##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631376.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7153891
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.892111299990454	34.0	33.0	34.0	31.0	34.0
2	33.059641669128034	34.0	33.0	34.0	31.0	34.0
3	33.1778460141481	34.0	33.0	34.0	31.0	34.0
4	36.484581327839635	37.0	37.0	37.0	35.0	37.0
5	36.46780122872993	37.0	37.0	37.0	35.0	37.0
6	36.51396282107178	37.0	37.0	37.0	35.0	37.0
7	36.50022833727827	37.0	37.0	37.0	35.0	37.0
8	36.48307054161155	37.0	37.0	37.0	35.0	37.0
9	38.34156545018648	39.0	39.0	39.0	37.0	39.0
10	38.301209928974316	39.0	39.0	39.0	37.0	39.0
11	38.3620610098756	39.0	39.0	39.0	37.0	39.0
12	38.144908693744426	39.0	39.0	39.0	37.0	39.0
13	38.26964696554644	39.0	39.0	39.0	37.0	39.0
14	39.819433228714274	41.0	40.0	41.0	38.0	41.0
15	39.831383788207006	41.0	40.0	41.0	38.0	41.0
16	39.83027012852167	41.0	40.0	41.0	38.0	41.0
17	39.81961215232382	41.0	40.0	41.0	38.0	41.0
18	39.80633755812047	41.0	40.0	41.0	38.0	41.0
19	39.81404007972724	41.0	40.0	41.0	38.0	41.0
20	39.78760313792871	41.0	40.0	41.0	38.0	41.0
21	39.7563953658226	41.0	40.0	41.0	38.0	41.0
22	39.71863647349394	41.0	40.0	41.0	38.0	41.0
23	39.669859521203215	41.0	40.0	41.0	37.0	41.0
24	39.64186678270608	41.0	40.0	41.0	37.0	41.0
25	39.61892667920157	41.0	40.0	41.0	37.0	41.0
26	39.57148214866567	41.0	40.0	41.0	37.0	41.0
27	39.50388019610587	41.0	39.0	41.0	37.0	41.0
28	39.43078151456319	41.0	39.0	41.0	37.0	41.0
29	39.376794111064875	41.0	39.0	41.0	36.0	41.0
30	39.32866352031363	41.0	39.0	41.0	36.0	41.0
31	39.29310021637176	41.0	39.0	41.0	36.0	41.0
32	39.21563230415448	41.0	39.0	41.0	36.0	41.0
33	39.13558984893675	41.0	39.0	41.0	35.0	41.0
34	39.098853756647955	41.0	39.0	41.0	35.0	41.0
35	39.01448190921556	40.0	39.0	41.0	35.0	41.0
36	38.96574269862373	40.0	39.0	41.0	35.0	41.0
37	38.909774275286	40.0	39.0	41.0	35.0	41.0
38	38.81764357326663	40.0	38.0	41.0	35.0	41.0
39	38.749760514942146	40.0	38.0	41.0	35.0	41.0
40	38.66381455909798	40.0	38.0	41.0	35.0	41.0
41	38.58166583751416	40.0	38.0	41.0	35.0	41.0
42	38.469364154416105	40.0	38.0	41.0	35.0	41.0
43	37.55036734554664	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	3.0
10	6.0
11	2.0
12	1.0
13	3.0
14	9.0
15	7.0
16	25.0
17	52.0
18	92.0
19	218.0
20	377.0
21	783.0
22	1427.0
23	2388.0
24	3829.0
25	5945.0
26	8839.0
27	13130.0
28	18330.0
29	25569.0
30	35542.0
31	47411.0
32	63085.0
33	85496.0
34	126419.0
35	188097.0
36	314706.0
37	603420.0
38	1513354.0
39	4095325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58665920406112	16.86329858813896	11.154433300703072	27.395608907096854
2	20.034188387829786	18.94436188641957	33.323082501536575	27.698367224214067
3	20.40825055903144	20.18502937771906	27.961119340509942	31.445600722739552
4	14.847877889109576	14.224804375688699	32.74159474892755	38.185722986274186
5	16.698213601521186	34.04861773823504	32.22760872370015	17.025559936543626
6	37.085342787582306	32.82571680222693	14.887995917186887	15.200944493003876
7	32.29302766843946	28.174597572146403	19.432837318880033	20.09953744053411
8	28.803695778982373	31.51668092231207	18.775852190087882	20.903771108617676
9	27.780700041418022	13.54035167715024	18.129364844949414	40.54958343648233
10	18.22666853604563	24.582622240120795	30.53904511544836	26.651664108385216
11	39.06149814136111	20.220478617859847	19.5995158438953	21.118507396883736
12	24.647915379197137	23.664716166349194	26.422474147285723	25.264894307167946
13	31.766125595148154	17.287375499570793	23.900853395725488	27.04564550955557
