813E-4	0.0	1.3892744539119526	5.9065280128904066E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2790	0.0	21.284946	1
GTACTAA	75	9.270021E-6	19.733332	1
GGACCGT	105	2.2613676E-8	19.380953	6
CGCATTG	50	0.007036225	18.499998	12
GCAGTCG	745	0.0	18.37584	9
ATACACA	565	0.0	18.00885	37
AGTCGGT	720	0.0	17.98611	11
TAGGGGT	135	1.1532393E-9	17.814816	4
GCTTAGA	95	3.6084257E-6	17.526316	1
TCGTTTA	435	0.0	17.011496	30
TTAGAAC	120	1.0420263E-7	16.958334	3
CAGTCGG	775	0.0	16.948385	10
TAGCACT	110	7.8129597E-7	16.818182	4
TCGGTGA	790	0.0	16.392405	13
CTTATAC	2365	0.0	16.348837	37
CGCCTTA	650	0.0	16.223076	25
CTCTATG	1170	0.0	16.128204	1
GCCTTAT	655	0.0	16.099237	26
GTTCAAA	660	0.0	15.977273	1
ATTAGAG	455	0.0	15.857142	3
>>END_MODULE