##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631384.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	882416
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83722983264129	34.0	33.0	34.0	31.0	34.0
2	33.01297687258617	34.0	33.0	34.0	31.0	34.0
3	33.13193777084731	34.0	33.0	34.0	31.0	34.0
4	36.4619725843593	37.0	37.0	37.0	35.0	37.0
5	36.435391017388625	37.0	37.0	37.0	35.0	37.0
6	36.48144865913583	37.0	37.0	37.0	35.0	37.0
7	36.460757737847004	37.0	37.0	37.0	35.0	37.0
8	36.4504020779315	37.0	37.0	37.0	35.0	37.0
9	38.30343511450382	39.0	39.0	39.0	37.0	39.0
10	38.258703377998586	39.0	39.0	39.0	37.0	39.0
11	38.31559604540262	39.0	39.0	39.0	37.0	39.0
12	38.083903737012925	39.0	39.0	39.0	37.0	39.0
13	38.22502991786187	39.0	39.0	39.0	37.0	39.0
14	39.750761545574875	41.0	40.0	41.0	38.0	41.0
15	39.75166248118801	41.0	40.0	41.0	38.0	41.0
16	39.735178192598504	41.0	40.0	41.0	38.0	41.0
17	39.71120650577505	41.0	40.0	41.0	38.0	41.0
18	39.69831236060996	41.0	40.0	41.0	38.0	41.0
19	39.70720045874055	41.0	40.0	41.0	37.0	41.0
20	39.67938591322007	41.0	40.0	41.0	37.0	41.0
21	39.6446131983101	41.0	40.0	41.0	37.0	41.0
22	39.59382762778554	41.0	40.0	41.0	37.0	41.0
23	39.530670341426266	41.0	39.0	41.0	37.0	41.0
24	39.49784115428551	41.0	39.0	41.0	37.0	41.0
25	39.47662213740458	41.0	39.0	41.0	37.0	41.0
26	39.410130822650544	41.0	39.0	41.0	36.0	41.0
27	39.33678446447027	41.0	39.0	41.0	36.0	41.0
28	39.235639426302335	41.0	39.0	41.0	36.0	41.0
29	39.1641278036663	41.0	39.0	41.0	35.0	41.0
30	39.10304096933872	41.0	39.0	41.0	35.0	41.0
31	39.057477425613314	40.0	39.0	41.0	35.0	41.0
32	38.97807836666606	40.0	39.0	41.0	35.0	41.0
33	38.87303720694094	40.0	38.0	41.0	35.0	41.0
34	38.838113769469274	40.0	38.0	41.0	35.0	41.0
35	38.725016318833745	40.0	38.0	41.0	35.0	41.0
36	38.672382413736834	40.0	38.0	41.0	35.0	41.0
37	38.600046916647024	40.0	38.0	41.0	35.0	41.0
38	38.499210123116534	40.0	38.0	41.0	35.0	41.0
39	38.411600650940144	40.0	38.0	41.0	35.0	41.0
40	38.31475517216369	40.0	38.0	41.0	34.0	41.0
41	38.230219080343055	40.0	37.0	41.0	34.0	41.0
42	38.10665037805298	40.0	37.0	41.0	34.0	41.0
43	37.151346983735564	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	1.0
16	1.0
17	6.0
18	15.0
19	33.0
20	78.0
21	137.0
22	244.0
23	401.0
24	621.0
25	934.0
26	1456.0
27	1990.0
28	2712.0
29	3820.0
30	5039.0
31	6769.0
32	8840.0
33	12390.0
34	18654.0
35	27281.0
36	44639.0
37	83796.0
38	195031.0
39	467526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68608456782289	17.40482946818734	11.455481314935358	27.453604649054412
2	20.49271545393556	18.977783721056735	31.955676234338455	28.573824590669254
3	20.35491196895795	20.88980707512103	28.2923247085275	30.462956247393517
4	14.740213232760965	14.901815016953455	33.283281354825846	37.07469039545973
5	16.88557324436547	34.1181483563308	32.22199053507643	16.774287864227304
6	36.18554060669798	33.342777102863046	15.247910282678465	15.22377200776051
7	31.890400899349057	28.404516690540515	19.787719171003246	19.91736323910718
8	29.875024931551557	30.025520842777105	18.73005475875324	21.3693994669181
9	28.04822215372341	13.365011513843811	18.244569454769632	40.34219687766314
10	19.47233504378887	23.8632345741691	29.73835469891752	26.926075683124512
11	39.59844336458087	20.094717230875233	19.13587242298417	21.17096698155972
12	24.4957027071132	23.80283222425704	25.735480770974235	25.965984297655524
13	32.062428605102355	16.84103642726333	23.135799894834182	27.96073507280013
14	24.21590270348679	19.006568330583306	22.021812841108957	34.75571612482094
15	28.27226614204638	25.190613044187778	19.4521631520734	27.084957661692442