14	24.510186135069713	18.679862469249255	22.299598917568076	34.510352478112964
15	27.13485570300135	25.58571272612345	20.05821726945518	27.221214301420027
16	27.600937727454895	23.762425231248283	21.697087640837694	26.939549400459136
17	26.45234600303527	24.31023900140497	22.606858281737868	26.63055671382189
18	27.234787334612733	22.182907175968992	23.856793456875426	26.72551203254285
19	26.72686793802142	23.1908761260131	23.130070055582337	26.95218588038314
20	26.83802423045025	22.570067114525507	23.456955103173925	27.134953551850316
21	28.271733522358673	22.685710475599922	23.4690324468181	25.573523555223304
22	28.015984587967584	22.355428675108413	22.67982556625478	26.948761170669222
23	26.596099940577794	22.273179728346435	23.63453678564574	27.496183545430032
24	26.354077801856363	23.1978373726969	23.06894248179068	27.37914234365606
25	27.175938241161347	22.68189437049013	23.16913970313498	26.97302768521354
26	27.251827012740335	23.320274239571166	22.906080061885202	26.521818685803293
27	27.55740896807066	22.219824148844314	23.15627956869905	27.066487314385974
28	26.2189904766511	22.715638244977455	23.90286628633285	27.1625049920386
29	26.05575063975674	22.849565362402082	23.550722257300258	27.54396174054092
30	25.19413561095633	23.457416390604777	24.824965882203127	26.523482116235762
31	27.301506271202626	22.895302710091613	22.891067252771954	26.91212376593381
32	26.33735962708965	22.582214350204666	23.332030079854444	27.74839594285124
33	26.1801165267964	22.331958929762838	23.636074410415254	27.851850133025508
34	27.153698595631386	22.089727114936473	23.685292381446686	27.071281907985455
35	26.364212146928157	22.340471779623144	24.606832841037136	26.688483232411564
36	25.67583151602394	22.5511263730465	24.70907090980279	27.063971201126773
37	26.938822523295364	21.701029551610446	24.73066754861096	26.629480376483233
38	25.526332453206237	21.10856874950988	25.859997028190673	27.50510176909321
39	26.496322071443355	21.19252306192532	25.605799696976096	26.705355169655228
40	25.57432032442205	21.967234334434227	25.499340149297772	26.95910519184595
41	25.13699188315841	21.686995230986884	26.68226284129853	26.493750044556176
42	25.737112852292547	21.56376159491387	26.411151637619305	26.287973915174273
43	23.91800490110906	21.185505901613542	26.48346193700743	28.413027260269967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	128.0
1	162.5
2	197.0
3	546.5
4	896.0
5	896.0
6	1120.5
7	1345.0
8	1293.5
9	1242.0
10	1799.5
11	2357.0
12	2357.0
13	3992.0
14	5627.0
15	8658.5
16	11690.0
17	11252.0
18	10814.0
19	10814.0
20	12659.5
21	14505.0
22	13565.0
23	12625.0
24	15739.5
25	18854.0
26	18854.0
27	23813.0
28	28772.0
29	38271.0
30	47770.0
31	62048.0
32	76326.0
33	76326.0
34	101529.0
35	126732.0
36	160806.5
37	194881.0
38	233912.0
39	272943.0
40	272943.0
41	301508.5
42	330074.0
43	355204.5
44	380335.0
45	425982.5
46	471630.0
47	471630.0
48	510036.5
49	548443.0
50	566149.0
51	583855.0
52	604454.5
53	625054.0
54	625054.0
55	616546.0
56	608038.0
57	584614.5
58	561191.0
59	543397.0
60	525603.0
61	525603.0
62	497762.5
63	469922.0
64	436078.5
65	402235.0
66	360385.5
67	318536.0
68	318536.0
69	259389.0
70	200242.0
71	170514.5
72	140787.0
73	102623.0
74	64459.0
75	64459.0
76	48028.0
77	31597.0
78	25396.5
79	19196.0
80	17400.5
81	15605.0
82	15605.0
83	12747.5
84	9890.0
85	9268.5
86	8647.0
87	7556.5
88	6466.0
89	6466.0
90	4957.0
91	3448.0
92	2001.0
93	554.0
94	410.5
95	267.0
96	267.0
97	184.0
98	101.0
