FastQCFastQC Report
Fri 10 Feb 2017
ERR1631373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221344
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50670.41487083082243825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39390.32251355883354726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39040.3196478633374381No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA24240.19846988235910604No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC21850.1789012759713889No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC16750.13714399874236907No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA16420.13444205727460895No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC15770.1291200513532633No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC15750.12895629732491418No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.12740063405559776No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14850.12158736604920481No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT13820.1131540335892263No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC13700.11217150941913172No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG13100.10725888856865878No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC12450.10193688264731311No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC12270.10046309639217124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA502.7022362E-422.22
AGTCGGT11900.021.92016811
TAGCTCG603.726533E-521.58333210
GCAGTCG12300.021.3577239
CAGTCGG12350.021.27125410
GGCAGTC12600.020.996038
GGGCAGT13100.020.0534367
CGGTGAT13450.019.25650614
TCGGTGA13550.019.11439113
GTCGGTG13950.018.96415712
AACTATA609.237268E-418.52
TAGATAA3150.018.2063484
GTGATTC14300.017.98251716
GGTGATT14450.017.92387615
GGTATCA42850.017.8308051
GGTATTA1351.1514203E-917.8148161
GGTACAT1258.580173E-917.761
TGGGCAG15650.017.731636
GCTCTAT1705.456968E-1217.4117641
CCGCTTA4150.017.38554225
Produced by FastQC (version 0.11.5)