##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631386.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	961387
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84850429639677	34.0	33.0	34.0	31.0	34.0
2	33.019183741823014	34.0	33.0	34.0	31.0	34.0
3	33.134236264896444	34.0	33.0	34.0	31.0	34.0
4	36.45980130790202	37.0	37.0	37.0	35.0	37.0
5	36.437217270464444	37.0	37.0	37.0	35.0	37.0
6	36.479079704635076	37.0	37.0	37.0	35.0	37.0
7	36.46621495818021	37.0	37.0	37.0	35.0	37.0
8	36.4545162353974	37.0	37.0	37.0	35.0	37.0
9	38.311662213031795	39.0	39.0	39.0	37.0	39.0
10	38.27487889892416	39.0	39.0	39.0	37.0	39.0
11	38.32868865503694	39.0	39.0	39.0	37.0	39.0
12	38.10606550743873	39.0	39.0	39.0	37.0	39.0
13	38.24079480999847	39.0	39.0	39.0	37.0	39.0
14	39.769950082537	41.0	40.0	41.0	38.0	41.0
15	39.775797883682635	41.0	40.0	41.0	38.0	41.0
16	39.75322632821122	41.0	40.0	41.0	38.0	41.0
17	39.74821689912595	41.0	40.0	41.0	38.0	41.0
18	39.730761909616	41.0	40.0	41.0	38.0	41.0
19	39.74655783779061	41.0	40.0	41.0	38.0	41.0
20	39.71076475966494	41.0	40.0	41.0	38.0	41.0
21	39.684856358573604	41.0	40.0	41.0	37.0	41.0
22	39.639943123840865	41.0	40.0	41.0	37.0	41.0
23	39.577724683192095	41.0	40.0	41.0	37.0	41.0
24	39.554572716294274	41.0	40.0	41.0	37.0	41.0
25	39.539488260190744	41.0	40.0	41.0	37.0	41.0
26	39.47135128725477	41.0	39.0	41.0	37.0	41.0
27	39.40757156067224	41.0	39.0	41.0	36.0	41.0
28	39.331639599869774	41.0	39.0	41.0	36.0	41.0
29	39.269679119854956	41.0	39.0	41.0	36.0	41.0
30	39.21056764861601	41.0	39.0	41.0	36.0	41.0
31	39.158484564488596	41.0	39.0	41.0	35.0	41.0
32	39.092901193796045	41.0	39.0	41.0	35.0	41.0
33	39.00507287907991	40.0	39.0	41.0	35.0	41.0
34	38.97445981691036	40.0	39.0	41.0	35.0	41.0
35	38.87284100991588	40.0	39.0	41.0	35.0	41.0
36	38.82522438934581	40.0	38.0	41.0	35.0	41.0
37	38.76335232325796	40.0	38.0	41.0	35.0	41.0
38	38.678700668929366	40.0	38.0	41.0	35.0	41.0
39	38.60595681031676	40.0	38.0	41.0	35.0	41.0
40	38.51246688378353	40.0	38.0	41.0	35.0	41.0
41	38.42774345814953	40.0	38.0	41.0	35.0	41.0
42	38.31293641374389	40.0	38.0	41.0	34.0	41.0
43	37.406832004177296	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	1.0
14	0.0
15	1.0
16	4.0
17	8.0
18	16.0
19	34.0
20	61.0
21	136.0
22	230.0
23	366.0
24	591.0
25	976.0
26	1339.0
27	2026.0
28	2874.0
29	3959.0
30	5355.0
31	7170.0
32	9408.0
33	13006.0
34	19061.0
35	27649.0
36	44486.0
37	85010.0
38	203634.0
39	533983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95720661918666	18.1088365039261	12.104178650220982	26.829778226666264
2	19.42537188457926	19.257177390582562	33.72419223476082	27.593258490077353
3	19.52918023647085	21.89867347904642	28.542303983723517	30.029842300759213
4	14.26470297601278	15.404930584665696	34.28203210569729	36.04833433362423
5	15.962874471986826	34.95990688453245	33.03497966999762	16.0422389734831
6	34.67198953179105	34.71910895404244	15.649265072234178	14.959636441932334
7	30.970982549171143	29.052296317715964	20.74086710138581	19.23585403172708
8	28.48218251338951	31.754641991206455	19.098864453128655	20.66431104227538
9	27.48435333533738	13.998629064050169	19.071924209501482	39.445093391110966
10	18.915067501432826	24.45862072193612	30.682857163660422	25.943454612970633
11	38.006234742096574	20.915822660385462	20.284651238263052	20.79329135925491
12	23.570736862470575	24.865844867883588	26.758110937634893	24.80530733201094
13	30.823799364875953	17.985057006179613	24.064086574917283	27.127057054027148
14	23.6510375114288	19.599391296116963	23.518416620986137	33.231154571468096
