##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631381.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	155216
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.843508401195756	34.0	33.0	34.0	31.0	34.0
2	33.00918719719616	34.0	33.0	34.0	31.0	34.0
3	33.13075327285847	34.0	33.0	34.0	31.0	34.0
4	36.46532573961447	37.0	37.0	37.0	35.0	37.0
5	36.440624677868264	37.0	37.0	37.0	35.0	37.0
6	36.48307519843315	37.0	37.0	37.0	35.0	37.0
7	36.47060870013401	37.0	37.0	37.0	35.0	37.0
8	36.44878105349964	37.0	37.0	37.0	35.0	37.0
9	38.306476136480775	39.0	39.0	39.0	37.0	39.0
10	38.25972837851768	39.0	39.0	39.0	37.0	39.0
11	38.32525641686424	39.0	39.0	39.0	37.0	39.0
12	38.09125992165756	39.0	39.0	39.0	37.0	39.0
13	38.22274121224616	39.0	39.0	39.0	37.0	39.0
14	39.749516802391504	41.0	40.0	41.0	38.0	41.0
15	39.7610684465519	41.0	40.0	41.0	38.0	41.0
16	39.75028347593032	41.0	40.0	41.0	38.0	41.0
17	39.73516905473662	41.0	40.0	41.0	38.0	41.0
18	39.71054530460777	41.0	40.0	41.0	38.0	41.0
19	39.7183666632306	41.0	40.0	41.0	38.0	41.0
20	39.69134625296361	41.0	40.0	41.0	37.0	41.0
21	39.654365529326874	41.0	40.0	41.0	37.0	41.0
22	39.615741933821255	41.0	40.0	41.0	37.0	41.0
23	39.55497500257705	41.0	39.0	41.0	37.0	41.0
24	39.51341356561179	41.0	39.0	41.0	37.0	41.0
25	39.4839256262241	41.0	39.0	41.0	37.0	41.0
26	39.42659906195237	41.0	39.0	41.0	37.0	41.0
27	39.36068446551902	41.0	39.0	41.0	36.0	41.0
28	39.263091433872795	41.0	39.0	41.0	36.0	41.0
29	39.18005875682919	41.0	39.0	41.0	36.0	41.0
30	39.12894289248531	41.0	39.0	41.0	35.0	41.0
31	39.10316591073085	40.0	39.0	41.0	35.0	41.0
32	39.02867616740542	40.0	39.0	41.0	35.0	41.0
33	38.93597309555716	40.0	39.0	41.0	35.0	41.0
34	38.88303396557056	40.0	38.0	41.0	35.0	41.0
35	38.7948214101639	40.0	38.0	41.0	35.0	41.0
36	38.72860400989589	40.0	38.0	41.0	35.0	41.0
37	38.67623183176992	40.0	38.0	41.0	35.0	41.0
38	38.60143928460983	40.0	38.0	41.0	35.0	41.0
39	38.5206937429131	40.0	38.0	41.0	35.0	41.0
40	38.43634676837439	40.0	38.0	41.0	35.0	41.0
41	38.34725157200289	40.0	37.0	41.0	34.0	41.0
42	38.25324708792908	40.0	37.0	41.0	34.0	41.0
43	37.3090016493145	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	7.0
20	8.0
21	24.0
22	35.0
23	65.0
24	126.0
25	151.0
26	204.0
27	256.0
28	424.0
29	608.0
30	845.0
31	1102.0
32	1508.0
33	2120.0
34	3240.0
35	4739.0
36	7814.0
37	14496.0
38	34759.0
39	82680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79252138954747	16.864885063395526	11.967838367178642	27.374755179878363
2	21.122822389444387	18.604396453973816	31.803422327595094	28.469358828986703
3	20.537186887949694	20.072028656839503	27.7722657457994	31.618518709411404
4	15.051927636326152	14.817415730337077	32.287264199567055	37.843392433769715
5	17.49433048139367	33.59576332336873	31.846587980620555	17.06331821461705
6	37.14823214101639	32.87805380888568	14.960442222451292	15.013271827646635
7	33.1087001340068	27.349628904236678	19.128826925059272	20.412844036697248
8	29.960184517060096	30.609602102875993	17.830636016905473	21.59957736315844
9	28.22840428821771	13.54241830739099	17.498196062261623	40.73098134212968
10	19.61846716833316	23.320405112874962	29.112333780022677	27.9487939387692
11	40.25551489537161	19.702221420472117	18.82537882692506	21.216884857231214
