FastQCFastQC Report
Fri 10 Feb 2017
ERR1631375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317975
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA7200.22643289566789843No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5960.18743611919176034No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC5020.15787404670178473No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC4600.14466546112115733No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC4340.1364887176664832No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC4230.13302932620489033No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG3860.1213931912886233No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG3630.11415991823256544No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT3380.10629766491076342No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT3190.10032235238619387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.023.8958321
GACTGCG400.001928132123.1250027
AAAGGCC502.6961492E-422.24
TATACAC1550.021.4838737
ATACACA1059.749783E-1021.14285737
TAGGAGT806.927585E-720.81254
GCCCCTA450.003819815320.55555527
GGTGTGC450.003819815320.5555558
TAGAATA450.003819815320.5555552
TAGAAGG450.003819815320.55555511
AGTCGGT2100.020.26190411
GCAGTCG2150.019.7906979
AGGACCG1052.2440872E-819.380955
TAGAGTC609.216799E-418.57
TAGGGGC701.2156807E-418.510
AAGGCCG609.216799E-418.55
CAGTCGG2350.018.10638410
TCGGTGA2500.017.7613
ATAGGAG852.7130984E-517.4117643
TGACCCG852.7130984E-517.41176413
Produced by FastQC (version 0.11.5)