##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631380.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4196057
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84703210657052	34.0	33.0	34.0	31.0	34.0
2	33.019061704833845	34.0	33.0	34.0	31.0	34.0
3	33.13400127786634	34.0	33.0	34.0	31.0	34.0
4	36.45690275418089	37.0	37.0	37.0	35.0	37.0
5	36.44402018371056	37.0	37.0	37.0	35.0	37.0
6	36.489032441647005	37.0	37.0	37.0	35.0	37.0
7	36.46979032934967	37.0	37.0	37.0	35.0	37.0
8	36.455226656835215	37.0	37.0	37.0	35.0	37.0
9	38.30849843078872	39.0	39.0	39.0	37.0	39.0
10	38.26935310935957	39.0	39.0	39.0	37.0	39.0
11	38.32632778820688	39.0	39.0	39.0	37.0	39.0
12	38.10327576579632	39.0	39.0	39.0	37.0	39.0
13	38.230255690044245	39.0	39.0	39.0	37.0	39.0
14	39.75981236670522	41.0	40.0	41.0	38.0	41.0
15	39.76900266130799	41.0	40.0	41.0	38.0	41.0
16	39.757644617315734	41.0	40.0	41.0	38.0	41.0
17	39.74269534470099	41.0	40.0	41.0	38.0	41.0
18	39.72120540783884	41.0	40.0	41.0	38.0	41.0
19	39.731765083267454	41.0	40.0	41.0	38.0	41.0
20	39.71039978722882	41.0	40.0	41.0	38.0	41.0
21	39.67712140230697	41.0	40.0	41.0	37.0	41.0
22	39.62576795310454	41.0	40.0	41.0	37.0	41.0
23	39.556560599629606	41.0	39.0	41.0	37.0	41.0
24	39.520795117892824	41.0	39.0	41.0	37.0	41.0
25	39.498572111865975	41.0	39.0	41.0	37.0	41.0
26	39.43412279671129	41.0	39.0	41.0	37.0	41.0
27	39.36350912296949	41.0	39.0	41.0	36.0	41.0
28	39.25492289547068	41.0	39.0	41.0	36.0	41.0
29	39.172833209844384	41.0	39.0	41.0	36.0	41.0
30	39.1119963337009	41.0	39.0	41.0	35.0	41.0
31	39.0708188663786	40.0	39.0	41.0	35.0	41.0
32	38.99316739500917	40.0	39.0	41.0	35.0	41.0
33	38.88165604042081	40.0	38.0	41.0	35.0	41.0
34	38.83138003130082	40.0	38.0	41.0	35.0	41.0
35	38.71903932668217	40.0	38.0	41.0	35.0	41.0
36	38.6651913927766	40.0	38.0	41.0	35.0	41.0
37	38.60330448323271	40.0	38.0	41.0	35.0	41.0
38	38.50924784863504	40.0	38.0	41.0	35.0	41.0
39	38.42271256086369	40.0	38.0	41.0	35.0	41.0
40	38.315727836871616	40.0	38.0	41.0	34.0	41.0
41	38.22433417849186	40.0	37.0	41.0	34.0	41.0
42	38.09954535889288	40.0	37.0	41.0	34.0	41.0
43	37.14250497550439	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	4.0
13	4.0
14	4.0
15	7.0
16	26.0
17	38.0
18	90.0
19	156.0
20	319.0
21	596.0
22	1073.0
23	1858.0
24	2979.0
25	4344.0
26	6508.0
27	9331.0
28	12841.0
29	17716.0
30	24030.0
31	31632.0
32	41882.0
33	57003.0
34	86923.0
35	127422.0
36	212050.0
37	403790.0
38	916426.0
39	2236999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.470868722708005	16.89023766836342	11.743405773563133	26.89548783536544
2	20.204253660043225	19.34966565039512	32.34434136619212	28.101739323369536
3	19.89451048925217	20.663303668181822	28.768508149436485	30.673677693129527
4	14.329452626596826	15.034495479923176	33.32921835904517	37.30683353443483
5	16.574012221473637	33.89532125040246	32.25335118183571	17.277315346288194
6	35.68087850093552	33.65247421567438	15.757579079597821	14.909068203792275
7	31.592635657713895	29.090929889655932	19.688984205886623	19.62745024674355
8	29.43899475150123	30.886019899157706	18.947049575351336	20.727935773989724
9	27.91256172163534	14.031673068311513	18.238670256385937	39.817094953667215
10	19.06611373487062	23.670936786607047	29.604983917044024	27.65796556147831
11	39.05838266734698	20.371911058405544	19.73147647898968	20.83822979525779
12	24.808814560908015	23.783089695874008	26.065279856779828	25.342815886438146
13	31.217688415576816	16.99216669363643	24.125983035978777	27.66416185480798
14	24.335060272060176	18.912278837012938	22.460276397579918	34.292384493346965
15	27.29531557841087	25.322034471886347	20.19729474599606	27.185355203706717
16	27.67781753203067	23.83985727553272	21.808807649657762	26.673517542778853
17	26.437915404866995	24.132465312077507	22.74037268797826	26.689246595077236
18	27.03395115938606	21.983376298272404	24.25913184687434	26.723540695467197
