FastQCFastQC Report
Fri 10 Feb 2017
ERR1631385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT105.025125628140704RNA PCR Primer, Index 29 (95% over 23bp)
CTTTCCCCTCGCCGCCAATGGCCGCGCCCTCTCCATGGACTGT21.0050251256281406No Hit
ATGCTGGTCGACAGCACGCTCGACGACATCATGTTGACCCTGT10.5025125628140703No Hit
ATCAATAAGCGGAGGAATCACCGACTGCCCATAAGAGGCTGTC10.5025125628140703No Hit
GAAGAACACCAGTGGCGAAGGCGACTCACTGGCCCGTTACTGA10.5025125628140703No Hit
GGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCA10.5025125628140703No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG10.5025125628140703No Hit
ATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTAC10.5025125628140703No Hit
GTTCCAGTAGTGACTGATTCACATTTTTTTCCAAATGTAATGC10.5025125628140703No Hit
CGCCAAAGGCGCGGGCGACCAGGAAGTGCCCCATTTCGTGCTG10.5025125628140703No Hit
GGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTG10.5025125628140703No Hit
GCCTTAGCGTTGTCGATTGCGCTCTGTAAGCTGTCTCTTATAC10.5025125628140703No Hit
CCGTTATAGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGA10.5025125628140703No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA10.5025125628140703No Hit
CTCCTGACGTCATGGTCTGCCTGCCTTGGCCTCCCAAAGTGCT10.5025125628140703No Hit
CAGCACCTGGGCTGGGCCGGTGAGGTCGAGCTGGGTCCTGTCT10.5025125628140703No Hit
GGTATCAACGCAGAGTACATGGGCATCTCTATGGGCATATCAA10.5025125628140703No Hit
ACATAGCACTTATCCAGGTTAAGATCTAAATAGGCTGTAAGTT10.5025125628140703No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5025125628140703No Hit
CTTCTCTGCTGAGTTTGCCTTGTTAATCTTCAATAGTTTTACC10.5025125628140703No Hit
GGTTCAAGCGATTCTCCCATGTACTCTGCGTTGATACCCTGTC10.5025125628140703No Hit
CCCCATGTCTGGTTTGTCTGCCATTTTCTTAAAACAATCCGTG10.5025125628140703No Hit
ATGCGCGCCTCGACCAGCCCCATGTACTCTGCGTTGATCTGTC10.5025125628140703No Hit
TGCATGTGCTGAAGTCTCGCTGTCGTTGGCAGGACTGCCCCGC10.5025125628140703No Hit
GCTTCAGCGAACGGCGCGACACGAAGAAGGAGGTCGACATGAT10.5025125628140703No Hit
TCGGCGAGCCGTCCACGAGGTTGACGCCGACGAAGCGGGCCTG10.5025125628140703No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC10.5025125628140703RNA PCR Primer, Index 29 (95% over 21bp)
GACCACAAGGGCGAGTCCTACGGGCTCATCATCGACACGGTTG10.5025125628140703No Hit
CCGGTGTTCACGATCAAGCCGCGCTGACCGTGCGCGGCGTCTC10.5025125628140703No Hit
GTTGATACCAGTGCTTCCCATGTACTCTGCGTTGATACTGTCT10.5025125628140703No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG10.5025125628140703No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT10.5025125628140703No Hit
GATCCATTCCCAGTCGGTGTGGGCGCCGAATCCGTAGTTGCTG10.5025125628140703No Hit
GGGCCTGGCGCGGTGGCTCACGTCTGTAATCCCAGCACTTTGG10.5025125628140703No Hit
CTTTTTAAGAGATTATCTTTTCTTCTGTTAAGTCATGTAAATT10.5025125628140703No Hit
CTACATAATACACTGTAACCTTCTAGCAGGGGTAGATGCTGTC10.5025125628140703No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG10.5025125628140703No Hit
