##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631377.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4465022
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81919327609136	34.0	31.0	34.0	31.0	34.0
2	32.99689564799457	34.0	33.0	34.0	31.0	34.0
3	33.113700671575636	34.0	33.0	34.0	31.0	34.0
4	36.44542132155228	37.0	37.0	37.0	35.0	37.0
5	36.42507741283246	37.0	37.0	37.0	35.0	37.0
6	36.469030387756206	37.0	37.0	37.0	35.0	37.0
7	36.452327446538895	37.0	37.0	37.0	35.0	37.0
8	36.44058103185158	37.0	37.0	37.0	35.0	37.0
9	38.2906379856583	39.0	39.0	39.0	37.0	39.0
10	38.24826954939976	39.0	39.0	39.0	37.0	39.0
11	38.30820609618497	39.0	39.0	39.0	37.0	39.0
12	38.08441951685792	39.0	39.0	39.0	37.0	39.0
13	38.214940486295475	39.0	39.0	39.0	37.0	39.0
14	39.73269336634848	41.0	40.0	41.0	38.0	41.0
15	39.73406469217845	41.0	40.0	41.0	38.0	41.0
16	39.718611688811386	41.0	40.0	41.0	38.0	41.0
17	39.70724757907128	41.0	40.0	41.0	38.0	41.0
18	39.68846088552307	41.0	40.0	41.0	37.0	41.0
19	39.690499173352336	41.0	40.0	41.0	37.0	41.0
20	39.677936189340166	41.0	40.0	41.0	37.0	41.0
21	39.642699632834955	41.0	40.0	41.0	37.0	41.0
22	39.58876977537849	41.0	40.0	41.0	37.0	41.0
23	39.51280889545449	41.0	39.0	41.0	37.0	41.0
24	39.47741175743367	41.0	39.0	41.0	37.0	41.0
25	39.46017936753727	41.0	39.0	41.0	37.0	41.0
26	39.404420627714714	41.0	39.0	41.0	36.0	41.0
27	39.32661048478597	41.0	39.0	41.0	36.0	41.0
28	39.20084178756566	41.0	39.0	41.0	36.0	41.0
29	39.12389703790933	41.0	39.0	41.0	35.0	41.0
30	39.068154647390315	40.0	39.0	41.0	35.0	41.0
31	39.03657831921097	40.0	39.0	41.0	35.0	41.0
32	38.954708845779486	40.0	39.0	41.0	35.0	41.0
33	38.84050627298141	40.0	38.0	41.0	35.0	41.0
34	38.79778867830886	40.0	38.0	41.0	35.0	41.0
35	38.685472770346934	40.0	38.0	41.0	35.0	41.0
36	38.62460341740757	40.0	38.0	41.0	35.0	41.0
37	38.56135692052581	40.0	38.0	41.0	35.0	41.0
38	38.45941968483022	40.0	38.0	41.0	35.0	41.0
39	38.371908805824475	40.0	38.0	41.0	34.0	41.0
40	38.264376972834626	40.0	37.0	41.0	34.0	41.0
41	38.17567125089193	40.0	37.0	41.0	34.0	41.0
42	38.034716514274734	40.0	37.0	41.0	34.0	41.0
43	37.07904216373402	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	3.0
12	5.0
13	5.0
14	9.0
15	3.0
16	26.0
17	46.0
18	73.0
19	190.0
20	391.0
21	706.0
22	1194.0
23	2048.0
24	3243.0
25	4906.0
26	7174.0
27	10024.0
28	13940.0
29	19181.0
30	25984.0
31	34235.0
32	46079.0
33	62732.0
34	95641.0
35	140687.0
36	234319.0
37	434263.0
38	1001780.0
39	2326133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94647999494739	17.169030746097107	11.587714461429306	27.29677479752619
2	20.6130227353863	19.175090290708535	31.816886904476622	28.39500006942855
3	20.853178327004883	20.32131084684465	28.04064571238395	30.784865113766518
4	14.619233679027785	14.579032309359283	32.87130500140873	37.9304290102042
5	16.97416048565942	34.24090631580315	31.6452864062036	17.139646792333835
6	36.59390704009969	32.90176397787066	15.198805291440893	15.30552369058876
7	31.698365651949757	28.576589320276586	19.308527483179255	20.416517544594406
8	29.962629523437954	29.558532970274275	18.8105232180267	21.668314288261065
9	27.910254417559422	13.450191286851442	18.213079353248425	40.42647494234071
10	19.3837790720852	23.370231994377633	29.373382706736944	27.87260622680023
11	39.925693535216624	20.004246339659694	18.93408363945351	21.13597648567017
12	24.984759313615925	23.55119414865145	25.474118604566787	25.98992793316584
13	31.876841816232933	16.598328070947915	23.757083391750367	27.767746721068786
14	24.67575747667089	18.997039656243576	21.5513159845573	34.77588688252824
15	28.051082391083405	25.199181549385425	19.375447646170613	27.37428841336056
16	28.496634507064016	23.484430759803647	21.166121913844993	26.85281281928734
17	27.215543394858972	23.605975513670483	21.7838568320604	27.394624259410143
18	27.93880970799248	20.868027078030075	23.693836222979417	27.49932699099803
19	27.151982677800916	23.22295388466171	22.307012149100274	27.3180512884371
20	27.667030531988416	21.218484477792046	22.438657637073234	28.6758273531463
21	29.992842140531444	22.073866601329176	22.29679943346304	25.636491824676337
22	29.710155963397266	21.269682433815557	21.742580439693242	27.277581163093934
23	27.35639376468918	21.2976106276744	22.60586845932674	28.740127148309686
24	26.628379434636607	22.815811433851838	22.694737002415664	27.86107212909589
