##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631379.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	227808
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75328785644051	34.0	31.0	34.0	31.0	34.0
2	32.89098275740975	34.0	33.0	34.0	31.0	34.0
3	32.8969395280236	34.0	33.0	34.0	31.0	34.0
4	36.34161662452592	37.0	37.0	37.0	35.0	37.0
5	36.346576941986235	37.0	37.0	37.0	35.0	37.0
6	36.44119609495716	37.0	37.0	37.0	35.0	37.0
7	36.42196059839865	37.0	37.0	37.0	35.0	37.0
8	36.42771983424638	37.0	37.0	37.0	35.0	37.0
9	38.25075063211125	39.0	39.0	39.0	37.0	39.0
10	38.22955734653743	39.0	39.0	39.0	37.0	39.0
11	38.26539015311139	39.0	39.0	39.0	37.0	39.0
12	38.06635412277005	39.0	39.0	39.0	37.0	39.0
13	38.17944058154235	39.0	39.0	39.0	37.0	39.0
14	39.560054958561594	41.0	39.0	41.0	37.0	41.0
15	39.5416183803905	41.0	39.0	41.0	37.0	41.0
16	39.427434506250876	40.0	39.0	41.0	37.0	41.0
17	39.3972248560191	40.0	39.0	41.0	37.0	41.0
18	39.57922022053659	41.0	39.0	41.0	37.0	41.0
19	39.61010148897317	41.0	39.0	41.0	37.0	41.0
20	39.65465655288664	41.0	40.0	41.0	37.0	41.0
21	39.644893945778904	41.0	40.0	41.0	37.0	41.0
22	39.57738095238095	41.0	40.0	41.0	37.0	41.0
23	39.531877721590114	41.0	39.0	41.0	37.0	41.0
24	39.50485935524652	41.0	39.0	41.0	37.0	41.0
25	39.490759762607105	41.0	39.0	41.0	37.0	41.0
26	39.38177763730861	41.0	39.0	41.0	36.0	41.0
27	39.294726260710775	41.0	39.0	41.0	35.0	41.0
28	39.174102753195676	41.0	39.0	41.0	35.0	41.0
29	39.026272123893804	40.0	39.0	41.0	35.0	41.0
30	38.86086529006883	40.0	38.0	41.0	35.0	41.0
31	38.65402005197359	40.0	38.0	41.0	35.0	41.0
32	38.480448447815704	40.0	37.0	41.0	35.0	41.0
33	38.32458912768647	40.0	37.0	41.0	35.0	41.0
34	38.22504477454699	40.0	37.0	41.0	35.0	41.0
35	38.086287575502176	40.0	37.0	41.0	35.0	41.0
36	37.967661363955614	40.0	36.0	41.0	34.0	41.0
37	37.81625755021773	40.0	36.0	41.0	34.0	41.0
38	37.65622805169265	40.0	35.0	41.0	33.0	41.0
39	37.450120276724256	40.0	35.0	41.0	33.0	41.0
40	37.306253511729174	40.0	35.0	41.0	33.0	41.0
41	37.055441424357355	39.0	35.0	41.0	33.0	41.0
42	36.8475690054783	39.0	35.0	41.0	33.0	41.0
43	35.83618222362691	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	2.0
13	1.0
14	2.0
15	1.0
16	1.0
17	0.0
18	4.0
19	6.0
20	17.0
21	30.0
22	71.0
23	113.0
24	205.0
25	302.0
26	470.0
27	674.0
28	1066.0
29	1459.0
30	1935.0
31	2368.0
32	3041.0
33	3936.0
34	5508.0
35	8020.0
36	13839.0
37	32749.0
38	51392.0
39	100589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.555502879617926	12.529849697991292	16.009534344711334	26.90511307767945
2	21.098907852226436	18.564317319848293	34.227946340778196	26.108828487147072
3	19.106440511307767	21.54138572833263	31.211370979070097	28.14080278128951
4	12.466199606686331	18.289085545722713	32.56733740693918	36.677377440651775
5	14.358582666104791	38.69135412277005	29.96031746031746	16.989745750807696
6	29.04814580699536	29.448043966849273	21.847345132743364	19.656465093411995
7	31.78729456384324	31.458947885939036	16.355878634639698	20.39787891557803
8	37.025477595167864	26.69572622559348	16.05957648546144	20.219219693777216
9	32.30307978648687	11.997383761764292	14.871295125719906	40.82824132602894
10	21.96806082314932	24.935471976401182	21.172215198763872	31.924252001685634
11	34.66998525073746	26.71723556679309	13.535521140609635	25.077258041859814
12	26.398546144121365	28.9572798145807	23.126492484899565	21.517681556398372
13	37.34372805169265	17.403690827363395	22.864429695181908	22.388151425762047
14	24.94688509622138	20.747734934681837	22.801218570023877	31.504161399072906
15	36.59572973732266	21.280200870908835	18.073553167579718	24.05051622418879
16	26.62285784520298	22.993046776232617	24.77656623121225	25.607529147352153
17	20.811385025986795	29.446288102261555	24.28975277426605	25.452574097485602
18	26.17818513836213	19.742941424357355	26.54428290490237	27.534590532378147
19	24.826608371962354	29.18115254951538	26.34894296951819	19.643296109004073
20	28.59118204804045	18.640697429414242	30.087178676780447	22.680941845764853
21	32.94397036100576	17.79876035960107	26.45034414945919	22.80692512993398
22	30.881268436578168	19.109952240483214	21.577380952380953	28.431398370557666
23	25.532026970080068	22.432925972748983	23.014995083579155	29.020051973591798
24	24.582104228121928	21.91055625790139	28.778620592779884	24.728718921196798
25	23.46449641803624	21.233670459334174	24.497822727911224	30.80401039471836