TTGCTGCGCCTCGCCACGGGCGGCGATGACTATGAGATCGTGT10.5025125628140703No Hit
GTTAATGAGAGACCTAGATCCAAGTGCGTCTGAGGAATTCCAA10.5025125628140703No Hit
AGGGTGATGATCACGCCGGAGAGGCCGGCCTTGAGGATCAGAT10.5025125628140703No Hit
CTTCCAGATGCAGGAGAAGACGGTGGCAAGGAGCAGAAAGAAG10.5025125628140703No Hit
CTCCAAGACCTTGCCGGCCTTGCTCTCCCTGGCGCTCGGCGTG10.5025125628140703No Hit
CAACAGAGATTGTTGATTTGCCTCTTAAGCAAGAGATTCATTG10.5025125628140703No Hit
GTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGGAAT10.5025125628140703No Hit
CCTCAGATACACCTAAGAAGCTTAGGGGCAAGGTTCATTCTCC10.5025125628140703No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT10.5025125628140703No Hit
CGTCAGGGCCGCGGCAACCACCCATGTACTCTGCGTTGATACC10.5025125628140703No Hit
TTGATGAACTGCTCATTCCCCTCCAGGACTTCGATGTTGTGCT10.5025125628140703No Hit
CAGGAAAACTATGCAGGGATCTTTCACTTCCAGTTCTGGCAAT10.5025125628140703No Hit
GGCTTATGGGCCGAGGGGCAGGCTCCAATTTTCAAGCACACGA10.5025125628140703No Hit
GGTGGGAACCAAGAAGCGGGTGCTCACCCTCCGCAAGTCCTTG10.5025125628140703No Hit
TCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTCT10.5025125628140703No Hit
CCCCAAAGTTCACAATGTGGCCGAGGACTTTGATTGCACATTG10.5025125628140703No Hit
GCGTTCATCACTCTCGCAAGGTCGGTCACCCGCCGCCCTGTCT10.5025125628140703No Hit
ATATTGAACTGGGCACAGCATGCGGAAAATACTACAGAGTGTG10.5025125628140703No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCCAAAACGTATC10.5025125628140703No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT10.5025125628140703No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA10.5025125628140703No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCCTG10.5025125628140703No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC10.5025125628140703No Hit
GGCATATGCAGAGCCCTCACCAATCGGGGTCCACAGCTGTTCA10.5025125628140703No Hit
TCGTAGGAGAGGCTCGCGTTCACCCCCGGCAGGGCGGCGGGGA10.5025125628140703No Hit
CAGTTGAGGAGACCAGACACCATGCAGAGGTCGTGAAGCTGTC10.5025125628140703No Hit
GCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAA10.5025125628140703No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGGATGCCGTCTT10.5025125628140703No Hit
CATGTGTTCAGAAAGGCTGCTGATGACACCTGGGAGCCTGTCT10.5025125628140703No Hit
GTGATGTTCCCGATCCGCAACGTGAAGGGCGAATGCCTGTCTC10.5025125628140703No Hit
GTTCCACACCCGCCTCTCAGACCCAAAGGAGATCATCATGAGA10.5025125628140703No Hit
CTCGAACAGGCGGCCAAGGCCGGAGCCCACGCTGGCGTAGAGC10.5025125628140703No Hit
CTACAAAATTGGTGGTATTGGTACTGTTCCTGTTGGCCGAGTG10.5025125628140703No Hit
AGATCACCCAGCTTGCGGGCGACGATGGCCAGTTCACGCCGGA10.5025125628140703No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC10.5025125628140703No Hit
ATTGGACCGTTTCTGTGGCTTCCCGCCGCCTTTCGGGAGCGCC10.5025125628140703No Hit
CTATGTATATTGATCTGCTAAGACCAGGATTTTTCTGATATGG10.5025125628140703No Hit
ACTTAGACATGCATGGCTTAATCTTTGAGACAAGCATATGCTA10.5025125628140703No Hit
