FastQCFastQC Report
Fri 10 Feb 2017
ERR1631381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155216
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2480.15977734254200598No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2050.1320740129883517No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1840.11854447995052056No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1840.11854447995052056No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1840.11854447995052056No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC1610.10372641995670549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTGT250.005481888529.59999810
CACCGTG250.005481888529.5999987
GGTATCA1700.028.2941191
ACTCTAA400.001923711323.12510
TGAACAC400.001923711323.1255
ACACTCT502.687538E-422.1999998
CTCTATG1107.2759576E-1121.8636361
AAGAATT603.6996244E-521.58333216
TCTATGG1201.0913936E-1121.5833322
CATGGGT450.003811139120.55555517
TAGATAA450.003811139120.5555554
CGATCGC450.003811139120.55555519
ATCGCAC450.003811139120.55555521
CTTATAC3000.020.3537
TCTAGCG656.853095E-519.92307728
GCAATAC656.853095E-519.92307737
CTAGCGG656.853095E-519.92307729
GAGCTAT759.181384E-619.73333432
TATGGGC1254.0381565E-1019.244
CTAGATA609.1878365E-418.53
Produced by FastQC (version 0.11.5)