FastQCFastQC Report
Fri 10 Feb 2017
ERR1631376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7153891
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA130480.18239025447829718No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC121650.17004732110120213No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC89590.12523254827338018No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT84600.11825732318258694No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC83100.11616056213325028No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA81030.1132670318851657No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC80240.11216273773251507No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT73640.10293698911543384No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT73260.10240580964960186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC17350.021.219028
TCTAGAC22450.021.0957683
CTAGACA23000.020.9934794
GGTATCA51050.020.8011761
TAGAACT25150.020.1550714
GTCTAGA21500.020.1348821
ACATGTA19200.019.2708348
ACGGAGT17800.019.2275326
GAATCTA8100.019.18518413
CTAGAAC24000.018.8854163
GTTCTAG25950.018.6782281
CTCTAGT23500.018.34255427
AACGGAG18650.018.15281725
AGTGCGC11550.017.93939429
ACAACGG19100.017.82199123
AATCTAC8750.017.7614
CTTATAC63800.017.6590937
GGCACTA10250.017.14634112
GACATGT21700.017.1359447
TCTAGTT25950.017.03853628
Produced by FastQC (version 0.11.5)