AGATTGAGCGGCCTGTAACAGTGTTTCTGCAACTGAAACGCAA10.5025125628140703No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGC10.5025125628140703No Hit
GCCACGTGCAGCCTATTCGCTGCACTAACTGCTGTCTCTTATA10.5025125628140703No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGC10.5025125628140703No Hit
TTCCGAGCGCGTGCGCCACATCGCGGTGCAGACCGTCGACGAG10.5025125628140703No Hit
CCCCCGACCTCTGGAAGGAGACTGTATTCACCAAGTCTCCCTA10.5025125628140703No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC10.5025125628140703RNA PCR Primer, Index 29 (95% over 24bp)
GTACATGGGACACCGCCCGTCACACCATGGGAGTGGGTTGCAA10.5025125628140703No Hit
CCAGGGACCCACTACTCCAGTGCCAGCGGCGGTTCTTTTTTTG10.5025125628140703No Hit
CTCTAGTGATCCCTGAAAAGTTCCAGCATATTTTGCGAGTACT10.5025125628140703No Hit
GGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA10.5025125628140703No Hit
AACCGCTTCGAAGCCCTTCCAATCCAATAAGGAAGAACAACAT10.5025125628140703No Hit
GTCTCTGCTAGTGTGGAGATAAATCATATTCCCATGTACTCTG10.5025125628140703No Hit
GTTCCGACCTGCACGAATGGTGTAACGATGGCCACACTGTCTC10.5025125628140703No Hit
ACTTCCTCCTGCACTTAAAAAGGGCCAGGTTCCAAATTAGACT10.5025125628140703No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT10.5025125628140703No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATT10.5025125628140703No Hit
GAGTTGCCGGACCCCTCCGCGATCGAATGGCAGGCCAGGACCG10.5025125628140703No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCT10.5025125628140703No Hit
TGCTCATAAGGAAAGGTTAAAAAAAAGTAAAAGGAACTCGGCA10.5025125628140703No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT10.5025125628140703No Hit
CATTAACACTATTCTCACCAGACCTCCTAGGCGACCCAGACAA10.5025125628140703No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10.5025125628140703No Hit
ATCTCGGGGTTGCCGCGCTGCACCGAGTCAAAGTCCAACTCGG10.5025125628140703No Hit
CTCTATGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGTC10.5025125628140703No Hit
GCCGTTGGGTGCGCTCGGCCTGACAGAGCCGACATCAAGGGCT10.5025125628140703No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGAGGG10.5025125628140703ABI Solid3 Adapter B (95% over 23bp)
GTTCTGTGTAGACTGCATCTGGGGCTCGTGTCGGGACCTGAAT10.5025125628140703No Hit
TTGCCCGAGAGGGATTCACTGAGTGAAGAAGACTGGATGAGAA10.5025125628140703No Hit
GGGATGGGCGGGGGCGGGCGCGCCACATCGTCCGGCCTGTCTC10.5025125628140703No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCCTGTCTCTTATA10.5025125628140703ABI Solid3 Adapter B (100% over 23bp)
AACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGG10.5025125628140703No Hit
AGTCATAAGGCCTTCCTTTGCGGAAAATTTTCAGGGTGGGATA10.5025125628140703No Hit
CCAACACTTTCACCAATTTTATATTCAAACAAAACCACACGGC10.5025125628140703No Hit
ACACATATCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC10.5025125628140703No Hit
TTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGCTGTC10.5025125628140703No Hit
CTATTCACCTTCGCAGGCGGCGGCGCGCGTCGCTTTCACCCTG10.5025125628140703No Hit
GCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCT10.5025125628140703No Hit