26	28.70048461862621	19.658659924146647	24.18527883129653	27.455576625930604
27	25.51841901952521	19.962424497822727	23.086107599381936	31.43304888327012
28	23.02948096642787	21.154656552886642	27.190002107037504	28.62586037364798
29	23.764749262536874	21.58045371540947	30.128441494591936	24.52635552746172
30	22.56724961370979	23.344658659924146	31.885184014608793	22.20290771175727
31	23.667298777918248	24.902110549234443	25.823939457789013	25.606651215058296
32	21.98518050287962	23.647545301306362	31.722766540244418	22.6445076555696
33	24.763836212951258	20.763976682118276	31.360619469026545	23.111567635903917
34	25.69312754600365	23.466252282623966	24.5930783817952	26.247541789577188
35	22.825800674252	25.690054782975135	26.211546565528863	25.272597977243993
36	19.56384323640961	24.8301201011378	28.862024160696727	26.744012501755865
37	21.224452170248632	26.068443601629443	28.10744135412277	24.599662873999158
38	21.95620873718219	20.62570234583509	31.23200238797584	26.186086529006886
39	23.316564826520576	20.647211687034698	30.32334246382919	25.712881022615537
40	21.863147914032872	19.781131479140328	28.225084281500212	30.13063632532659
41	17.50728683803905	22.288067144261834	32.47910521140609	27.72554080629302
42	18.760096221379406	20.396123050990308	31.713109285011942	29.130671442618343
43	16.616624525916563	23.942969518190758	34.20950976260711	25.230896193285574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	17.5
2	29.0
3	118.0
4	207.0
5	207.0
6	300.0
7	393.0
8	370.0
9	347.0
10	505.0
11	663.0
12	663.0
13	1368.0
14	2073.0
15	4061.0
16	6049.0
17	5221.0
18	4393.0
19	4393.0
20	5050.0
21	5707.0
22	3679.5
23	1652.0
24	1542.0
25	1432.0
26	1432.0
27	1427.5
28	1423.0
29	1499.5
30	1576.0
31	1543.0
32	1510.0
33	1510.0
34	1592.0
35	1674.0
36	1763.0
37	1852.0
38	2199.0
39	2546.0
40	2546.0
41	3433.5
42	4321.0
43	6408.5
44	8496.0
45	12040.5
46	15585.0
47	15585.0
48	23428.5
49	31272.0
50	30139.5
51	29007.0
52	23141.5
53	17276.0
54	17276.0
55	13932.0
56	10588.0
57	10572.0
58	10556.0
59	10697.5
60	10839.0
61	10839.0
62	10972.5
63	11106.0
64	10969.5
65	10833.0
66	10227.5
67	9622.0
68	9622.0
69	8954.0
70	8286.0
71	7403.5
72	6521.0
73	5469.0
74	4417.0
75	4417.0
76	3618.5
77	2820.0
78	2169.5
79	1519.0
80	1140.0
81	761.0
82	761.0
83	527.0
84	293.0
85	202.0
86	111.0
87	73.0
88	35.0
89	35.0
90	23.0
91	11.0
92	6.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	227808.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.67224640823975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.5146287053071	43.83890583672694
2	7.795519601925271	7.9003298029804085
3	1.933195784755057	2.9387811948143594
4	0.9447526153693174	1.9149094928329204
5	0.539700428196152	1.367391654209396
6	0.3741309827180942	1.1374834407148893
7	0.23776854071948278	0.8433786258425653
8	0.20691882497697625	0.8388033348589421
9	0.18005711624739643	0.8211508705840627
>10	1.0186619487162702	10.223769126552162
>50	0.13230666012563064	4.719205184015242
>100	0.1014682656602581	10.098756318795274
>500	0.011937443018853893	4.424821951002541
>1k	0.008953082264140419	8.932313166070294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3795	1.6658765276021912	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3301	1.4490272510184015	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2581	1.1329716252282624	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2091	0.9178782132321955	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	1355	0.5947991290911645	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	1255	0.5509025143980897	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	1201	0.5271983424638291	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	1092	0.47935103244837757	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	1049	0.4604754881303553	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	968	0.4249192302289647	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	933	0.4095554150863885	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	817	0.35863534204242165	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.35863534204242165	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	786	0.3450273914875685	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	727	0.3191283888186543	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	723	0.3173725242309313	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	