12	25.70353571796722	23.21861148335223	24.9922688382641	26.085583960416454
13	32.611328728997016	16.536310689619626	22.71157612617256	28.140784455210806
14	24.850530873105868	18.35571075146892	21.434001649314503	35.35975672611071
15	28.84109885578806	24.23010514379961	19.27314194412947	27.65565405628286
16	28.814039789712403	22.86555509741264	20.78200700958664	27.53839810328832
17	27.329012472940935	23.4215544789197	21.542882177095144	27.70655087104422
18	27.773554272755383	21.03455829295949	23.190263890320587	28.001623543964538
19	28.033836717864137	22.604628388825894	22.055715905576744	27.305818987733222
20	28.319245438614576	21.164699515513867	22.076332336872486	28.43972270899907
21	30.042005978765076	21.997732192557468	21.502937841459644	26.457323987217812
22	29.72502834759303	21.341871971961655	20.964977837336356	27.968121843108957
23	27.77677559014535	21.355401504999485	22.079553654262448	28.788269250592723
24	27.659519637150808	22.24899494897433	21.916554994330482	28.174930419544374
25	27.656298319760847	21.040356664261417	22.11563240903	29.187712606947734
26	28.11243686217916	22.37011648283682	22.127873415111843	27.389573239872178
27	28.62011648283682	21.405009792804865	21.86436965261313	28.11050407174518
28	27.26587465209772	21.792856406556023	22.703200700958664	28.238068240387587
29	26.44766003504793	22.18843418204309	23.62514173796516	27.73876404494382
30	26.264689207298215	23.005360272136894	23.44925780847335	27.280692712091536
31	27.49845376765282	22.477064220183486	21.866302443047108	28.158179569116587
32	26.247938356870428	21.70910215441707	22.88359447479641	29.159365013916087
33	26.03082156478714	21.20593237810535	23.794583032677043	28.96866302443047
34	27.328368209462944	20.946938459952584	23.903463560457684	27.82122977012679
35	27.300020616431297	20.792959488712505	24.537418822801772	27.36960107205443
36	25.487707452839913	21.53515101535924	24.716524069683537	28.26061746211731
37	25.85236058138336	19.767936295227294	25.62364704669622	28.756056076693127
38	24.623750128852695	19.16104009895887	27.05777754870632	29.157432223482115
39	24.85504071745181	18.60117513658386	28.16333367694052	28.38045046902381
40	24.10576229254716	19.349809298010516	28.712890423667663	27.831537985774663
41	22.633620245335532	19.18101226677662	30.514251108133184	27.67111637975466
42	22.71479744356252	19.55983919183589	30.406659107308524	27.318704257293064
43	21.010076280795793	18.830532934748994	30.937532213173903	29.221858571281313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	4.0
4	7.0
5	7.0
6	5.0
7	3.0
8	8.0
9	13.0
10	20.0
11	27.0
12	27.0
13	43.5
14	60.0
15	109.0
16	158.0
17	130.0
18	102.0
19	102.0
20	143.0
21	184.0
22	145.5
23	107.0
24	145.5
25	184.0
26	184.0
27	249.0
28	314.0
29	461.5
30	609.0
31	872.0
32	1135.0
33	1135.0
34	1650.5
35	2166.0
36	2854.5
37	3543.0
38	4309.0
39	5075.0
40	5075.0
41	5747.5
42	6420.0
43	7437.5
44	8455.0
45	9103.0
46	9751.0
47	9751.0
48	10773.5
49	11796.0
50	12445.0
51	13094.0
52	13372.5
53	13651.0
54	13651.0
55	13261.0
56	12871.0
57	12710.0
58	12549.0
59	12364.5
60	12180.0
61	12180.0
62	11462.5
63	10745.0
64	9968.0
65	9191.0
66	8164.5
67	7138.0
68	7138.0
69	6026.0
70	4914.0
71	4210.0
72	3506.0
73	2705.5
74	1905.0
75	1905.0
76	1539.5
77	1174.0
78	991.5
79	809.0
