FastQCFastQC Report
Fri 10 Feb 2017
ERR1631380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4196057
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA80850.19268089065520322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72370.17247144164152203No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC65420.1559082729333753No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC64240.15309610903760362No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT63960.1524288159097934No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC60030.14306288022302843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55130.13138525048634944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53880.1284062633086252No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT52670.12552260372058816No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC52370.12480764679793435No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC50750.12094687941560374No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC48920.11658564218741548No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC46110.1098888790118914No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA44760.10667157285994923No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG43090.10269164599050966No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA42900.10223883993949559No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG42020.10014163296637772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41100.021.0656931
ATACACA13450.018.7063237
GCAGTCG15050.018.3156159
AGTCGGT14950.018.19063611
CTTATAC71450.018.02099237
CAAGACG18800.017.6143634
TATACAC21900.017.57077637
CGGTGAT15650.017.49520914
CGCCTTA11500.017.21304325
TCGGTGA15700.017.20382113
ACGGACC18600.016.9086028
AAGACGG19200.016.669275
AGACGGA19000.016.4552616
CAGTCGG16650.016.44444510
GGCAGTC16950.016.3716818
GACGGAC19450.016.35997
CTCTATG23350.016.32121
TCGTTTA14000.016.12142830
GCCTTAT12400.016.11290226
GTGATTC17050.016.05865116
Produced by FastQC (version 0.11.5)