GTCTCACTCTGTCACCCAGGCTGAAGTGCAAGTGCAGTGGTGT10.5025125628140703No Hit
AACTACATCCCGCTGCAGGATCCGGGCGGCGGCCCCAACTACT10.5025125628140703No Hit
ACCGAGGAGGGGATGCGCAGGGCTTCGCGGTACTTGGCCTGTC10.5025125628140703No Hit
ATCTTGTCGTCGATCTCGCCGAAGACCATGACGAAGCGGCCCC10.5025125628140703No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT10.5025125628140703No Hit
CTACTTCCCATATCTAACAACGTAAAAATAAAATGACAGTTTG10.5025125628140703No Hit
GCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGA10.5025125628140703No Hit
CCCCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATAT10.5025125628140703ABI Solid3 Adapter B (100% over 23bp)
GGCCAGCAGACTATTTATTCATGCTCCTCTTTAACTGGATTTG10.5025125628140703No Hit
TTCCGGGAGGACCAGGATGCTGCCTTCTTCAAGGCCTGGGCAA10.5025125628140703No Hit
CTCCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTG10.5025125628140703No Hit
AGCCGAAGGAGCACGGCGCGCGGCTCGCGGTGGAAGGCCTGTC10.5025125628140703No Hit
ACCTAATATTAAAGAATTCAAACACCGCTTTGACCCTGTGGAA10.5025125628140703No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTG10.5025125628140703No Hit
TCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT10.5025125628140703No Hit
GTCTTTGGCAGCCTTATCATTGGTTATGCCAGAAACTGTCTCT10.5025125628140703No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.5025125628140703No Hit
AGTTCTAGATGCTGTCCGAGGCAGTCCTGCCATCAATGTGGCC10.5025125628140703No Hit
CAACGAAATATTCAATCGGTTATTAATCATCGCTTTACGGTTA10.5025125628140703No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG10.5025125628140703No Hit
AGGGAGATCATGCTGGCTGCAAAGAAGGGACTGGACCTGTCTC10.5025125628140703No Hit
CATCATACCAGTGACAGGCAGCTGGAAGGACCAGGGCAGTGGT10.5025125628140703No Hit
GCGTTGCTGCATCAGGCTTCCGCCCATTGTGCAATATTCCCCA10.5025125628140703No Hit
CCTTAGAATACTCATCTCGCGCACCAGTGTCGGTTTGCGGCTG10.5025125628140703No Hit
AGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACT10.5025125628140703No Hit
GATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGC10.5025125628140703No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGCTGT10.5025125628140703No Hit
GGTATCAACGCAGAGTACATGGGGTCTGAAGCTGTCTCTTATA10.5025125628140703No Hit
TCCTGGTTCTCCCCCAAATGGAGAAAAGCTCACCCCAAACCAG10.5025125628140703No Hit
GTGATGGTGGCGCCCACGGCGGCGTCGGCGACCTCCTTGATGG10.5025125628140703No Hit
GTGCAACACCAGCGAGTTCACGATGGGGTGCGACTCGTACATC10.5025125628140703No Hit
GAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACG10.5025125628140703No Hit
TGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGG10.5025125628140703No Hit
TTCTTGGCCTGGAGGCGCTCTTCACGGCGCTTGCGTGCTTCCT10.5025125628140703No Hit
GGTATCAACGCAGAGTACATGGGGGCACATCGCCTGAAGGCTA10.5025125628140703No Hit
GCGTCTGGTAAAGGCCCAAGACACCCGATCCTTAGAGCCAATC10.5025125628140703No Hit
TCCTAGTAGTCCCCCATCCCTTCCTCCCTTACCCAGCTATGAT10.5025125628140703No Hit
