##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631373.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1221344
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83084618256609	34.0	33.0	34.0	31.0	34.0
2	32.99325906542301	34.0	33.0	34.0	31.0	34.0
3	33.08108035082663	34.0	33.0	34.0	31.0	34.0
4	36.42926399114418	37.0	37.0	37.0	35.0	37.0
5	36.42367424738649	37.0	37.0	37.0	35.0	37.0
6	36.48343709880263	37.0	37.0	37.0	35.0	37.0
7	36.470981148636255	37.0	37.0	37.0	35.0	37.0
8	36.45865865800299	37.0	37.0	37.0	35.0	37.0
9	38.312488537218016	39.0	39.0	39.0	37.0	39.0
10	38.276227663950536	39.0	39.0	39.0	37.0	39.0
11	38.32516064270181	39.0	39.0	39.0	37.0	39.0
12	38.109653791233264	39.0	39.0	39.0	37.0	39.0
13	38.23839229570047	39.0	39.0	39.0	37.0	39.0
14	39.75762848141064	41.0	40.0	41.0	38.0	41.0
15	39.74677486441166	41.0	40.0	41.0	38.0	41.0
16	39.7250537113213	41.0	40.0	41.0	38.0	41.0
17	39.71453579008043	41.0	40.0	41.0	38.0	41.0
18	39.729826322477535	41.0	40.0	41.0	38.0	41.0
19	39.74907151465926	41.0	40.0	41.0	38.0	41.0
20	39.73370238032856	41.0	40.0	41.0	38.0	41.0
21	39.70896569680614	41.0	40.0	41.0	37.0	41.0
22	39.65632696439332	41.0	40.0	41.0	37.0	41.0
23	39.59554801923127	41.0	40.0	41.0	37.0	41.0
24	39.56602234914979	41.0	40.0	41.0	37.0	41.0
25	39.54599850656326	41.0	39.0	41.0	37.0	41.0
26	39.47964046165536	41.0	39.0	41.0	37.0	41.0
27	39.40865145282574	41.0	39.0	41.0	36.0	41.0
28	39.30242175832525	41.0	39.0	41.0	36.0	41.0
29	39.209530648203945	41.0	39.0	41.0	35.0	41.0
30	39.13306242958577	41.0	39.0	41.0	35.0	41.0
31	39.05449406555401	40.0	39.0	41.0	35.0	41.0
32	38.970716685880475	40.0	39.0	41.0	35.0	41.0
33	38.8601958170671	40.0	38.0	41.0	35.0	41.0
34	38.7947498820971	40.0	38.0	41.0	35.0	41.0
35	38.683660786805355	40.0	38.0	41.0	35.0	41.0
36	38.61177686221081	40.0	38.0	41.0	35.0	41.0
37	38.52628825294102	40.0	38.0	41.0	35.0	41.0
38	38.42021330599733	40.0	38.0	41.0	35.0	41.0
39	38.30799512668012	40.0	38.0	41.0	35.0	41.0
40	38.184242113606	40.0	37.0	41.0	34.0	41.0
41	38.06181141431079	40.0	37.0	41.0	34.0	41.0
42	37.91451384704064	40.0	37.0	41.0	34.0	41.0
43	36.97574393455079	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	7.0
13	2.0
14	8.0
15	4.0
16	3.0
17	9.0
18	27.0
19	35.0
20	94.0
21	159.0
22	296.0
23	513.0
24	844.0
25	1295.0
26	1930.0
27	2980.0
28	4228.0
29	5970.0
30	7728.0
31	9923.0
32	12545.0
33	16724.0
34	25126.0
35	36648.0
36	59379.0
37	121934.0
38	263139.0
39	649793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.64058610841827	16.096857232687924	12.160783530274845	25.101773128618966
2	21.181501689941573	20.31417847879058	31.85654492100506	26.647774910262793
3	19.726956533130714	21.964573453506954	29.171879503235782	29.13659051012655
4	13.545487593994812	16.308509314329132	33.80251591689156	36.3434871747845
5	15.637363429140358	34.60409188566039	32.194615112531764	17.56392957266749
6	33.31428328136872	34.1530314145728	17.39640920166636	15.136276102392118
7	30.88949550658946	30.022827311551865	19.723517698535385	19.36415948332329
8	29.743135431131606	30.703307176356535	19.25075981869154	20.302797573820317
9	28.5415902481201	14.256180077029896	18.303197133649487	38.89903254120051
10	19.258046872953074	23.728040584798386	28.92657596876883	28.08733657347971
11	36.54719718605078	22.176798674247387	19.57532030287945	21.700683836822385
12	24.890857940105327	25.348550449341055	25.68383682238583	24.07675478816779
13	30.89571815966673	17.876372258757563	25.108978305866327	26.118931275709382
14	24.440288731102786	19.65375848245867	23.445974270967067	32.459978515471484
15	27.008033772630807	25.31596339769958	21.347875782744254	26.328127046925353
16	26.827494956375926	24.25311787669977	23.15498336259072	25.764403804333586
17	24.935972174915502	25.571010296853302	23.303508266303353	26.189509261927846
18	25.67212840935887	22.262032646003092	25.813939397909184	26.25189954672885
19	26.14889826289727	24.565969947860715	24.655461524353502	24.629670264888517
