##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631378.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	655763
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72098761290283	34.0	31.0	34.0	31.0	34.0
2	32.892758511840405	34.0	31.0	34.0	31.0	34.0
3	33.01051141952199	34.0	33.0	34.0	31.0	34.0
4	36.37955785855561	37.0	37.0	37.0	35.0	37.0
5	36.32800874706258	37.0	37.0	37.0	35.0	37.0
6	36.357777123747454	37.0	37.0	37.0	35.0	37.0
7	36.36018805574575	37.0	37.0	37.0	35.0	37.0
8	36.35408981598535	37.0	37.0	37.0	35.0	37.0
9	38.17718901493375	39.0	38.0	39.0	37.0	39.0
10	38.121609483914156	39.0	38.0	39.0	37.0	39.0
11	38.181228584107366	39.0	38.0	39.0	37.0	39.0
12	37.965505830612585	39.0	38.0	39.0	37.0	39.0
13	38.10187369522221	39.0	38.0	39.0	37.0	39.0
14	39.58428426123462	41.0	39.0	41.0	37.0	41.0
15	39.56793231701087	41.0	39.0	41.0	37.0	41.0
16	39.54818737867187	41.0	39.0	41.0	37.0	41.0
17	39.54511004737992	41.0	39.0	41.0	37.0	41.0
18	39.5225027944547	41.0	39.0	41.0	37.0	41.0
19	39.5195657577509	41.0	39.0	41.0	37.0	41.0
20	39.48113571518979	41.0	39.0	41.0	37.0	41.0
21	39.45322014203302	41.0	39.0	41.0	37.0	41.0
22	39.39545384536792	41.0	39.0	41.0	37.0	41.0
23	39.33644472164486	41.0	39.0	41.0	36.0	41.0
24	39.292759731793346	41.0	39.0	41.0	36.0	41.0
25	39.292581313675825	41.0	39.0	41.0	36.0	41.0
26	39.21302208267316	40.0	39.0	41.0	36.0	41.0
27	39.142952560604975	40.0	39.0	41.0	36.0	41.0
28	39.056971802312724	40.0	39.0	41.0	35.0	41.0
29	38.99072378283008	40.0	39.0	41.0	35.0	41.0
30	38.9384777732199	40.0	39.0	41.0	35.0	41.0
31	38.90534537630211	40.0	38.0	41.0	35.0	41.0
32	38.82400043918306	40.0	38.0	41.0	35.0	41.0
33	38.72929854230873	40.0	38.0	41.0	35.0	41.0
34	38.683722625399724	40.0	38.0	41.0	35.0	41.0
35	38.58441845605806	40.0	38.0	41.0	35.0	41.0
36	38.533186532329516	40.0	38.0	41.0	35.0	41.0
37	38.47068224343246	40.0	38.0	41.0	35.0	41.0
38	38.378441601615215	40.0	38.0	41.0	34.0	41.0
39	38.30330012519767	40.0	38.0	41.0	34.0	41.0
40	38.20684759585399	40.0	38.0	41.0	34.0	41.0
41	38.10169985192821	40.0	37.0	41.0	34.0	41.0
42	37.97718992989845	40.0	37.0	41.0	34.0	41.0
43	37.030010842331755	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	1.0
14	1.0
15	1.0
16	3.0
17	11.0
18	12.0
19	26.0
20	59.0
21	103.0
22	185.0
23	321.0
24	540.0
25	781.0
26	1221.0
27	1697.0
28	2365.0
29	3250.0
30	4443.0
31	6044.0
32	7772.0
33	10598.0
34	15371.0
35	22030.0
36	35376.0
37	67408.0
38	159673.0
39	316467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5852586986457	17.86056852856901	11.869379638680439	26.68479313410485
2	19.56865513912801	19.646884621425727	33.391179435253285	27.393280804192976
3	20.17344680928933	21.351921349633937	28.65105228565808	29.82357955541865
4	14.650109872011688	14.87625864832264	33.33155423529537	37.1420772443703
5	16.313058223779016	34.76408397546065	32.32311063600722	16.59974716475312
6	35.68240355128301	33.693575270333945	15.362409895038297	15.261611283344745
7	31.054817060431894	29.333920943999587	20.028729891744426	19.582532103824096
8	28.852497014927646	31.217070801493836	19.327409445180653	20.603022738397865
9	27.74554221570903	13.69397175503955	18.84247815140531	39.71800787784611
10	18.810454386722032	24.613160547331887	30.348921790341937	26.227463275604144
11	38.554477761020365	20.47370772672444	19.97047713884437	21.00133737341082
12	23.874479041970957	24.537370970914797	26.708582216441002	24.879567770673248
13	31.13152160155422	17.63045490520203	24.36185024162693	26.87617325161682
14	24.070586477126646	19.425158174523418	23.128325324850593	33.37593002349934
15	26.90529962806685	26.177597699168754	20.518083514928414	26.399019157835983
