FastQCFastQC Report
Fri 10 Feb 2017
ERR1631382.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631382.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314044
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.14456572964297998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4050.12896282049649094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3380.10762823043904675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1700.031.5588231
GGATTAA250.005490336629.66
CGCAAGA705.082462E-621.1428572
CGAACGA450.003819710320.55555516
CGTTCAA450.003819710320.55555529
GAACGAG450.003819710320.55555517
GTATAAT450.003819710320.5555551
TATAATA450.003819710320.5555552
ACCTTGC450.003819710320.5555558
AATACTA555.131661E-420.1818185
GCCCATA609.21645E-418.51
TCGCACG609.21645E-418.522
ATTAGAG500.00702377818.53
GATAACC609.21645E-418.56
CCGATCG609.21645E-418.518
ATAATAC500.00702377818.53
CCGTGTT500.00702377818.537
TCAATCA500.00702377818.55
CGAGCTT500.00702377818.510
TAGATAA609.21645E-418.54
Produced by FastQC (version 0.11.5)