20	26.902494301359813	22.46811709068043	24.877921371865746	25.751467236094005
21	28.968169491969505	22.802420939555113	23.93690884795766	24.292500720517722
22	28.36686469987162	22.23263879791443	23.331264574108523	26.06923192810543
23	25.422894778211546	22.767541331516757	24.505544711399903	27.3040191788718
24	24.870306809547515	24.319110750124455	24.86138221500249	25.94920022532554
25	25.094404197343255	23.149006340555978	24.746345010087246	27.01024445201352
26	26.318793067309453	24.06815770167946	24.398858961930465	25.21419026908062
27	26.77452052820499	22.87922157885084	23.684891398328396	26.66136649461577
28	24.99852621374486	23.542916655749732	25.510175675321612	25.9483814551838
29	24.792523646081694	23.507791416668848	26.30118950926193	25.398495427987527
30	24.31313372808971	24.09476773128619	26.254192103125735	25.337906437498365
31	25.313097702203475	23.65017554431839	24.597656352346267	26.439070401131872
32	24.291845704404327	22.99818888044646	25.506818717740455	27.20314669740876
33	24.12375219430398	22.443553986428068	26.58595776456101	26.846736054706945
34	25.774965939162104	22.395983441192655	25.808863023030366	26.020187596614875
35	25.47734299263762	22.376906096889986	26.206294049833627	25.93945686063877
36	23.299496292608797	23.117811198155476	26.25181766971468	27.33087483952105
37	24.17738163858831	22.017302250635364	27.22025899337124	26.585057117405086
38	23.04829761312128	20.841875835145547	28.516699635811044	27.593126915922134
39	23.810326984043808	20.78112309062803	28.874092802682945	26.534457122645215
40	23.30514580658684	21.475685801870725	28.608238137658187	26.610930253884245
41	21.363023030366545	21.89497799145859	30.41010558859748	26.33189338957738
42	22.2562193779967	22.373876647365528	29.671083658658006	25.698820315979777
43	20.77989493541541	21.5709906463699	29.72667815128252	27.922436266932166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	56.5
2	95.0
3	453.0
4	811.0
5	811.0
6	1159.0
7	1507.0
8	1447.0
9	1387.0
10	1929.5
11	2472.0
12	2472.0
13	3845.5
14	5219.0
15	7828.5
16	10438.0
17	9583.5
18	8729.0
19	8729.0
20	9118.5
21	9508.0
22	7386.5
23	5265.0
24	5495.0
25	5725.0
26	5725.0
27	6575.5
28	7426.0
29	8954.5
30	10483.0
31	12925.5
32	15368.0
33	15368.0
34	20237.5
35	25107.0
36	31298.5
37	37490.0
38	44720.5
39	51951.0
40	51951.0
41	56090.0
42	60229.0
43	66435.0
44	72641.0
45	78708.0
46	84775.0
47	84775.0
48	93227.0
49	101679.0
50	101777.0
51	101875.0
52	101054.5
53	100234.0
54	100234.0
55	94013.0
56	87792.0
57	82959.0
58	78126.0
59	77538.0
60	76950.0
61	76950.0
62	71826.0
63	66702.0
64	60545.5
65	54389.0
66	49543.0
67	44697.0
68	44697.0
69	38065.5
70	31434.0
71	29124.0
72	26814.0
73	20967.0
74	15120.0
75	15120.0
76	10729.0
77	6338.0
78	5168.5
79	3999.0
80	3678.0
81	3357.0
82	3357.0
83	2678.0
84	1999.0
85	1775.0
86	1551.0
87	1282.5
88	1014.0
89	1014.0
90	769.0
91	524.0
92	297.5
93	71.0
94	48.5
95	26.0
96	26.0
97	15.5
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1221344.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.65447495975214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.98408151548335	41.04851127920909
2	5.964706143263894	5.565604668063702
3	1.8110730907152774	2.534839924831705
4	0.9158098941919643	1.70906519105889
5	0.5639388457462347	1.3155135378849614
6	0.40311336382954277	1.1284225403236112
7	0.2894026794527192	0.9451351043268444
8	0.21578018161864898	0.8053688864110422
9	0.17270490181999745	0.7251710865648772
>10	1.2974325771793531	12.253082241149716
>50	0.1819854623761826	5.97514425365178
>100	0.17755619837678463	16.72354154176297
>500	0.017826218429554414	5.610745091740494
>1k	0.0044124303043451525	3.2426183769357615
>5k	1.764972121738061E-4	0.4172362760845503
