##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631374.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734733
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5061961283895	34.0	31.0	34.0	31.0	34.0
2	32.69681639452699	34.0	31.0	34.0	31.0	34.0
3	32.804586155787206	34.0	31.0	34.0	31.0	34.0
4	36.25916625495248	37.0	37.0	37.0	35.0	37.0
5	36.15316312184154	37.0	35.0	37.0	35.0	37.0
6	36.18323390946099	37.0	36.0	37.0	35.0	37.0
7	36.18334687566776	37.0	36.0	37.0	35.0	37.0
8	36.175597121675494	37.0	36.0	37.0	35.0	37.0
9	37.95957443043935	39.0	38.0	39.0	35.0	39.0
10	37.87610329194415	39.0	38.0	39.0	35.0	39.0
11	37.940424616833596	39.0	38.0	39.0	35.0	39.0
12	37.70937878113546	39.0	38.0	39.0	35.0	39.0
13	37.85629337460003	39.0	38.0	39.0	35.0	39.0
14	39.25620327384233	40.0	39.0	41.0	36.0	41.0
15	39.222242637801756	40.0	39.0	41.0	36.0	41.0
16	39.19544242602415	40.0	39.0	41.0	36.0	41.0
17	39.19221948653456	40.0	39.0	41.0	36.0	41.0
18	39.14613607936489	40.0	39.0	41.0	36.0	41.0
19	39.15265953754629	40.0	39.0	41.0	36.0	41.0
20	39.11179435250629	40.0	39.0	41.0	36.0	41.0
21	39.08643139752808	40.0	39.0	41.0	36.0	41.0
22	39.02339489311083	40.0	39.0	41.0	36.0	41.0
23	38.94889299922557	40.0	38.0	41.0	35.0	41.0
24	38.89567911064292	40.0	38.0	41.0	35.0	41.0
25	38.884488650979335	40.0	38.0	41.0	35.0	41.0
26	38.77515233424931	40.0	38.0	41.0	35.0	41.0
27	38.707199758279536	40.0	38.0	41.0	35.0	41.0
28	38.59906659970357	40.0	38.0	41.0	35.0	41.0
29	38.507740907241136	40.0	38.0	41.0	35.0	41.0
30	38.43873216528998	40.0	38.0	41.0	34.0	41.0
31	38.382779866972086	40.0	38.0	41.0	34.0	41.0
32	38.29578091633287	40.0	38.0	41.0	34.0	41.0
33	38.182923592652024	40.0	38.0	41.0	34.0	41.0
34	38.13000504945334	40.0	38.0	41.0	34.0	41.0
35	38.002962981110144	40.0	37.0	41.0	33.0	41.0
36	37.945034454693065	40.0	37.0	41.0	33.0	41.0
37	37.866164987825506	40.0	37.0	41.0	33.0	41.0
38	37.76983883941514	40.0	37.0	41.0	33.0	41.0
39	37.680877271063096	40.0	37.0	41.0	33.0	41.0
40	37.55038224770087	40.0	36.0	41.0	33.0	41.0
41	37.43665794240901	40.0	36.0	41.0	33.0	41.0
42	37.302239044659764	40.0	36.0	41.0	33.0	41.0
43	36.284394467105734	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	4.0
15	4.0
16	10.0
17	18.0
18	25.0
19	54.0
20	122.0
21	191.0
22	335.0
23	540.0
24	849.0
25	1251.0
26	1917.0
27	2674.0
28	3568.0
29	5065.0
30	6809.0
31	8619.0
32	11402.0
33	15350.0
34	22389.0
35	32237.0
36	51720.0
37	96594.0
38	210388.0
39	262595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99793258231222	18.094872559147337	12.485079613955001	26.42211524458545
2	20.011351062222605	19.525732476967825	33.367359299228426	27.095557161581148
3	20.61143299674848	21.47800629616473	28.432097101940435	29.47846360514636
4	15.208926235789056	15.491750064309077	32.6171548031734	36.68216889672847
5	16.64985783951449	34.62577562189258	31.68579606469289	17.03857047390004
6	35.205441976881396	33.361506833094474	15.523734472250464	15.909316717773667
7	30.7556622609846	29.09043149007871	19.973650292010838	20.180255956925848
8	28.812235192920422	31.309741089620314	19.04623856557416	20.831785151885107
9	27.826298805144184	14.012981586508296	19.029497790353776	39.13122181799375
10	19.84584876410887	24.500056483103386	29.36889999496416	26.28519475782359
11	38.19006360133545	20.680029343992988	19.740640477561236	21.389266577110323
12	24.20076408709014	24.56388919512258	25.975422364314653	25.259924353472623
13	31.101229970615172	17.71201239089574	24.14727526870305	27.039482369786032
14	24.130806701209828	19.77085553527608	23.10240590799651	32.99593185551758
