FastQCFastQC Report
Fri 10 Feb 2017
ERR1631378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655763
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.20815447044130272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11250.1715558822318429No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11010.1678960234108969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10220.15584898812528306No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9070.13831216460825024No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT8400.1280950587331094No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT8050.12275776461922981No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC7950.12123282344383565No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7550.11513305874225903No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC7450.11360811756686487No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC7430.11330312933178603No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG7000.10674588227759114No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA7000.10674588227759114No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC6960.10613590580743348No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG6900.10522094110219699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG250.00549464429.59999817
ATATCGC250.00549464429.59999818
CGTGAAT451.3223653E-424.66666810
GCTTATT601.335944E-624.66666627
TCGTTTA1250.023.6830
TCTATCC400.001930307723.1253
CCGCTTA502.7003878E-422.19999925
GTCTAGA855.1721145E-821.7647061
CGGAGTC806.9507405E-720.812527
GGTATCA6850.020.2554761
GCGTTAT1650.020.1818181
TCATATA555.1398564E-420.1818182
ACAACGG851.2436394E-619.58823423
TAACGCC1551.8189894E-1219.0967754
AATAACG1551.8189894E-1219.0967752
GAATAAC1750.019.028571
ATAACGC1750.019.028573
CTAGAAC1205.160473E-918.53
CGTTATT1800.018.52
CATACCT609.2310505E-418.54
Produced by FastQC (version 0.11.5)