99	56.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7153891.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.144744678806482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8276363827937	22.748731889851385
2	8.953653957301334	5.0399660914126265
3	3.031510931776907	2.559633034976154
4	1.5165746341716806	1.707344234604652
5	0.9714535728297231	1.367065638730345
6	0.6557371610094712	1.107333298381019
7	0.5089230331703234	1.002645618081976
8	0.38122429553317594	0.8583568370511283
9	0.2942356488248345	0.745306849042014
>10	2.217155501262752	12.422399228698065
>50	0.27965853178546257	5.554415102213515
>100	0.286455463659586	17.299455601247953
>500	0.04287602997395676	8.341049720617924
>1k	0.031309468954028176	16.2159613675516
>5k	0.0014956752685044237	2.6765517262883787
>10k+	9.971168456696157E-5	0.35378376125121963
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	13048	0.18239025447829718	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	12165	0.17004732110120213	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	8959	0.12523254827338018	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	8460	0.11825732318258694	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	8310	0.11616056213325028	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	8103	0.1132670318851657	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	8024	0.11216273773251507	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	7364	0.10293698911543384	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7326	0.10240580964960186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	1.3978406995577652E-5	0.0	1.3978406995577652E-5	0.0
10	0.0	1.3978406995577652E-5	0.0	5.591362798231061E-5	0.0
11	0.0	2.7956813991155304E-5	0.0	5.591362798231061E-5	0.0
12	0.0	2.7956813991155304E-5	0.0	5.591362798231061E-5	0.0
13	0.0	2.7956813991155304E-5	0.0	5.591362798231061E-5	0.0
14	0.0	2.7956813991155304E-5	0.0	9.784884896904356E-5	0.0
15	0.0	2.7956813991155304E-5	0.0	1.8171929094250948E-4	0.0
16	0.0	5.591362798231061E-5	0.0	2.6558973291597535E-4	0.0
17	0.0	5.591362798231061E-5	0.0	4.612874308540625E-4	0.0
18	0.0	6.989203497788826E-5	0.0	5.591362798231061E-4	0.0
19	0.0	6.989203497788826E-5	0.0	7.268771637700379E-4	0.0
20	0.0	6.989203497788826E-5	0.0	9.784884896904356E-4	0.0
21	0.0	6.989203497788826E-5	0.0	0.0012999918505887215	0.0
22	0.0	6.989203497788826E-5	0.0	0.002124717863327803	0.0
23	0.0	9.784884896904356E-5	0.0	0.003131163167009394	0.0
24	0.0	9.784884896904356E-5	0.0	0.0047107231575096685	0.0
25	0.0	9.784884896904356E-5	0.0	0.005884909345138191	0.0
26	0.0	9.784884896904356E-5	0.0	0.0097569280829132	0.0
27	0.0	1.1182725596462121E-4	0.0	0.027285850455367574	0.0
28	0.0	1.1182725596462121E-4	0.0	0.09653487871145926	0.0
29	0.0	1.3978406995577653E-4	0.0	0.2028406639128273	0.0
30	0.0	1.3978406995577653E-4	0.0	0.34585374588458223	0.0
31	0.0	1.3978406995577653E-4	0.0	0.7523038860949937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGATC	1735	0.0	21.21902	8
TCTAGAC	2245	0.0	21.095768	3
CTAGACA	2300	0.0	20.993479	4
GGTATCA	5105	0.0	20.801176	1
TAGAACT	2515	0.0	20.155071	4
GTCTAGA	2150	0.0	20.134882	1
ACATGTA	1920	0.0	19.270834	8
ACGGAGT	1780	0.0	19.22753	26
GAATCTA	810	0.0	19.185184	13
CTAGAAC	2400	0.0	18.885416	3
GTTCTAG	2595	0.0	18.678228	1
CTCTAGT	2350	0.0	18.342554	27
AACGGAG	1865	0.0	18.152817	25
AGTGCGC	1155	0.0	17.939394	29
ACAACGG	1910	0.0	17.821991	23
AATCTAC	875	0.0	17.76	14
CTTATAC	6380	0.0	17.65909	37
GGCACTA	1025	0.0	17.146341	12
GACATGT	2170	0.0	17.135944	7
TCTAGTT	2595	0.0	17.038536	28
>>END_MODULE