FastQCFastQC Report
Fri 10 Feb 2017
ERR1631386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961387
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19110.19877531108700242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16230.16881859230465984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13800.14354261083205827No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12980.13501326728986351No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10030.10432843381489453No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9980.10380835189158996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTT250.005495901729.68
CCGCTTA652.6820871E-622.7692325
GGTATCA11200.021.968751
CGCTTAT705.1004918E-621.14285726
CTTAACC450.003825360220.5555553
ATCCCGT450.003825360220.5555556
CGTGTTT450.003825360220.55555510
CGTGCTT656.900627E-519.92307710
TAGGACG1403.6379788E-1219.8214284
CTTATAC11400.019.31140337
AGACTAT500.00703406518.56
TTAGCAA500.00703406518.54
TGGAACG801.616222E-518.521
AACTCAC801.616222E-518.514
CGTTTGG1103.849709E-818.528
AATCGTC500.00703406518.528
ATAGATA1552.0008883E-1117.90322732
GTGCTAT852.7223645E-517.4117641
GGATAGA752.0668234E-417.2666661
GCGTTTG1201.04064384E-716.95833427
Produced by FastQC (version 0.11.5)