AGCAATATGTCAGCGGCAGACGGGTGAGTAACGCGTGGGAACG10.5025125628140703No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA10.5025125628140703No Hit
CGTGAAGATCGAGAAGCCGCAATGAGCCGGGCGGCGCGCCTGT10.5025125628140703No Hit
AGCCTGAGACTAGTTCGGACTCCCCTTCGGCAAGGTCGAAGGG10.5025125628140703No Hit
ACTGAGTCTTTCAGAGCCGCACCATTGGGCAGATATGCAGGGA10.5025125628140703No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC10.5025125628140703No Hit
GTACACCATAAGAGCAAAAAGATAAGATTTTGGTGTATCGCCA10.5025125628140703No Hit
GGTGCGGGTCGGCCGGCCGGTGCGCCACCCGTGCGCCGAAGGC10.5025125628140703No Hit
GCCATCCTTATAGCGAAGGCGACGAACCGGGACCGAGAAGCCC10.5025125628140703No Hit
GTATCAACGCAGAGTACATGGGCCTCTATGGGCATATCAATAA10.5025125628140703No Hit
GTTTTTGTTTTTGTTTTCAGATGGAGTCTTGCTCTTGTCGCCC10.5025125628140703No Hit
GTCTTGTGGCGGCGATTACCGGAGGCGGGGGCCAGCGCAAGCT10.5025125628140703No Hit
GGCTTAGTCACTGTTTTTGAATCGGTTCTGTTAGTTGTCACGG10.5025125628140703No Hit
GTGGAAGCTTCAGCTGTGGGTGCCCAGATCTTCACATCATGGT10.5025125628140703No Hit
CCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACG10.5025125628140703No Hit
GGTCCTGACTGGAGCACCTTCTAAATCTAAGATTCTGCTGAGG10.5025125628140703No Hit
AACTTACTATCCGCCATCCCATACATTGGGACAGACCTAGTTC10.5025125628140703No Hit
GGCAAGCTTCAGCCCGGTCTCGATTTCGGCCAGGGCCCCCAGC10.5025125628140703No Hit
CCTCTATGTACTGGCTGCCTGTGTCACTCCTGCTGAGGACTGT10.5025125628140703No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5025125628140703No Hit
ACATTGTCTGCTCCCACAATGAAACATTTCGGATAATCATCCA10.5025125628140703No Hit
CTCCAGGTGGCATCTCAAGCCTCCAGAGGTGAAGTCCCCAGTG10.5025125628140703No Hit
ATCCTGCTGTTCGCCGCCGCCACCGCCCACACCCCCTCTCCTT10.5025125628140703No Hit
CTCCTCAATTGACACTTGCCTCTTGTCAGCAAGATCTGTTTTA10.5025125628140703No Hit
GCCACGTAGCGCATGACGCCAAGCGCCTGCACGCGGCAGCCCC10.5025125628140703No Hit
CCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTG10.5025125628140703No Hit
AGTCTTTCCTAGAAAAGTCCTGCTAAAACATGTCTAGCAATTT10.5025125628140703No Hit
CTTATACACATTTCCGAGCCCACGAGACTGCAGCTAATCTCGT10.5025125628140703No Hit
CTCCTTTCCTTCTGAAGGTTTTACGATGCATTGTTATCATTAA10.5025125628140703No Hit
GCGTTCACATTGGGACCGACCATCGCCTCCGGCATCCTCGCGC10.5025125628140703No Hit
GCCCAATGACGTGCTGCTGGCAGGCGGCAAGGTCAGCGGCTGT10.5025125628140703No Hit
GCTCATGGCTATGCAGCGGCCAGGTCCCTATGCTGTCTCTTAT10.5025125628140703No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATAGGCTGT10.5025125628140703No Hit
CTACAGGCGTGCGCCACCATGCCCACCCCATGTACTCTGCGTT10.5025125628140703No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTT10.5025125628140703No Hit
CATGAAGGCCGGTCCCTTGCCCGCGGCGATGGCGTGGTGCAGC10.5025125628140703No Hit
CATGGGCCTCTATGGGCATATCAATAAGGCGAGGAATCACCGA10.5025125628140703No Hit
TGTAAGTTCTTATTTGGAAGGCCAAGCTGCCAAGGAATTCATT10.5025125628140703No Hit
GACTCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGA10.5025125628140703No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers