FastQCFastQC Report
Fri 10 Feb 2017
ERR1631377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4465022
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA131610.2947577861878396No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC113800.2548699648064444No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC97270.21784887062146616No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC92160.20640435814202035No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC90530.20275376022783317No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT73810.16530713622463672No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC71050.1591257557073627No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT70120.157042899228716No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC63770.1428212447777413No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC62460.13988732866265832No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC60480.13545286003070087No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG60300.13504972651870473No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC58260.13048088004941522No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC58100.13012253914986308No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT56810.12723341564722412No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC55280.12380678079525699No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG54740.12259738025926861No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT54610.1223062282783825No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG54500.12205986890994043No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA54150.12127599819217016No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG54000.12094005359884005No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG53820.12053692008684393No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG51690.11576650686155633No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG51670.11572171424911232No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG50170.1123622683158112No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA49410.11066014904293864No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT48550.10873406670784602No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC47240.10580015059276303No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC47060.1053970170807669No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG46920.10508346879365879No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG46210.10349333105189627No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC45580.1020823637599098No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT45520.10194798592257776No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA44930.10062660385547932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.023.2011741
CTTATAC45250.019.78784637
AGTCGGT25000.019.16611
CTCTATG37100.018.9986521
GCAGTCG25600.018.9335949
GCCGTAT3800.018.58
CCGTATG3750.018.2533349
TCTATGG40150.017.7397252
AAGACGG18500.017.45
GCCTTAT19200.017.15104326
TCGGTGA27750.017.06666813
CAGTCGG28650.016.98254810
GGCAGTC28500.016.9421048
CAAGACG18850.016.8806364
CGGTGAT28250.016.69911614
TAGGCCG4550.016.670335
ATACACA9550.016.65968537
CGCCTTA20200.016.57673325
TAGAACT11050.016.574664
TTAGAAC7150.016.559443
Produced by FastQC (version 0.11.5)