25	27.280582268127684	21.64560443375195	22.336620065925768	28.737193232194603
26	27.41923779994813	23.30673846623824	22.520493740008448	26.75352999380518
27	28.762097924713476	21.267420406887133	22.496798448025565	27.47368322037383
28	26.818188129868116	21.890642420126934	23.425528474439766	27.86564097556518
29	25.610870450358362	22.406698108094428	24.126779218557044	27.85565222299017
30	25.17120856291414	23.82962502760345	24.294841100446984	26.704325309035433
31	27.436012633308415	23.06631411894499	21.73496569557776	27.762707552168838
32	26.59182866288229	22.182197534525024	22.74176924548188	28.484204557110804
33	25.74168727500111	21.705021834158934	23.64646803531987	28.906822855520087
34	27.584768899234984	21.864438741847184	23.38293070000551	27.16786165891232
35	27.48228340196308	21.55888593606034	24.3880769232492	26.570753738727383
36	25.27078254037718	23.206044673464095	24.04062062852098	27.482552157637745
37	27.044010085504617	21.3460538380326	24.62621684730781	26.983719229154975
38	25.29759091892492	20.543258241504745	25.93402675283571	28.22512408673462
39	26.26452904375387	20.308276196623442	25.595013865553184	27.832180894069502
40	25.126438346776343	20.98500298542762	26.261102408901905	27.627456258894135
41	23.92324158761144	21.282403535749655	27.740938342521044	27.053416534117865
42	25.41026673552784	21.066704710525503	27.532047994388382	25.990980559558274
43	23.083088952305275	20.069800327971507	27.861116921708334	28.98599379801488
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	47.0
2	73.0
3	247.5
4	422.0
5	422.0
6	571.5
7	721.0
8	731.5
9	742.0
10	1044.5
11	1347.0
12	1347.0
13	2391.5
14	3436.0
15	5730.0
16	8024.0
17	7726.5
18	7429.0
19	7429.0
20	9235.0
21	11041.0
22	10027.5
23	9014.0
24	10695.0
25	12376.0
26	12376.0
27	15283.0
28	18190.0
29	23560.5
30	28931.0
31	36394.5
32	43858.0
33	43858.0
34	57606.5
35	71355.0
36	92598.0
37	113841.0
38	134031.5
39	154222.0
40	154222.0
41	172095.5
42	189969.0
43	207984.0
44	225999.0
45	249803.0
46	273607.0
47	273607.0
48	303917.5
49	334228.0
50	340849.0
51	347470.0
52	362850.0
53	378230.0
54	378230.0
55	366812.5
56	355395.0
57	350329.0
58	345263.0
59	342652.0
60	340041.0
61	340041.0
62	329182.0
63	318323.0
64	296931.0
65	275539.0
66	245546.5
67	215554.0
68	215554.0
69	178300.0
70	141046.0
71	119918.0
72	98790.0
73	72705.0
74	46620.0
75	46620.0
76	36530.0
77	26440.0
78	23184.0
79	19928.0
80	18914.0
81	17900.0
82	17900.0
83	14617.0
84	11334.0
85	9652.5
86	7971.0
87	6830.0
88	5689.0
89	5689.0
90	4552.0
91	3415.0
92	2104.0
93	793.0
94	536.0
95	279.0
96	279.0
97	204.0
98	129.0
99	78.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4465022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.632081012532765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.49365914385677	30.95178566803091
2	7.883005122186165	5.775417645162687
3	2.4474501606401136	2.6896557767611484
4	1.2338364243768103	1.807919834159403
5	0.7438976870383601	1.3625260168312474
6	0.486149093271032	1.0685191781324275
7	0.35017424016941484	0.8979327794071676
8	0.258395447498547	0.7572450372829145
9	0.21633024638167933	0.7132164399823365
>10	1.4883494577870051	10.757381716694258
>50	0.18160880896323475	4.679585024753544
>100	0.16979379946616238	13.153313126627546
>500	0.02373651885768	6.0414206191280435
>1k	0.022080482656259566	15.535367257659475
>5k	0.0014106975030388057	3.2573225811375734
>10k+	1.2266934809033092E-4	0.551391298249334
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	13161	0.2947577861878396	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	11380	0.2548699648064444	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	9727	0.21784887062146616	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	9216	0.20640435814202035	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	9053	0.20275376022783317	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7381	0.16530713622463672	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	7105	0.1591257557073627	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	7012	0.157042899228716	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	6377	0.1428212447777413	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	6246	0.13988732866265832	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	6048	0.13545286003070087	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	6030	0.13504972651870473	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	5826	0.13048088004941522	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	5810	0.13012253914986308	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	5681	0.12723341564722412	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	5528	0.12380678079525699	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	5474	0.12259738025926861	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	5461	0.1223062282783825	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	5450	0.12205986890994043	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	5415	0.12127599819217016	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	5400	0.12094005359884005	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	5382	0.12053692008684393	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	5169	0.11576650686155633	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	5167	0.11572171424911232	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	5017	0.1123622683158112	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	4941	0.11066014904293864	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	4855	0.10873406670784602	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	4724	0.10580015059276303	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	4706	0.1053970170807669	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	4692	0.10508346879365879	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	4621	0.10349333105189627	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	4558	0.1020823637599098	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT	4552	0.10194798592257776	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	4493	0.10062660385547932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2396306222007418E-5	0.0	2.2396306222007418E-5	0.0
3	0.0	2.2396306222007418E-5	0.0	2.2396306222007418E-5	0.0
4	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
5	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
6	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
7	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
8	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
9	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
10	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
11	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
12	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
13	0.0	4.4792612444014835E-5	0.0	2.2396306222007418E-5	0.0
14	0.0	4.4792612444014835E-5	0.0	6.718891866602225E-5	0.0
15	0.0	4.4792612444014835E-5	0.0	1.7917044977605934E-4	0.0
16	0.0	4.4792612444014835E-5	0.0	5.37511349328178E-4	0.0
17	0.0	4.4792612444014835E-5	0.0	7.614744115482521E-4	0.0
18	0.0	1.567741435540519E-4	0.0	9.630411675463189E-4	2.2396306222007418E-5
19	0.0	1.567741435540519E-4	0.0	0.0012317968422104078	2.2396306222007418E-5
20	0.0	1.567741435540519E-4	0.0	0.001612534047984534	6.718891866602225E-5
21	0.0	1.567741435540519E-4	0.0	0.002150045397312712	6.718891866602225E-5
22	0.0	1.567741435540519E-4	0.0	0.003225068095969068	6.718891866602225E-5
23	0.0	1.7917044977605934E-4	0.0	0.004658431694177543	6.718891866602225E-5
24	0.0	1.7917044977605934E-4	0.0	0.007816310871480589	6.718891866602225E-5
25	0.0	1.7917044977605934E-4	0.0	0.009204881857245049	6.718891866602225E-5
26	0.0	1.7917044977605934E-4	0.0	0.01368414310164653	6.718891866602225E-5
27	0.0	1.7917044977605934E-4	0.0	0.031153261954812318	6.718891866602225E-5
28	0.0	2.4635936844208156E-4	0.0	0.11480346569401002	6.718891866602225E-5
29	0.0	2.9115198088609643E-4	0.0	0.2463369721358596	8.958522488802967E-5
30	0.0	2.9115198088609643E-4	0.0	0.4255074219119189	8.958522488802967E-5
31	0.0	2.9115198088609643E-4	0.0	0.9585619063019174	8.958522488802967E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4250	0.0	23.201174	1
CTTATAC	4525	0.0	19.787846	37
AGTCGGT	2500	0.0	19.166	11
CTCTATG	3710	0.0	18.998652	1
GCAGTCG	2560	0.0	18.933594	9
GCCGTAT	380	0.0	18.5	8
CCGTATG	375	0.0	18.253334	9
TCTATGG	4015	0.0	17.739725	2
AAGACGG	1850	0.0	17.4	5
GCCTTAT	1920	0.0	17.151043	26
TCGGTGA	2775	0.0	17.066668	13
CAGTCGG	2865	0.0	16.982548	10
GGCAGTC	2850	0.0	16.942104	8
CAAGACG	1885	0.0	16.880636	4
CGGTGAT	2825	0.0	16.699116	14
TAGGCCG	455	0.0	16.67033	5
ATACACA	955	0.0	16.659685	37
CGCCTTA	2020	0.0	16.576733	25
TAGAACT	1105	0.0	16.57466	4
TTAGAAC	715	0.0	16.55944	3
>>END_MODULE