16	28.35284038367391	23.31576036699244	21.496550379866186	26.834848869467464
17	26.83156243767112	23.816657902848544	22.374027669489223	26.977751989991116
18	27.22491432612283	21.828026690359195	23.80521205417853	27.14184692933945
19	27.394675527188987	23.08333031132708	22.757520262551903	26.764473898932025
20	27.36237783539736	21.840945767075844	22.97068502837664	27.82599136915015
21	29.05205707965404	22.58435930445504	22.284840710050588	26.07874290584033
22	28.850224837264964	21.962657068774817	22.109186596797883	27.07793149716234
23	27.027048466936233	21.784396475131913	23.060325288752697	28.128229769179164
24	26.76787365596272	23.057378832659424	22.565320665083135	27.609426846294717
25	27.16564522855433	21.887069137458976	22.833448169570815	28.113837464415877
26	27.219021413936282	23.17467045021849	22.756047034505265	26.850261101339957
27	27.91007869304274	22.019772986890537	22.383546989175173	27.686601330891552
28	26.443876810937244	22.496985548766116	23.728944171456547	27.330193468840093
29	25.988536019292486	22.835714674257947	24.00987742742652	27.165871879023047
30	25.57931859803086	23.498667295243965	24.068806549291942	26.85320755743323
31	27.252112382368406	23.116534604993564	22.395446138782617	27.235906873855413
32	26.07239669271636	22.591838769922575	23.00309604540262	28.33266849195844
33	25.61025638701021	22.20369984225127	24.0224565284401	28.163587242298416
34	27.246446120650575	22.081195263911805	23.542977461877392	27.129381153560228
35	27.065012420445683	22.304785951297347	24.206723359503908	26.42347826875306
36	25.64833366575402	23.148945622019546	23.813711446755274	27.38900926547116
37	26.378261500244783	21.814087686533334	24.581716559989847	27.225934253232037
38	25.337255897445193	21.18649253866657	25.799622853620065	27.676628710268176
39	25.77480462729597	20.909072364961652	25.671112037859693	27.645010969882687
40	25.18086707403311	21.18252615546409	26.480367536400067	27.156239234102735
41	24.0697131511668	21.180486301245672	27.622459248245722	27.127341299341808
42	24.828198944715417	21.266386828887963	27.622119272542655	26.283294953853964
43	23.30442784355678	20.340179688491595	28.027256985367444	28.328135482584177
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	10.0
2	11.0
3	49.0
4	87.0
5	87.0
6	119.5
7	152.0
8	153.0
9	154.0
10	216.5
11	279.0
12	279.0
13	527.0
14	775.0
15	1230.0
16	1685.0
17	1655.0
18	1625.0
19	1625.0
20	1862.5
21	2100.0
22	1843.0
23	1586.0
24	1907.5
25	2229.0
26	2229.0
27	2906.5
28	3584.0
29	4851.0
30	6118.0
31	8041.0
32	9964.0
33	9964.0
34	13208.5
35	16453.0
36	21056.0
37	25659.0
38	30521.5
39	35384.0
40	35384.0
41	38911.5
42	42439.0
43	46045.0
44	49651.0
45	52559.5
46	55468.0
47	55468.0
48	60798.5
49	66129.0
50	67516.0
51	68903.0
52	69789.5
53	70676.0
54	70676.0
55	69093.0
56	67510.0
57	65788.5
58	64067.0
59	63543.5
60	63020.0
61	63020.0
62	59548.5
63	56077.0
64	52270.5
65	48464.0
66	43639.5
67	38815.0
68	38815.0
69	33777.0
70	28739.0
71	24953.5
72	21168.0
73	16474.5
74	11781.0
75	11781.0
76	9444.5
77	7108.0
78	6127.0
79	5146.0
80	4483.0
81	3820.0
82	3820.0
83	3038.5
84	2257.0
85	1927.0
86	1597.0
87	1301.5
88	1006.0
89	1006.0
90	785.0
91	564.0
92	331.0
93	98.0
94	72.0
95	46.0
96	46.0
97	28.0
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	882416.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.90278985808907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.49502948144332	44.89333338944573
2	7.166192704572085	7.438907880559517
3	2.0784586144040684	3.236334020764481
4	1.0518274851181737	2.1837112370820355
5	0.5796002535426743	1.5041435080660288
6	0.4040697414527866	1.258340812718182
7	0.283771352413106	1.0309967410430225