717	0.3147387273493468	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	679	0.2980580137659784	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	553	0.24274827925270404	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	540	0.23704171934260432	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	518	0.2273844641101278	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	499	0.2190441073184436	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	392	0.1720747295968535	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	387	0.16987989886219976	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	378	0.16592920353982302	No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT	372	0.16329540665823852	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	365	0.16022264362972327	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	356	0.15627194830734653	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	353	0.15495504986655428	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	351	0.1540771175726928	No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT	346	0.15188228683803906	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	346	0.15188228683803906	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	343	0.15056538839724679	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	339	0.1488095238095238	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	331	0.14529779463407783	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	330	0.1448588284871471	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	326	0.14310296389942406	ABI Solid3 Adapter B (100% over 21bp)
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	325	0.14266399775249333	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	324	0.14222503160556257	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	319	0.14003020087090884	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	313	0.13739640398932434	No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC	311	0.13651847169546286	ABI Solid3 Adapter B (100% over 23bp)
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	290	0.12730018260991713	No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA	272	0.11939879196516363	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC	270	0.11852085967130216	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	269	0.1180818935243714	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	266	0.11676499508357915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	4.389661469307487E-4
12	0.0	0.0	0.0	4.389661469307487E-4	8.779322938614974E-4
13	0.0	0.0	0.0	0.0013168984407922462	8.779322938614974E-4
14	0.0	0.0	0.0	0.0013168984407922462	0.0013168984407922462
15	0.0	0.0	0.0	0.0017558645877229948	0.0013168984407922462
16	0.0	0.0	0.0	0.008779322938614975	0.0017558645877229948
17	0.0	0.0	0.0	0.014924848995645456	0.0017558645877229948
18	0.0	0.0	0.0	0.01668071358336845	0.0017558645877229948
19	0.0	0.0	0.0	0.019753476611883692	0.0017558645877229948
20	0.0	0.0	0.0	0.02326520578732968	0.0017558645877229948
21	0.0	0.0	0.0	0.032483494872875406	0.0017558645877229948
22	0.0	0.0	0.0	0.0496031746031746	0.0017558645877229948
23	0.0	0.0	0.0	0.07813597415367327	0.0017558645877229948
24	0.0	0.0	0.0	0.12115465655288664	0.0017558645877229948
25	0.0	0.0	0.0	0.13959123472397808	0.0017558645877229948
26	0.0	0.0	0.0	0.15627194830734653	0.0017558645877229948
27	0.0	0.0	0.0	0.18699957859249894	0.0017558645877229948
28	0.0	0.0	0.0	0.3046425059699396	0.0017558645877229948
29	0.0	0.0	0.0	0.5662663295406658	0.0017558645877229948
30	0.0	0.0	0.0	0.9657255232476472	0.0017558645877229948
31	0.0	0.0	0.0	1.9279393173198482	0.0017558645877229948
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCTT	25	1.2305204E-4	37.0	23
AGGTAGC	35	2.3799716E-5	31.714285	7
TAGAGAC	35	2.3799716E-5	31.714285	33
AAGGTAG	35	2.3799716E-5	31.714285	6
TCGCTTA	30	3.5905043E-4	30.833332	24
TAGGCTG	30	3.5905043E-4	30.833332	33
ATGCCCC	305	0.0	29.721312	37
GATACAA	25	0.0054872106	29.6	5
TCGGGTA	40	5.9195612E-5	27.75	25
CTAAGGT	40	5.9195612E-5	27.75	4
TAAGGTA	40	5.9195612E-5	27.75	5
ATATGCT	60	4.290814E-8	27.749998	35
ATTGACT	180	0.0	25.694445	31
TATTGAC	185	0.0	25.0	30
GTCGGGT	45	1.3186673E-4	24.666666	24
TGACTGT	190	0.0	24.342106	33
TATGCTG	70	1.9088839E-7	23.785713	36
TCCTAAG	55	1.893991E-5	23.545454	2
TTGACTG	205	0.0	23.463413	32
TCCTGAT	40	0.001926463	23.125	2
>>END_MODULE