FastQCFastQC Report
Fri 10 Feb 2017
ERR1631383.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631383.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84666
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2310.27283679399050387No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2020.2385845557839038No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1920.22677343916093828No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT1340.15826896274773816No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1270.1500011811116623No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1220.14409562280017954No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC1220.14409562280017954No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT1200.14173339947558644No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1180.13937117615099331No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1070.12637894786573123No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT1030.12165450121654502No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG1020.12047338955424845No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGAC990.1169300545673588No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG980.11574894290506225No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC980.11574894290506225No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT960.11338671958046914No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC940.11102449625587604No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG860.1015756029575036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA200.001832322137.030
ACGCCGC200.001832322137.06
GAATAAC200.001832322137.01
ATCGTTT200.001832322137.029
AACGCCG200.001832322137.05
GCATCGT200.001832322137.027
ATTAGAG303.571629E-430.8333323
TATTCCA303.571629E-430.8333325
GCGAAAG250.005467982529.618
ACCGCAG250.005467982529.629
CGCATCG250.005467982529.613
TAACGCC250.005467982529.64
AGCATTT250.005467982529.623
AAGCATT250.005467982529.622
TATTAGA250.005467982529.62
CATCGTT250.005467982529.628
CATCGCC250.005467982529.615
GGCATCG250.005467982529.626
CATTTGC250.005467982529.625
CGTTTAT250.005467982529.631
Produced by FastQC (version 0.11.5)