19	26.787886818506042	23.452493614838886	23.305641462925788	26.453978103729288
20	27.2483190766951	22.04259856336556	23.05905282030249	27.650029539636854
21	29.32800960520794	22.627337998506693	22.655912443515426	25.388739952769946
22	28.722107445156254	22.150795377660504	22.512396757241383	26.614700419941865
23	26.775184417180224	22.039452752905884	23.40568776830248	27.779675061611414
24	26.309032503609934	23.36572167632613	23.297038147956524	27.028207672107406
25	26.700423754968057	22.075748732679276	23.210838174981895	28.012989337370776
26	26.701710677428835	23.657376436974044	23.503922849475114	26.136990036122004
27	27.6658300876275	22.13080041572362	22.976499127633396	27.226870369015483
28	26.178195386764287	22.816372608856362	24.049291990075446	26.9561400143039
29	25.61299810750903	23.012747443611943	24.462751578446145	26.911502870432884
30	25.130235361435748	23.844409167940285	24.6428015634678	26.38255390715617
31	26.705809763785382	23.250971090240196	22.749738623665028	27.293480522309398
32	25.73873519830641	22.466091380550836	23.561310058466795	28.233863362675958
33	25.238384511935845	22.134613519311106	24.622306131685058	28.004695837067988
34	26.807643461468704	21.959306081876388	24.477765673821878	26.755284782833026
35	26.600854087539805	21.819460507805307	25.232593360862353	26.347092043792543
36	24.80049722870781	22.901309491267636	24.950471359183158	27.3477219208414
37	25.712710766321813	21.441009976747218	25.485711943379226	27.360567313551748
38	24.55340811623865	20.680653289504885	26.872442390558565	27.8934962036979
39	25.213956817078508	20.240621135508885	26.930735211652273	27.61468683576033
40	24.407175593658522	20.942065372324542	27.660158096041116	26.990600937975817
41	23.08140714008413	21.038989699138977	29.068241923310385	26.811361237466507
42	24.032728821367296	21.178978264594594	28.767245058873126	26.02104785516498
43	22.285922236042076	20.109807850560657	29.05611148752269	28.548158425874576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	77.5
2	98.0
3	453.5
4	809.0
5	809.0
6	1089.5
7	1370.0
8	1354.0
9	1338.0
10	1983.5
11	2629.0
12	2629.0
13	4454.5
14	6280.0
15	10078.5
16	13877.0
17	13091.5
18	12306.0
19	12306.0
20	13560.5
21	14815.0
22	13191.5
23	11568.0
24	13648.5
25	15729.0
26	15729.0
27	19387.5
28	23046.0
29	29326.5
30	35607.0
31	44332.0
32	53057.0
33	53057.0
34	68593.0
35	84129.0
36	105478.0
37	126827.0
38	150233.0
39	173639.0
40	173639.0
41	187841.0
42	202043.0
43	215933.0
44	229823.0
45	247546.0
46	265269.0
47	265269.0
48	286903.5
49	308538.0
50	315308.0
51	322078.0
52	331907.0
53	341736.0
54	341736.0
55	332355.0
56	322974.0
57	315484.0
58	307994.0
59	304045.0
60	300096.0
61	300096.0
62	279716.5
63	259337.0
64	241451.0
65	223565.0
66	200499.5
67	177434.0
68	177434.0
69	152113.0
70	126792.0
71	108831.0
72	90870.0
73	70266.5
74	49663.0
75	49663.0
76	40008.5
77	30354.0
78	25296.5
79	20239.0
80	17853.5
81	15468.0
82	15468.0
83	12355.0
84	9242.0
85	8276.5
86	7311.0
87	6095.0
88	4879.0
89	4879.0
90	3720.0
91	2561.0
92	1482.5
93	404.0
94	268.5
95	133.0
96	133.0
97	95.0
98	57.0
99	36.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4196057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.31983572765524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.59711728956573	41.36038646192654
2	8.445402229593837	8.161608967758948
3	2.3283823152181116	3.375211529475501
4	1.0527672763543117	2.0347816741156546
5	0.5804901538170874	1.402459443698149
6	0.35003082420430875	1.014805915510078
7	0.24281355385318742	0.8212897724243927
8	0.18275405248155124	0.7064516635577472
9	0.13487206158572335	0.5865296274065116
>10	0.8558138960303496	7.903868163835933
>50	0.09958627186614245	3.366358744420369
>100	0.0995690347504792	10.237439881576455
>500	0.01633562414269067	5.41297580556004
>1k	0.013522540810546562	12.014102981944859
>5k	5.428757259708474E-4	1.6017293667889847