16	27.12321372203067	24.286365653444918	22.401843348892818	26.18857727563159
17	25.618401770151717	25.086349794056694	23.263435112990514	26.031813322801074
18	26.27244904027827	22.696919466331586	24.837784382467447	26.192847110922695
19	26.132764428612166	23.847030100813864	24.09773043004866	25.92247504052531
20	26.325974475534608	22.9697314426096	24.129449206496858	26.574844875358934
21	28.278204168274208	23.29362894826332	23.727932195015576	24.700234688446894
22	27.576426239357815	22.874880101500082	23.285546760033732	26.26314689910837
23	26.010921628698174	22.71460878396616	24.226892947604547	27.047576639731126
24	25.56258892313229	23.883018712553163	23.953013512503755	26.601378851810793
25	26.31118254613328	22.97796612495673	24.099865347694212	26.610985981215773
26	26.159450899181564	24.230247818190414	23.981072430131007	25.629228852497015
27	27.074110616182978	22.84239885446419	23.742266642064283	26.341223887288546
28	25.47124494672618	23.295306383556255	24.75589504134878	26.47755362836878
29	25.315090970365816	23.46899718343365	24.74293304135793	26.4729788048426
30	24.64152445319422	24.171385088820198	25.273307582160022	25.913782875825564
31	26.300202969670444	23.679134077402963	23.549056595141842	26.47160635778475
32	25.456300523207315	23.08776188958511	24.083853465352572	27.372084121855
33	24.991650947064716	22.86313805444955	24.799813347200132	27.3453976512856
34	26.21999106384471	22.61213273697967	24.630087394378762	26.537788804796854
35	26.14969127565904	22.663980736943074	25.386153229139186	25.8001747582587
36	24.721888853137493	23.345019465874103	25.252873370409734	26.680218310578667
37	25.804292099432264	22.190181513748108	25.64920558189468	26.356320804924948
38	24.671260806114404	21.70143786703428	26.791539016382444	26.835762310468876
39	25.32271567624279	21.350091420223468	26.516439628341338	26.81075327519241
40	24.788528782502215	22.25682144311283	26.7929114634403	26.161738310944653
41	23.713597747966872	22.099142525577076	28.057545180194676	26.12971454626138
42	24.53843842973757	22.21793544314028	27.695829133391182	25.547796993730966
43	22.809765113310753	21.48520120836339	27.88675176855053	27.818281909775333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	91.5
2	79.0
3	161.0
4	243.0
5	243.0
6	294.0
7	345.0
8	308.5
9	272.0
10	438.0
11	604.0
12	604.0
13	968.5
14	1333.0
15	2013.0
16	2693.0
17	2476.5
18	2260.0
19	2260.0
20	2581.0
21	2902.0
22	2523.0
23	2144.0
24	2499.0
25	2854.0
26	2854.0
27	3437.5
28	4021.0
29	5110.0
30	6199.0
31	7691.0
32	9183.0
33	9183.0
34	11713.5
35	14244.0
36	17761.0
37	21278.0
38	24901.5
39	28525.0
40	28525.0
41	30895.5
42	33266.0
43	35333.5
44	37401.0
45	40495.5
46	43590.0
47	43590.0
48	47087.5
49	50585.0
50	51831.0
51	53077.0
52	54002.0
53	54927.0
54	54927.0
55	53445.0
56	51963.0
57	49734.0
58	47505.0
59	45778.5
60	44052.0
61	44052.0
62	40832.5
63	37613.0
64	34720.0
65	31827.0
66	28638.5
67	25450.0
68	25450.0
69	20977.0
70	16504.0
71	14210.5
72	11917.0
73	8936.0
74	5955.0
75	5955.0
76	4597.5
77	3240.0
78	2709.5
79	2179.0
80	2013.5
81	1848.0
82	1848.0
83	1515.0
84	1182.0
85	1108.0
86	1034.0
87	928.0
88	822.0
89	822.0
90	638.0
91	454.0
92	259.5
93	65.0
94	41.0
95	17.0
96	17.0
97	11.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	655763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.21719694422549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.49252648295452	42.931950396539406
2	6.887993984816287	6.9179150097232025
3	2.4542026050207317	3.69729526672072
4	1.242861781574644	2.496521394391395
5	0.7460704910430288	1.8732784391491404
