##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631375.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	317975
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81041591320072	34.0	31.0	34.0	31.0	34.0
2	32.99798726314962	34.0	33.0	34.0	31.0	34.0
3	33.10153628429908	34.0	33.0	34.0	31.0	34.0
4	36.432549728752264	37.0	37.0	37.0	35.0	37.0
5	36.413359540844404	37.0	37.0	37.0	35.0	37.0
6	36.465215818853686	37.0	37.0	37.0	35.0	37.0
7	36.45146002044186	37.0	37.0	37.0	35.0	37.0
8	36.43148360720183	37.0	37.0	37.0	35.0	37.0
9	38.286824435883325	39.0	39.0	39.0	37.0	39.0
10	38.246865319600595	39.0	39.0	39.0	37.0	39.0
11	38.305530308986555	39.0	39.0	39.0	37.0	39.0
12	38.07861938831669	39.0	39.0	39.0	37.0	39.0
13	38.206915638021854	39.0	39.0	39.0	37.0	39.0
14	39.715153707052444	41.0	40.0	41.0	38.0	41.0
15	39.73256388080824	41.0	40.0	41.0	38.0	41.0
16	39.70116204104096	41.0	40.0	41.0	38.0	41.0
17	39.685981602327224	41.0	40.0	41.0	37.0	41.0
18	39.6682946772545	41.0	40.0	41.0	37.0	41.0
19	39.68304111958487	41.0	40.0	41.0	37.0	41.0
20	39.65880336504442	41.0	40.0	41.0	37.0	41.0
21	39.622583536441546	41.0	40.0	41.0	37.0	41.0
22	39.576611368818305	41.0	40.0	41.0	37.0	41.0
23	39.49360169824672	41.0	39.0	41.0	37.0	41.0
24	39.46594543596195	41.0	39.0	41.0	37.0	41.0
25	39.439694944571116	41.0	39.0	41.0	37.0	41.0
26	39.37726236339335	41.0	39.0	41.0	36.0	41.0
27	39.29662709332495	41.0	39.0	41.0	36.0	41.0
28	39.19467568205047	41.0	39.0	41.0	36.0	41.0
29	39.11629530623477	41.0	39.0	41.0	35.0	41.0
30	39.06323453101659	40.0	39.0	41.0	35.0	41.0
31	39.0237943234531	40.0	39.0	41.0	35.0	41.0
32	38.94262127525749	40.0	39.0	41.0	35.0	41.0
33	38.85573079644626	40.0	38.0	41.0	35.0	41.0
34	38.817168016353484	40.0	38.0	41.0	35.0	41.0
35	38.70349555782688	40.0	38.0	41.0	35.0	41.0
36	38.6573881594465	40.0	38.0	41.0	35.0	41.0
37	38.59080430851482	40.0	38.0	41.0	35.0	41.0
38	38.49284692192782	40.0	38.0	41.0	35.0	41.0
39	38.40525198521896	40.0	38.0	41.0	35.0	41.0
40	38.33012972717981	40.0	38.0	41.0	34.0	41.0
41	38.23390518122494	40.0	37.0	41.0	34.0	41.0
42	38.13007626385722	40.0	37.0	41.0	34.0	41.0
43	37.17056372356317	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	2.0
16	2.0
17	2.0
18	17.0
19	25.0
20	35.0
21	50.0
22	93.0
23	169.0
24	267.0
25	352.0
26	546.0
27	724.0
28	1032.0
29	1345.0
30	1870.0
31	2483.0
32	3209.0
33	4430.0
34	6826.0
35	9931.0
36	16167.0
37	30023.0
38	71855.0
39	166518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.266923500275176	16.423303718845823	12.545011400267317	27.764761380611684
2	21.53817123987735	18.37848887491155	31.73173991666011	28.35159996855099
3	21.299473229027438	19.184527085462694	27.972953848573002	31.543045836936866
4	15.94999606887334	14.987027282019028	31.173519930812173	37.88945671829546
5	17.876562622847707	33.229341929396966	31.85502004874597	17.039075399009356
6	37.45671829546348	31.358440128940956	15.067536756034281	16.117304819561287
7	32.70791728909506	27.308750687947164	18.858400817674344	21.124931205283435
8	29.941347590219358	30.024058495164713	18.415913200723327	21.6186807138926
9	27.969808947244278	13.685038131928613	17.588489661136883	40.75666325969023
10	20.120135230757136	23.46693922478182	28.89000707602799	27.522918468433055
11	39.68865476845664	19.85942291060618	18.126582278481013	22.32534004245617