15	27.4114542289512	25.659933608535347	20.6986755733035	26.229936589209956
16	26.90283409075133	24.180348507553084	22.247809748575335	26.669007653120246
17	25.735607356686035	24.823167055243196	23.274441191562104	26.166784396508664
18	25.906553809342984	22.474694889163818	24.97301740904519	26.645733892448003
19	26.40088304186691	23.83437248633177	24.123729300303648	25.64101517149767
20	26.62735987086465	22.826251168791927	23.974015050365235	26.572373909978182
21	28.9066912742452	23.23674042135034	23.221224580902177	24.635343723502277
22	27.963899811223943	23.08362357482242	23.027684886890885	25.924791727062757
23	26.30343267554336	22.94534204942476	23.87751741108675	26.873707863945135
24	26.033674817927054	23.82824781247065	23.787144445669377	26.350932923932913
25	26.126633756752454	22.755204952002973	23.98013972422635	27.13802156701822
26	26.218095553078463	24.187834220050007	24.303250296366162	25.290819930505364
27	27.337413727163472	22.769495857678912	23.47587490966106	26.417215505496554
28	25.57037726629946	23.10145318095145	25.010309867666216	26.31785968508288
29	25.457547163391325	23.277979892015193	25.111571142170014	26.152901802423468
30	24.895982622258696	23.917259739252216	25.16124905237685	26.025508586112235
31	25.95813717363995	23.60585409938032	23.69649927252485	26.739509454454886
32	25.094558159222462	22.97501269168528	24.474468956750275	27.455960192341983
33	24.86399821431731	22.730978464285663	25.221951375533695	27.18307194586333
34	26.26763735942172	22.64183043364052	25.12844802125398	25.962084185683782
35	26.23755840557046	22.346076738080363	25.63284893968285	25.783515916666328
36	24.65330943349489	23.09873110367984	25.267273962105964	26.98068550071931
37	24.91598989020501	21.89856723462809	25.95514288864118	27.23029998652572
38	24.31332198227111	21.15816221675085	27.341905154661628	27.186610646316417
39	24.278751600921694	21.147410011528	27.50033005186918	27.073508335681122
40	23.916034804479995	21.61179639406424	28.157167297508074	26.315001503947695
41	22.56887876276144	21.641739244051923	29.375977395870333	26.413404597316305
42	23.18896796523363	22.229163519264823	28.683753145700546	25.898115369801
43	21.954233714832462	20.961628237740783	29.110438758025026	27.973699289401726
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	119.0
2	86.0
3	194.5
4	303.0
5	303.0
6	407.5
7	512.0
8	494.0
9	476.0
10	722.0
11	968.0
12	968.0
13	1536.0
14	2104.0
15	3253.5
16	4403.0
17	4017.5
18	3632.0
19	3632.0
20	3932.0
21	4232.0
22	3591.0
23	2950.0
24	3449.0
25	3948.0
26	3948.0
27	4759.5
28	5571.0
29	6803.5
30	8036.0
31	9683.0
32	11330.0
33	11330.0
34	14655.5
35	17981.0
36	21736.0
37	25491.0
38	28668.5
39	31846.0
40	31846.0
41	34562.0
42	37278.0
43	39490.5
44	41703.0
45	44015.5
46	46328.0
47	46328.0
48	49124.0
49	51920.0
50	52857.5
51	53795.0
52	55660.0
53	57525.0
54	57525.0
55	56240.5
56	54956.0
57	52929.5
58	50903.0
59	48598.5
60	46294.0
61	46294.0
62	43915.0
63	41536.0
64	37925.5
65	34315.0
66	30584.0
67	26853.0
68	26853.0
69	23242.5
70	19632.0
71	17456.0
72	15280.0
73	13225.5
74	11171.0
75	11171.0
76	9694.5
77	8218.0
78	6847.5
79	5477.0
80	4159.5
81	2842.0
82	2842.0
83	2276.5
84	1711.0
85	1522.0
86	1333.0
87	1176.5
88	1020.0
89	1020.0
90	754.0
91	488.0
92	292.0
93	96.0
94	63.0
95	30.0
96	30.0
97	17.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	734733.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.375848607089424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.52951243224301	46.36808340410601
2	5.635014501458461	5.902773328542837
3	1.685855229545194	2.6489429482838713
4	0.8649940615882682	1.8121919206311405