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	8085	0.19268089065520322	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7237	0.17247144164152203	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	6542	0.1559082729333753	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	6424	0.15309610903760362	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6396	0.1524288159097934	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	6003	0.14306288022302843	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5513	0.13138525048634944	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5388	0.1284062633086252	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	5267	0.12552260372058816	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	5237	0.12480764679793435	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	5075	0.12094687941560374	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	4892	0.11658564218741548	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	4611	0.1098888790118914	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	4476	0.10667157285994923	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	4309	0.10269164599050966	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	4290	0.10223883993949559	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	4202	0.10014163296637772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.3831897421793842E-5	0.0	0.0	2.3831897421793842E-5
6	0.0	2.3831897421793842E-5	0.0	0.0	2.3831897421793842E-5
7	0.0	2.3831897421793842E-5	0.0	0.0	2.3831897421793842E-5
8	0.0	2.3831897421793842E-5	0.0	0.0	2.3831897421793842E-5
9	0.0	2.3831897421793842E-5	0.0	2.3831897421793842E-5	4.7663794843587684E-5
10	0.0	2.3831897421793842E-5	0.0	1.1915948710896921E-4	4.7663794843587684E-5
11	0.0	2.3831897421793842E-5	0.0	1.668232819525569E-4	4.7663794843587684E-5
12	0.0	2.3831897421793842E-5	0.0	1.9065517937435074E-4	4.7663794843587684E-5
13	0.0	2.3831897421793842E-5	0.0	1.9065517937435074E-4	7.149569226538153E-5
14	0.0	4.7663794843587684E-5	0.0	2.3831897421793842E-4	9.532758968717537E-5
15	0.0	4.7663794843587684E-5	0.0	2.6215087163973224E-4	9.532758968717537E-5
16	0.0	4.7663794843587684E-5	0.0	4.7663794843587684E-4	1.4299138453076305E-4
17	0.0	7.149569226538153E-5	0.0	5.719655381230522E-4	1.4299138453076305E-4
18	0.0	9.532758968717537E-5	0.0	6.672931278102276E-4	1.4299138453076305E-4
19	0.0	9.532758968717537E-5	0.0	8.579483071845783E-4	1.4299138453076305E-4
20	0.0	9.532758968717537E-5	0.0	0.0012392586659332797	1.4299138453076305E-4
21	0.0	1.1915948710896921E-4	0.0	0.0016205690246819812	1.4299138453076305E-4
22	0.0	1.1915948710896921E-4	0.0	0.0024546854344447656	1.4299138453076305E-4
23	0.0	1.1915948710896921E-4	0.0	0.00390843117717419	1.4299138453076305E-4
24	0.0	1.1915948710896921E-4	0.0	0.006768258867789451	1.4299138453076305E-4
25	0.0	1.1915948710896921E-4	0.0	0.00955659086613933	1.4299138453076305E-4
26	0.0	1.1915948710896921E-4	0.0	0.014823440196355769	1.4299138453076305E-4
27	0.0	1.1915948710896921E-4	0.0	0.04835491986881971	1.4299138453076305E-4
28	0.0	1.1915948710896921E-4	0.0	0.2120562232591216	1.9065517937435074E-4
29	0.0	1.1915948710896921E-4	0.0	0.45290137860377017	1.9065517937435074E-4
30	0.0	1.1915948710896921E-4	0.0	0.7900512314298876	1.9065517937435074E-4
31	0.0	1.1915948710896921E-4	0.0	1.6127521623276329	1.9065517937435074E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4110	0.0	21.065693	1
ATACACA	1345	0.0	18.70632	37
GCAGTCG	1505	0.0	18.315615	9
AGTCGGT	1495	0.0	18.190636	11
CTTATAC	7145	0.0	18.020992	37
CAAGACG	1880	0.0	17.614363	4
TATACAC	2190	0.0	17.570776	37
CGGTGAT	1565	0.0	17.495209	14
CGCCTTA	1150	0.0	17.213043	25
TCGGTGA	1570	0.0	17.203821	13
ACGGACC	1860	0.0	16.908602	8
AAGACGG	1920	0.0	16.66927	5
AGACGGA	1900	0.0	16.455261	6
CAGTCGG	1665	0.0	16.444445	10
GGCAGTC	1695	0.0	16.371681	8
GACGGAC	1945	0.0	16.3599	7
CTCTATG	2335	0.0	16.3212	1
TCGTTTA	1400	0.0	16.121428	30
GCCTTAT	1240	0.0	16.112902	26
GTGATTC	1705	0.0	16.058651	16
>>END_MODULE