FastQCFastQC Report
Fri 10 Feb 2017
ERR1631374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734733
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.2958897994237362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17600.2395427999014608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15260.20769449582365296No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11350.15447788516372615No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8760.11922698449640891No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8740.11895477676924814No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC8690.11827425745134627No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT8670.11800204972418553No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8130.11065244109084524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA701.920962E-723.7857132
TACAATT502.700817E-422.19999914
TATCGCC603.7239315E-521.58333233
GTACTAT705.0977887E-621.1428571
GTCATAG450.003824514820.5555551
GGTATCA9950.020.4522611
TCTATCG656.897799E-519.92307731
TACCCTC701.2184377E-418.55
TATACTG609.232493E-418.55
TCGGGTC500.007032526718.49999837
GTTAAGG500.007032526718.4999981
CGGATAG500.007032526718.4999985
GTACTAA500.007032526718.4999981
GACTATG500.007032526718.4999987
ATACACA2800.017.83928537
CTTATAC12050.017.34854737
CTATCGC752.0659875E-417.26666632
CGCCTAT752.0659875E-417.26666636
GAACTAG650.001579200617.0769231
TCGTTTA1708.54925E-1116.3235330