5	0.5600700277659506	1.4667071491818897
6	0.3939577220133559	1.2380322003459208
7	0.29296057992408714	1.0740841287364375
8	0.2101950837890051	0.8807316709189925
9	0.18096434024438085	0.8530344799129359
>10	1.3116804854628565	13.694019251581613
>50	0.1830181319986445	6.644423635506444
>100	0.14448792315881973	14.382268991822226
>500	0.006248126406862897	2.1354486255850036
>1k	0.0010413544011438163	0.8992582648447162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2174	0.2958897994237362	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1760	0.2395427999014608	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1526	0.20769449582365296	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1135	0.15447788516372615	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	876	0.11922698449640891	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	874	0.11895477676924814	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	869	0.11827425745134627	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	867	0.11800204972418553	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	813	0.11065244109084524	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.3610386358037544E-4	0.0	0.0	0.0	0.0
6	1.3610386358037544E-4	0.0	0.0	0.0	0.0
7	2.722077271607509E-4	0.0	0.0	0.0	0.0
8	2.722077271607509E-4	0.0	0.0	1.3610386358037544E-4	0.0
9	2.722077271607509E-4	0.0	0.0	1.3610386358037544E-4	0.0
10	2.722077271607509E-4	0.0	0.0	1.3610386358037544E-4	0.0
11	4.083115907411264E-4	0.0	0.0	1.3610386358037544E-4	0.0
12	4.083115907411264E-4	0.0	0.0	5.444154543215018E-4	0.0
13	5.444154543215018E-4	0.0	0.0	5.444154543215018E-4	0.0
14	5.444154543215018E-4	0.0	0.0	8.166231814822528E-4	0.0
15	8.166231814822528E-4	0.0	0.0	0.0010888309086430036	0.0
16	8.166231814822528E-4	0.0	0.0	0.0019054540901252564	0.0
17	9.527270450626282E-4	0.0	0.0	0.002177661817286007	0.0
18	9.527270450626282E-4	0.0	0.0	0.002177661817286007	0.0
19	9.527270450626282E-4	0.0	0.0	0.0027220772716075093	0.0
20	9.527270450626282E-4	0.0	0.0	0.003266492725929011	0.0
21	9.527270450626282E-4	0.0	0.0	0.004083115907411263	0.0
22	9.527270450626282E-4	0.0	0.0	0.005444154543215019	0.0
23	9.527270450626282E-4	1.3610386358037544E-4	0.0	0.007621816360501026	0.0
24	0.0010888309086430036	1.3610386358037544E-4	0.0	0.013065970903716044	0.0
25	0.0010888309086430036	1.3610386358037544E-4	0.0	0.016332463629645053	0.0
26	0.0010888309086430036	1.3610386358037544E-4	0.0	0.024634799308047958	0.0
27	0.0010888309086430036	1.3610386358037544E-4	0.0	0.07050180133463449	0.0
28	0.001224934772223379	1.3610386358037544E-4	0.0	0.2683968189805004	0.0
29	0.001224934772223379	1.3610386358037544E-4	0.0	0.5418294809134747	0.0
30	0.001224934772223379	1.3610386358037544E-4	0.0	0.9167956250774091	0.0
31	0.001224934772223379	1.3610386358037544E-4	0.0	1.7877242481282316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGA	70	1.920962E-7	23.785713	2
TACAATT	50	2.700817E-4	22.199999	14
TATCGCC	60	3.7239315E-5	21.583332	33
GTACTAT	70	5.0977887E-6	21.142857	1
GTCATAG	45	0.0038245148	20.555555	1
GGTATCA	995	0.0	20.452261	1
TCTATCG	65	6.897799E-5	19.923077	31
TACCCTC	70	1.2184377E-4	18.5	5
TATACTG	60	9.232493E-4	18.5	5
TCGGGTC	50	0.0070325267	18.499998	37
GTTAAGG	50	0.0070325267	18.499998	1
CGGATAG	50	0.0070325267	18.499998	5
GTACTAA	50	0.0070325267	18.499998	1
GACTATG	50	0.0070325267	18.499998	7
ATACACA	280	0.0	17.839285	37
CTTATAC	1205	0.0	17.348547	37
CTATCGC	75	2.0659875E-4	17.266666	32
CGCCTAT	75	2.0659875E-4	17.266666	36
GAACTAG	65	0.0015792006	17.076923	1
TCGTTTA	170	8.54925E-11	16.32353	30
>>END_MODULE