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5067	0.41487083082243825	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3939	0.32251355883354726	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3904	0.3196478633374381	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2424	0.19846988235910604	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2185	0.1789012759713889	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1675	0.13714399874236907	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1642	0.13444205727460895	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1577	0.1291200513532633	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1575	0.12895629732491418	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1556	0.12740063405559776	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1485	0.12158736604920481	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1382	0.1131540335892263	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1370	0.11217150941913172	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1310	0.10725888856865878	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1245	0.10193688264731311	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	1227	0.10046309639217124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.187701417454869E-5	0.0
3	0.0	0.0	0.0	8.187701417454869E-5	0.0
4	0.0	0.0	0.0	8.187701417454869E-5	0.0
5	0.0	8.187701417454869E-5	0.0	8.187701417454869E-5	0.0
6	0.0	8.187701417454869E-5	0.0	8.187701417454869E-5	0.0
7	0.0	8.187701417454869E-5	0.0	1.6375402834909738E-4	0.0
8	0.0	8.187701417454869E-5	0.0	1.6375402834909738E-4	0.0
9	0.0	8.187701417454869E-5	0.0	4.912620850472921E-4	0.0
10	0.0	8.187701417454869E-5	0.0	9.006471559200356E-4	0.0
11	8.187701417454869E-5	8.187701417454869E-5	0.0	0.0017194172976655226	0.0
12	8.187701417454869E-5	8.187701417454869E-5	0.0	0.0027838184819346558	0.0
13	8.187701417454869E-5	8.187701417454869E-5	0.0	0.003193203552807399	0.0
14	8.187701417454869E-5	8.187701417454869E-5	0.0	0.0043394817512510805	0.0
15	8.187701417454869E-5	8.187701417454869E-5	0.0	0.0055676369638693116	8.187701417454869E-5
16	8.187701417454869E-5	8.187701417454869E-5	0.0	0.008269578431629418	8.187701417454869E-5
17	8.187701417454869E-5	8.187701417454869E-5	0.0	0.010807765871040428	8.187701417454869E-5
18	8.187701417454869E-5	8.187701417454869E-5	0.0	0.011790290041135012	8.187701417454869E-5
19	8.187701417454869E-5	8.187701417454869E-5	0.0	0.014246600466371473	8.187701417454869E-5
20	8.187701417454869E-5	8.187701417454869E-5	0.0	0.016129771792386092	1.6375402834909738E-4
21	8.187701417454869E-5	8.187701417454869E-5	0.0	0.019814237430240782	2.4563104252364607E-4
22	8.187701417454869E-5	8.187701417454869E-5	0.0	0.026773783635077424	2.4563104252364607E-4
23	8.187701417454869E-5	8.187701417454869E-5	0.0	0.038154688605339694	2.4563104252364607E-4
24	8.187701417454869E-5	8.187701417454869E-5	0.0	0.0595245893048969	2.4563104252364607E-4
25	8.187701417454869E-5	8.187701417454869E-5	0.0	0.0697592160767155	2.4563104252364607E-4
26	8.187701417454869E-5	8.187701417454869E-5	0.0	0.0850702177273561	2.4563104252364607E-4
27	8.187701417454869E-5	8.187701417454869E-5	0.0	0.14598671627322032	3.2750805669819476E-4
28	8.187701417454869E-5	8.187701417454869E-5	0.0	0.42682487489192233	3.2750805669819476E-4
29	8.187701417454869E-5	8.187701417454869E-5	0.0	0.8616736971729505	3.2750805669819476E-4
30	8.187701417454869E-5	8.187701417454869E-5	0.0	1.5113678046479944	3.2750805669819476E-4
31	8.187701417454869E-5	8.187701417454869E-5	0.0	2.767770587156444	3.2750805669819476E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGATA	50	2.7022362E-4	22.2	2
AGTCGGT	1190	0.0	21.920168	11
TAGCTCG	60	3.726533E-5	21.583332	10
GCAGTCG	1230	0.0	21.357723	9
CAGTCGG	1235	0.0	21.271254	10
GGCAGTC	1260	0.0	20.99603	8
GGGCAGT	1310	0.0	20.053436	7
CGGTGAT	1345	0.0	19.256506	14
TCGGTGA	1355	0.0	19.114391	13
GTCGGTG	1395	0.0	18.964157	12
AACTATA	60	9.237268E-4	18.5	2
TAGATAA	315	0.0	18.206348	4
GTGATTC	1430	0.0	17.982517	16
GGTGATT	1445	0.0	17.923876	15
GGTATCA	4285	0.0	17.830805	1
GGTATTA	135	1.1514203E-9	17.814816	1
GGTACAT	125	8.580173E-9	17.76	1
TGGGCAG	1565	0.0	17.73163	6
GCTCTAT	170	5.456968E-12	17.411764	1
CCGCTTA	415	0.0	17.385542	25
>>END_MODULE