15	27.024912964290138	26.036133211703504	20.776128655785858	26.1628251682205
16	26.84371642221083	24.419926626842262	22.71769849186644	26.018658459080473
17	25.465395309069084	25.042984770961123	23.5414042419962	25.950215677973592
18	25.71212217348477	23.363952289764683	24.897673881589828	26.02625165516072
19	26.06307345533068	24.10423689939639	24.451027525855874	25.381662119417054
20	26.084084765032188	23.335451800367593	24.354500320890544	26.22596311370967
21	27.56184554190976	23.81642356303965	23.797492581031364	24.824238314019222
22	27.284641876788427	23.256919429948606	23.589355795324877	25.869082897938085
23	25.721379631719586	23.251510577946238	24.482024408484822	26.545085381849347
24	25.520732025708693	24.270143032930548	23.725513242846013	26.483611698514746
25	25.959369119823755	23.09226149303038	24.357516796045715	26.590852591100152
26	25.70255266609596	24.437713428619276	24.39350646513839	25.466227440146373
27	26.369713757311054	23.469112854656863	23.73549881577346	26.42567457225862
28	25.19661697110529	23.867495607908157	25.127966157229086	25.807921263757468
29	25.344424253708443	23.833794299278022	25.151577876547115	25.67020357046642
30	24.75090676283328	24.485873014717278	25.128798288306374	25.634421934143063
31	25.96436190628748	23.97723289372542	24.003861088198615	26.05454411178849
32	24.85773158988004	23.460999576653315	24.544954321204678	27.136314512261972
33	24.350651714658092	23.23694828409371	25.44573621236817	26.966663788880023
34	26.065673864947208	22.822130942065993	25.112571732299273	25.999623460687527
35	25.844326998388784	23.064593134710577	25.603737100668095	25.487342766232533
36	24.59363398922598	23.614735793182142	25.203794101646892	26.587836115944985
37	25.119124764532906	22.242655663120054	25.885413470329848	26.75280610201719
38	24.13700206056458	22.074773218277343	26.98205821380984	26.806166507348237
39	24.386433350981445	21.691576857186547	27.209229997909272	26.712759793922743
40	24.001676744120733	22.150601162695146	27.992473374405936	25.855248718778185
41	22.848551103769864	22.135414770534656	28.83354986077407	26.182484264921413
42	23.423553678175384	22.466498922910336	28.620108239449877	25.4898391594644
43	22.194808126176035	21.533055886963314	28.94963214605565	27.322503840805002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	37.0
2	45.0
3	159.0
4	273.0
5	273.0
6	362.0
7	451.0
8	400.5
9	350.0
10	575.5
11	801.0
12	801.0
13	1324.5
14	1848.0
15	2796.0
16	3744.0
17	3426.5
18	3109.0
19	3109.0
20	3499.0
21	3889.0
22	3237.5
23	2586.0
24	3119.5
25	3653.0
26	3653.0
27	4621.5
28	5590.0
29	7617.5
30	9645.0
31	12403.5
32	15162.0
33	15162.0
34	19885.5
35	24609.0
36	30286.0
37	35963.0
38	41339.5
39	46716.0
40	46716.0
41	51001.0
42	55286.0
43	58975.0
44	62664.0
45	65532.5
46	68401.0
47	68401.0
48	71891.5
49	75382.0
50	76557.0
51	77732.0
52	77487.0
53	77242.0
54	77242.0
55	74429.0
56	71616.0
57	67230.0
58	62844.0
59	61519.0
60	60194.0
61	60194.0
62	55685.5
63	51177.0
64	46479.0
65	41781.0
66	37216.0
67	32651.0
68	32651.0
69	27810.5
70	22970.0
71	20029.5
72	17089.0
73	13179.5
74	9270.0
75	9270.0
76	7347.5
77	5425.0
78	4538.0
79	3651.0
80	3228.0
81	2805.0
82	2805.0
83	2232.0
84	1659.0
85	1530.5
86	1402.0
87	1238.5
88	1075.0
89	1075.0
90	793.5
91	512.0
92	286.0
93	60.0
94	42.5
95	25.0
96	25.0
97	17.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	961387.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.70466438117727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.76659154943752	43.99470904012921
2	6.597626768790112	6.690609020475471
3	2.1007999157770767	3.195610639844465
