FastQCFastQC Report
Fri 10 Feb 2017
ERR1631384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882416
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15500.17565411325270622No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12560.14233649435187032No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC11940.13531032982176208No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11540.13077732044749868No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT10610.12023807365233632No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10460.11853819513698753No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10050.11389186052836757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.100406157639934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA200.00184170437.01
CCGCTTA1200.027.74999825
CGCTTAT1400.023.78571526
TTGTCGG400.001930833323.12500222
AGGGTTA400.001930833323.12500223
AGCTATA400.001930833323.12500237
GGTATCA8350.021.0479031
GCAGTCG4650.020.6881719
AGTCGGT4650.020.68817111
GTGATTC4750.020.25263216
CAGTCGG4750.020.25263210
TCGGTGA5000.019.98000113
TATTAGA1303.274181E-1119.9230772
TCTAGAT2150.019.7906972
CTTATTG4850.019.45360828
GATATGC3850.019.2207834
TTATTGA4950.019.06060629
GTTCAAA3500.019.028571
CTAGTAA609.2345005E-418.530
GGGCAGT5300.018.57
Produced by FastQC (version 0.11.5)