FastQCFastQC Report
Fri 10 Feb 2017
ERR1631379.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631379.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227808
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37951.6658765276021912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33011.4490272510184015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25811.1329716252282624No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA20910.9178782132321955No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC13550.5947991290911645No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC12550.5509025143980897No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA12010.5271983424638291No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA10920.47935103244837757No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA10490.4604754881303553No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT9680.4249192302289647No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT9330.4095554150863885No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA8170.35863534204242165No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.35863534204242165No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT7860.3450273914875685No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA7270.3191283888186543No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT7230.3173725242309313No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT7170.3147387273493468No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG6790.2980580137659784No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA5530.24274827925270404No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC5400.23704171934260432No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC5180.2273844641101278No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA4990.2190441073184436No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT3920.1720747295968535No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC3870.16987989886219976No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT3780.16592920353982302No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT3720.16329540665823852No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT3650.16022264362972327No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT3560.15627194830734653No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA3530.15495504986655428No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT3510.1540771175726928No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT3460.15188228683803906No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT3460.15188228683803906No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA3430.15056538839724679No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA3390.1488095238095238No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT3310.14529779463407783No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3300.1448588284871471No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA3260.14310296389942406ABI Solid3 Adapter B (100% over 21bp)
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT3250.14266399775249333No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG3240.14222503160556257No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT3190.14003020087090884No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.13739640398932434No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC3110.13651847169546286ABI Solid3 Adapter B (100% over 23bp)
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT2900.12730018260991713No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA2720.11939879196516363No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC2700.11852085967130216No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT2690.1180818935243714No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2660.11676499508357915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTT251.2305204E-437.023
AGGTAGC352.3799716E-531.7142857
TAGAGAC352.3799716E-531.71428533
AAGGTAG352.3799716E-531.7142856
TCGCTTA303.5905043E-430.83333224
TAGGCTG303.5905043E-430.83333233
ATGCCCC3050.029.72131237
GATACAA250.005487210629.65
TCGGGTA405.9195612E-527.7525
CTAAGGT405.9195612E-527.754
TAAGGTA405.9195612E-527.755
ATATGCT604.290814E-827.74999835
ATTGACT1800.025.69444531
TATTGAC1850.025.030
GTCGGGT451.3186673E-424.66666624
TGACTGT1900.024.34210633
TATGCTG701.9088839E-723.78571336
TCCTAAG551.893991E-523.5454542
TTGACTG2050.023.46341332
TCCTGAT400.00192646323.1252
Produced by FastQC (version 0.11.5)