4	1.0359226819644103	2.1010444765541787
5	0.6125907259138673	1.5530603580242195
6	0.4282858978921864	1.3029655627088683
7	0.29763596974243395	1.0564072367489439
8	0.22934485266331078	0.9303083025474979
9	0.19694482179751222	0.8987418981768259
>10	1.4000582939762143	14.450411400508145
>50	0.18193587065729128	6.444725013351235
>100	0.1431969454943214	13.972718551839497
>500	0.008035512042189491	2.6549283078156933
>1k	0.0010301938515627552	0.753760191275734
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1911	0.19877531108700242	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1623	0.16881859230465984	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1380	0.14354261083205827	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1298	0.13501326728986351	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1003	0.10432843381489453	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	998	0.10380835189158996	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.0401638466091178E-4	0.0	0.0	0.0
4	0.0	2.0803276932182356E-4	0.0	0.0	0.0
5	0.0	3.1204915398273537E-4	0.0	0.0	0.0
6	0.0	3.1204915398273537E-4	0.0	0.0	0.0
7	0.0	3.1204915398273537E-4	0.0	0.0	0.0
8	0.0	3.1204915398273537E-4	0.0	0.0	0.0
9	0.0	3.1204915398273537E-4	0.0	0.0	0.0
10	0.0	3.1204915398273537E-4	0.0	1.0401638466091178E-4	0.0
11	0.0	3.1204915398273537E-4	0.0	1.0401638466091178E-4	0.0
12	0.0	3.1204915398273537E-4	0.0	2.0803276932182356E-4	0.0
13	0.0	3.1204915398273537E-4	0.0	2.0803276932182356E-4	0.0
14	0.0	3.1204915398273537E-4	0.0	2.0803276932182356E-4	0.0
15	0.0	3.1204915398273537E-4	0.0	7.281146926263825E-4	1.0401638466091178E-4
16	0.0	3.1204915398273537E-4	0.0	0.001872294923896412	1.0401638466091178E-4
17	0.0	3.1204915398273537E-4	0.0	0.0027044260011837066	1.0401638466091178E-4
18	0.0	3.1204915398273537E-4	0.0	0.0028084423858446184	1.0401638466091178E-4
19	0.0	3.1204915398273537E-4	0.0	0.003224507924488265	1.0401638466091178E-4
20	0.0	3.1204915398273537E-4	0.0	0.003952622617114648	2.0803276932182356E-4
21	0.0	3.1204915398273537E-4	0.0	0.004368688155758295	2.0803276932182356E-4
22	0.0	3.1204915398273537E-4	0.0	0.0052008192330455895	2.0803276932182356E-4
23	0.0	4.160655386436471E-4	0.0	0.007177130541602913	2.0803276932182356E-4
24	0.0	4.160655386436471E-4	0.0	0.010817704004734826	2.0803276932182356E-4
25	0.0	4.160655386436471E-4	0.0	0.01341811362125762	2.0803276932182356E-4
26	0.0	4.160655386436471E-4	0.0	0.01861893285430321	2.0803276932182356E-4
27	0.0	4.160655386436471E-4	0.0	0.04472704540419207	2.0803276932182356E-4
28	0.0	4.160655386436471E-4	0.0	0.16049728153178688	2.0803276932182356E-4
29	0.0	4.160655386436471E-4	0.0	0.31745800598510276	2.0803276932182356E-4
30	0.0	4.160655386436471E-4	0.0	0.5426534787759768	2.0803276932182356E-4
31	0.0	4.160655386436471E-4	0.0	1.1617590002777238	2.0803276932182356E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTT	25	0.0054959017	29.6	8
CCGCTTA	65	2.6820871E-6	22.76923	25
GGTATCA	1120	0.0	21.96875	1
CGCTTAT	70	5.1004918E-6	21.142857	26
CTTAACC	45	0.0038253602	20.555555	3
ATCCCGT	45	0.0038253602	20.555555	6
CGTGTTT	45	0.0038253602	20.555555	10
CGTGCTT	65	6.900627E-5	19.923077	10
TAGGACG	140	3.6379788E-12	19.821428	4
CTTATAC	1140	0.0	19.311403	37
AGACTAT	50	0.007034065	18.5	6
TTAGCAA	50	0.007034065	18.5	4
TGGAACG	80	1.616222E-5	18.5	21
AACTCAC	80	1.616222E-5	18.5	14
CGTTTGG	110	3.849709E-8	18.5	28
AATCGTC	50	0.007034065	18.5	28
ATAGATA	155	2.0008883E-11	17.903227	32
GTGCTAT	85	2.7223645E-5	17.411764	1
GGATAGA	75	2.0668234E-4	17.266666	1
GCGTTTG	120	1.04064384E-7	16.958334	27
>>END_MODULE