8	0.22696958123919816	0.9424283583389262
9	0.1671386686425561	0.7807466876143835
>10	1.2442113971648738	12.956832023343054
>50	0.15718350657200966	5.6974423010949495
>100	0.13193646346293358	13.155317614208526
>500	0.012074052394709217	3.9796268590782238
>1k	0.0015366975775084456	0.9418385666430154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1550	0.17565411325270622	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1256	0.14233649435187032	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1194	0.13531032982176208	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1154	0.13077732044749868	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1061	0.12023807365233632	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1046	0.11853819513698753	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1005	0.11389186052836757	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	886	0.100406157639934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1332523435658465E-4
2	0.0	0.0	0.0	0.0	1.1332523435658465E-4
3	0.0	0.0	0.0	0.0	1.1332523435658465E-4
4	0.0	0.0	0.0	0.0	1.1332523435658465E-4
5	0.0	1.1332523435658465E-4	0.0	0.0	1.1332523435658465E-4
6	0.0	1.1332523435658465E-4	0.0	0.0	1.1332523435658465E-4
7	0.0	1.1332523435658465E-4	0.0	0.0	1.1332523435658465E-4
8	0.0	1.1332523435658465E-4	0.0	0.0	1.1332523435658465E-4
9	0.0	1.1332523435658465E-4	0.0	0.0	1.1332523435658465E-4
10	0.0	1.1332523435658465E-4	0.0	0.0	1.1332523435658465E-4
11	0.0	2.266504687131693E-4	0.0	0.0	1.1332523435658465E-4
12	0.0	2.266504687131693E-4	0.0	0.0	1.1332523435658465E-4
13	0.0	2.266504687131693E-4	0.0	0.0	1.1332523435658465E-4
14	0.0	2.266504687131693E-4	0.0	0.0	1.1332523435658465E-4
15	0.0	2.266504687131693E-4	0.0	0.0	2.266504687131693E-4
16	0.0	2.266504687131693E-4	0.0	5.666261717829233E-4	2.266504687131693E-4
17	0.0	2.266504687131693E-4	0.0	0.0010199271092092618	2.266504687131693E-4
18	0.0	2.266504687131693E-4	0.0	0.0011332523435658465	3.3997570306975396E-4
19	0.0	2.266504687131693E-4	0.0	0.001246577577922431	3.3997570306975396E-4
20	0.0	2.266504687131693E-4	0.0	0.0013599028122790158	3.3997570306975396E-4
21	0.0	2.266504687131693E-4	0.0	0.0016998785153487697	3.3997570306975396E-4
22	0.0	2.266504687131693E-4	0.0	0.002266504687131693	3.3997570306975396E-4
23	0.0	2.266504687131693E-4	0.0	0.0035130822650541243	3.3997570306975396E-4
24	0.0	2.266504687131693E-4	0.0	0.005666261717829232	3.3997570306975396E-4
25	0.0	2.266504687131693E-4	0.0	0.007479465467534587	3.3997570306975396E-4
26	0.0	2.266504687131693E-4	0.0	0.015185581403782343	3.3997570306975396E-4
27	0.0	2.266504687131693E-4	0.0	0.038417254446882196	3.3997570306975396E-4
28	0.0	2.266504687131693E-4	0.0	0.11751826802777827	3.3997570306975396E-4
29	0.0	4.533009374263386E-4	0.0	0.23243005566535513	3.3997570306975396E-4
30	0.0	4.533009374263386E-4	0.0	0.3863257239215971	3.3997570306975396E-4
31	0.0	4.533009374263386E-4	0.0	0.7761645301082483	3.3997570306975396E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAA	20	0.001841704	37.0	1
CCGCTTA	120	0.0	27.749998	25
CGCTTAT	140	0.0	23.785715	26
TTGTCGG	40	0.0019308333	23.125002	22
AGGGTTA	40	0.0019308333	23.125002	23
AGCTATA	40	0.0019308333	23.125002	37
GGTATCA	835	0.0	21.047903	1
GCAGTCG	465	0.0	20.688171	9
AGTCGGT	465	0.0	20.688171	11
GTGATTC	475	0.0	20.252632	16
CAGTCGG	475	0.0	20.252632	10
TCGGTGA	500	0.0	19.980001	13
TATTAGA	130	3.274181E-11	19.923077	2
TCTAGAT	215	0.0	19.790697	2
CTTATTG	485	0.0	19.453608	28
GATATGC	385	0.0	19.22078	34
TTATTGA	495	0.0	19.060606	29
GTTCAAA	350	0.0	19.02857	1
CTAGTAA	60	9.2345005E-4	18.5	30
GGGCAGT	530	0.0	18.5	7
>>END_MODULE