12	25.225882537935373	23.024766097963674	25.016432109442565	26.732919254658384
13	33.34570327855963	16.30694236968315	22.947401525277144	27.399952826480067
14	25.317399166601145	18.68668920512619	21.0047959745263	34.99111565374636
15	29.668999135152134	24.033964934350184	19.003380768928373	27.29365516156931
16	28.938438556490294	22.75178866263071	20.944099378881987	27.365673401997014
17	27.5798411824829	23.29900149382813	21.445396650680085	27.675760673008888
18	27.547134208664204	20.62080352228949	23.59462221872789	28.23744005031842
19	27.961946693922478	22.96155358125639	21.89197263935844	27.184527085462694
20	28.71232015095526	20.46230049532196	22.494535733941348	28.330843619781433
21	30.340750058966897	21.417721518987342	21.625599496815788	26.61592892522997
22	30.146395156851952	20.990014938281313	20.765468983410646	28.098120921456086
23	27.913515213460176	21.07146788269518	21.979400896296877	29.035616007547766
24	27.541787876405376	22.14765311738344	22.236024844720497	28.074534161490682
25	27.682679455932067	21.144115103388632	22.00361663652803	29.16958880415127
26	28.399402468747542	22.21998584794402	21.9768849752339	27.403726708074533
27	28.96517021778442	20.80194983882381	21.91147102759651	28.321408915795267
28	27.535812563880807	21.39319128862332	22.675682050475665	28.395314097020208
29	26.35773252614199	21.67906281940404	23.832691249312052	28.130513405141915
30	25.762717194748014	23.374793615850304	23.84275493356396	27.019734255837726
31	28.06635741803601	22.50428492806038	21.653903608774275	27.775454045129333
32	26.400188694079723	21.714600204418584	23.245223681107007	28.639987420394686
33	26.300180831826403	21.031213145687556	23.757213617422753	28.911392405063292
34	27.58927588646906	20.836543753439734	23.43328878056451	28.140891579526695
35	27.067222265901407	20.705086877899205	24.39908797861467	27.828602877584718
36	25.231857850459942	21.662709332494693	24.857929082475035	28.24750373457033
37	26.69172104725214	19.663181067694	25.47841811463165	28.166679770422203
38	24.892522997090964	19.045522446733234	27.13892601619624	28.923028539979555
39	25.376837801713968	18.616557905495714	28.03113452315434	27.97546976963598
40	24.120449720890008	19.06313389417407	28.427392090573157	28.389024294362763
41	22.723799040805094	19.44115103388631	30.395156851953768	27.43989307335482
42	23.370705244122966	19.37007626385722	29.741646355845585	27.51757213617423
43	21.031213145687556	19.32478968472364	30.507429829389103	29.136567340199704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	9.5
4	17.0
5	17.0
6	29.0
7	41.0
8	41.5
9	42.0
10	64.0
11	86.0
12	86.0
13	149.5
14	213.0
15	340.0
16	467.0
17	446.0
18	425.0
19	425.0
20	500.5
21	576.0
22	533.5
23	491.0
24	580.5
25	670.0
26	670.0
27	823.5
28	977.0
29	1458.5
30	1940.0
31	2363.5
32	2787.0
33	2787.0
34	3461.5
35	4136.0
36	5260.5
37	6385.0
38	7856.0
39	9327.0
40	9327.0
41	10626.0
42	11925.0
43	13204.5
44	14484.0
45	16544.0
46	18604.0
47	18604.0
48	21133.0
49	23662.0
50	25032.0
51	26402.0
52	28335.0
53	30268.0
54	30268.0
55	28466.5
56	26665.0
57	26193.5
58	25722.0
59	25032.5
60	24343.0
61	24343.0
62	23305.5
63	22268.0
64	20946.0
65	19624.0
66	17473.0
67	15322.0
68	15322.0
69	13111.0
70	10900.0
71	9304.0
72	7708.0
73	5919.5
74	4131.0
75	4131.0
76	3328.0
77	2525.0
78	2176.0