80	717.5
81	626.0
82	626.0
83	468.0
84	310.0
85	256.5
86	203.0
87	167.5
88	132.0
89	132.0
90	107.0
91	82.0
92	47.0
93	12.0
94	10.5
95	9.0
96	9.0
97	5.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	155216.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.8079166627857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.13483738028735	58.657779500729056
2	5.393462612097277	7.0986507620149615
3	1.7059590005894965	3.367968232227683
4	0.829943951245397	2.184675295133607
5	0.535962975103705	1.7635303399981654
6	0.39297283137499806	1.5516434002718886
7	0.2679814722702516	1.2344711676031932
8	0.19698638072224578	1.037061066101866
9	0.17798769425620756	1.0541699415552501
>10	1.2479137215265552	15.742797554160736
>50	0.09199363972791912	4.14363802869713
>100	0.023998340798587592	2.1636147115064577
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	248	0.15977734254200598	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	205	0.1320740129883517	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	184	0.11854447995052056	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	184	0.11854447995052056	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	184	0.11854447995052056	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	161	0.10372641995670549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	6.442634779919596E-4	0.0	0.0	0.0
10	0.0	6.442634779919596E-4	0.0	0.0	0.0
11	0.0	6.442634779919596E-4	0.0	0.0	0.0
12	0.0	6.442634779919596E-4	0.0	0.0	0.0
13	0.0	6.442634779919596E-4	0.0	0.0	0.0
14	0.0	6.442634779919596E-4	0.0	0.0	0.0
15	0.0	6.442634779919596E-4	0.0	0.0	0.0
16	0.0	6.442634779919596E-4	0.0	0.0	0.0
17	0.0	6.442634779919596E-4	0.0	6.442634779919596E-4	0.0
18	0.0	6.442634779919596E-4	0.0	0.001288526955983919	0.0
19	0.0	6.442634779919596E-4	0.0	0.001288526955983919	0.0
20	0.0	6.442634779919596E-4	0.0	0.0019327904339758789	0.0
21	0.0	6.442634779919596E-4	0.0	0.0019327904339758789	0.0
22	0.0	6.442634779919596E-4	0.0	0.003221317389959798	0.0
23	0.0	6.442634779919596E-4	0.0	0.004509844345943717	0.0
24	0.0	6.442634779919596E-4	0.0	0.005154107823935676	0.0
25	0.0	6.442634779919596E-4	0.0	0.006442634779919596	0.0
26	0.0	6.442634779919596E-4	0.0	0.017395113905782907	0.0
27	0.0	6.442634779919596E-4	0.0	0.04896402432738893	0.0
28	0.0	6.442634779919596E-4	0.0	0.22742500773116173	0.0
29	0.0	6.442634779919596E-4	0.0	0.4844861354499536	0.0
30	0.0	6.442634779919596E-4	0.0	0.8066178744459334	0.0
31	0.0	6.442634779919596E-4	0.0	1.8561230800948356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTTGT	25	0.0054818885	29.599998	10
CACCGTG	25	0.0054818885	29.599998	7
GGTATCA	170	0.0	28.294119	1
ACTCTAA	40	0.0019237113	23.125	10
TGAACAC	40	0.0019237113	23.125	5
ACACTCT	50	2.687538E-4	22.199999	8
CTCTATG	110	7.2759576E-11	21.863636	1
AAGAATT	60	3.6996244E-5	21.583332	16
TCTATGG	120	1.0913936E-11	21.583332	2
CATGGGT	45	0.0038111391	20.555555	17
TAGATAA	45	0.0038111391	20.555555	4
CGATCGC	45	0.0038111391	20.555555	19
ATCGCAC	45	0.0038111391	20.555555	21
CTTATAC	300	0.0	20.35	37
TCTAGCG	65	6.853095E-5	19.923077	28
GCAATAC	65	6.853095E-5	19.923077	37
CTAGCGG	65	6.853095E-5	19.923077	29
GAGCTAT	75	9.181384E-6	19.733334	32
TATGGGC	125	4.0381565E-10	19.24	4
CTAGATA	60	9.1878365E-4	18.5	3
>>END_MODULE