6	0.5375368926495344	1.6196157601781205
7	0.3646770250671076	1.2819140592180438
8	0.2633430545201072	1.057948002618411
9	0.21334105165424672	0.9642050646488545
>10	1.4767948451963757	14.788557112136388
>50	0.1770582007401405	6.248552889193843
>100	0.13537954495695198	13.127292303392348
>500	0.0069971450202468985	2.290214239065181
>1k	0.0012168947861298954	0.7047400630249937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1365	0.20815447044130272	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1125	0.1715558822318429	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1101	0.1678960234108969	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1022	0.15584898812528306	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	907	0.13831216460825024	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	840	0.1280950587331094	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	805	0.12275776461922981	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	795	0.12123282344383565	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	755	0.11513305874225903	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	745	0.11360811756686487	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	743	0.11330312933178603	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	700	0.10674588227759114	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	700	0.10674588227759114	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	696	0.10613590580743348	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	690	0.10522094110219699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	1.5249411753941592E-4	0.0	0.0	0.0
10	0.0	1.5249411753941592E-4	0.0	1.5249411753941592E-4	0.0
11	0.0	1.5249411753941592E-4	0.0	3.0498823507883185E-4	0.0
12	0.0	1.5249411753941592E-4	0.0	3.0498823507883185E-4	0.0
13	0.0	1.5249411753941592E-4	0.0	3.0498823507883185E-4	0.0
14	0.0	1.5249411753941592E-4	0.0	6.099764701576637E-4	0.0
15	0.0	1.5249411753941592E-4	0.0	0.0010674588227759115	0.0
16	0.0	1.5249411753941592E-4	0.0	0.0015249411753941592	0.0
17	0.0	1.5249411753941592E-4	0.0	0.002134917645551823	0.0
18	0.0	3.0498823507883185E-4	0.0	0.0028973882332489023	0.0
19	0.0	3.0498823507883185E-4	0.0	0.0030498823507883184	0.0
20	0.0	3.0498823507883185E-4	0.0	0.00335487058586715	0.0
21	0.0	3.0498823507883185E-4	0.0	0.00411734117356423	0.0
22	0.0	3.0498823507883185E-4	0.0	0.006099764701576637	0.0
23	0.0	3.0498823507883185E-4	0.0	0.00823468234712846	0.0
24	0.0	3.0498823507883185E-4	0.0	0.012352023520692689	0.0
25	0.0	3.0498823507883185E-4	0.0	0.015249411753941592	0.0
26	0.0	3.0498823507883185E-4	0.0	0.021501670573057644	0.0
27	0.0	3.0498823507883185E-4	0.0	0.050170564670467835	0.0
28	0.0	3.0498823507883185E-4	0.0	0.1619487528268597	0.0
29	0.0	3.0498823507883185E-4	0.0	0.328167340944823	0.0
30	0.0	3.0498823507883185E-4	0.0	0.5491313172594368	0.0
31	0.0	3.0498823507883185E-4	0.0	1.105429858043226	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	25	0.005494644	29.599998	17
ATATCGC	25	0.005494644	29.599998	18
CGTGAAT	45	1.3223653E-4	24.666668	10
GCTTATT	60	1.335944E-6	24.666666	27
TCGTTTA	125	0.0	23.68	30
TCTATCC	40	0.0019303077	23.125	3
CCGCTTA	50	2.7003878E-4	22.199999	25
GTCTAGA	85	5.1721145E-8	21.764706	1
CGGAGTC	80	6.9507405E-7	20.8125	27
GGTATCA	685	0.0	20.255476	1
GCGTTAT	165	0.0	20.181818	1
TCATATA	55	5.1398564E-4	20.181818	2
ACAACGG	85	1.2436394E-6	19.588234	23
TAACGCC	155	1.8189894E-12	19.096775	4
AATAACG	155	1.8189894E-12	19.096775	2
GAATAAC	175	0.0	19.02857	1
ATAACGC	175	0.0	19.02857	3
CTAGAAC	120	5.160473E-9	18.5	3
CGTTATT	180	0.0	18.5	2
CATACCT	60	9.2310505E-4	18.5	4
>>END_MODULE