79	1827.0
80	1573.5
81	1320.0
82	1320.0
83	1043.5
84	767.0
85	597.0
86	427.0
87	356.5
88	286.0
89	286.0
90	220.0
91	154.0
92	98.5
93	43.0
94	27.0
95	11.0
96	11.0
97	7.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	317975.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.97292857254493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.91915211432669	54.82634039170644
2	4.945955769732971	6.031388157417899
3	1.5353701178960373	2.8084803759268486
4	0.7845790770179116	1.9135233609010545
5	0.4885285836498731	1.4893509218265129
6	0.30596571055699173	1.1193375249263646
7	0.2429764374914121	1.037048947758271
8	0.2007103201590502	0.9790316811864312
9	0.14366864251560352	0.7883908090396546
>10	1.2179177050112555	14.971042070247654
>50	0.1443124291783111	6.138275426775443
>100	0.06931134591359742	7.3244262709465575
>500	0.0015517465503044196	0.5733640613408906
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	720	0.22643289566789843	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	596	0.18743611919176034	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	502	0.15787404670178473	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	460	0.14466546112115733	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	434	0.1364887176664832	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	423	0.13302932620489033	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	386	0.1213931912886233	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	363	0.11415991823256544	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	338	0.10629766491076342	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	319	0.10032235238619387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	6.289802657441623E-4	0.0
17	0.0	0.0	0.0	0.0015724506643604056	0.0
18	0.0	3.1449013287208113E-4	0.0	0.0015724506643604056	0.0
19	0.0	3.1449013287208113E-4	0.0	0.002515921062976649	0.0
20	0.0	3.1449013287208113E-4	0.0	0.0031449013287208113	0.0
21	0.0	3.1449013287208113E-4	0.0	0.0037738815944649735	0.0
22	0.0	3.1449013287208113E-4	0.0	0.005031842125953298	0.0
23	0.0	3.1449013287208113E-4	0.0	0.007547763188929947	0.0
24	0.0	3.1449013287208113E-4	0.0	0.016982467175092383	0.0
25	0.0	3.1449013287208113E-4	0.0	0.021070838902429435	0.0
26	0.0	3.1449013287208113E-4	0.0	0.02987656262284771	0.0
27	0.0	3.1449013287208113E-4	0.0	0.06289802657441623	0.0
28	0.0	3.1449013287208113E-4	0.0	0.22957779699661923	0.0
29	0.0	3.1449013287208113E-4	0.0	0.4626149854548314	0.0
30	0.0	3.1449013287208113E-4	0.0	0.7859108420473307	0.0
31	0.0	3.1449013287208113E-4	0.0	1.7394449249154809	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	480	0.0	23.895832	1
GACTGCG	40	0.0019281321	23.125002	7
AAAGGCC	50	2.6961492E-4	22.2	4
TATACAC	155	0.0	21.48387	37
ATACACA	105	9.749783E-10	21.142857	37
TAGGAGT	80	6.927585E-7	20.8125	4
GCCCCTA	45	0.0038198153	20.555555	27
GGTGTGC	45	0.0038198153	20.555555	8
TAGAATA	45	0.0038198153	20.555555	2
TAGAAGG	45	0.0038198153	20.555555	11
AGTCGGT	210	0.0	20.261904	11
GCAGTCG	215	0.0	19.790697	9
AGGACCG	105	2.2440872E-8	19.38095	5
TAGAGTC	60	9.216799E-4	18.5	7
TAGGGGC	70	1.2156807E-4	18.5	10
AAGGCCG	60	9.216799E-4	18.5	5
CAGTCGG	235	0.0	18.106384	10
TCGGTGA	250	0.0	17.76	13
ATAGGAG	85	2.7130984E-5	17.411764	3
TGACCCG	85	2.7130984E-5	17.411764	13
>>END_MODULE